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Gene-specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms

Hunt, L; Otterhag, L; Lee, J C; Lasheen, T; Hunt, J; Seki, M; Shinozaki, K; Sommarin, Marianne LU ; Gilmour, D J and Pical, Christophe LU , et al. (2004) In New Phytologist 162(3). p.643-654
Abstract
PI-PLCs synthesise the calcium releasing second messenger IP3. We investigated the expression patterns of the Arabidopsis PI-PLC gene family and measured in vitro activity of encoded enzymes. Gene specific RT-PCR and promoter-GUS fusions were used to analyse AtPLC gene expression patterns. The five available AtPLC cDNAs were expressed as fusion proteins in Escherichia coli. All members of the AtPLC gene family were expressed in multiple organs of the plant. AtPLC1, and AtPLC5 expression was localized to the vascular cells of roots and leaves with AtPLC5::GUS also detected in the guard cells. AtPLC4::GUS was detected in pollen and cells of the stigma surface. In seedlings, AtPLC2 and AtPLC3 were constitutively expressed, while AtPLCs 1, 4... (More)
PI-PLCs synthesise the calcium releasing second messenger IP3. We investigated the expression patterns of the Arabidopsis PI-PLC gene family and measured in vitro activity of encoded enzymes. Gene specific RT-PCR and promoter-GUS fusions were used to analyse AtPLC gene expression patterns. The five available AtPLC cDNAs were expressed as fusion proteins in Escherichia coli. All members of the AtPLC gene family were expressed in multiple organs of the plant. AtPLC1, and AtPLC5 expression was localized to the vascular cells of roots and leaves with AtPLC5::GUS also detected in the guard cells. AtPLC4::GUS was detected in pollen and cells of the stigma surface. In seedlings, AtPLC2 and AtPLC3 were constitutively expressed, while AtPLCs 1, 4 and 5 were induced by abiotic stresses. AtPLC1-5 were all shown to have phospholipase C activity in the presence of calcium ions. AtPLCs showed limited tissue specific expression and expression of at least three genes was increased by abiotic stress. The differing calcium sensitivities of recombinant AtPLC protein activities may provide a mechanism for generating calcium signatures. (Less)
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Contribution to journal
publication status
published
subject
in
New Phytologist
volume
162
issue
3
pages
643 - 654
publisher
Wiley-Blackwell
external identifiers
  • wos:000221913500007
  • scopus:2542616041
ISSN
1469-8137
DOI
10.1111/j.1469-8137.2004.01069.x
language
English
LU publication?
yes
id
0b736f23-b6ef-4b3a-b66a-2de7d7004fc5 (old id 138574)
date added to LUP
2007-07-05 14:40:23
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2017-12-10 03:42:26
@article{0b736f23-b6ef-4b3a-b66a-2de7d7004fc5,
  abstract     = {PI-PLCs synthesise the calcium releasing second messenger IP3. We investigated the expression patterns of the Arabidopsis PI-PLC gene family and measured in vitro activity of encoded enzymes. Gene specific RT-PCR and promoter-GUS fusions were used to analyse AtPLC gene expression patterns. The five available AtPLC cDNAs were expressed as fusion proteins in Escherichia coli. All members of the AtPLC gene family were expressed in multiple organs of the plant. AtPLC1, and AtPLC5 expression was localized to the vascular cells of roots and leaves with AtPLC5::GUS also detected in the guard cells. AtPLC4::GUS was detected in pollen and cells of the stigma surface. In seedlings, AtPLC2 and AtPLC3 were constitutively expressed, while AtPLCs 1, 4 and 5 were induced by abiotic stresses. AtPLC1-5 were all shown to have phospholipase C activity in the presence of calcium ions. AtPLCs showed limited tissue specific expression and expression of at least three genes was increased by abiotic stress. The differing calcium sensitivities of recombinant AtPLC protein activities may provide a mechanism for generating calcium signatures.},
  author       = {Hunt, L and Otterhag, L and Lee, J C and Lasheen, T and Hunt, J and Seki, M and Shinozaki, K and Sommarin, Marianne and Gilmour, D J and Pical, Christophe and Gray, J E},
  issn         = {1469-8137},
  language     = {eng},
  number       = {3},
  pages        = {643--654},
  publisher    = {Wiley-Blackwell},
  series       = {New Phytologist},
  title        = {Gene-specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms},
  url          = {http://dx.doi.org/10.1111/j.1469-8137.2004.01069.x},
  volume       = {162},
  year         = {2004},
}