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Expression of bovine calmodulin in tobacco plants confers faster germination on saline media

Olsson, Peter LU ; Lindberg, Jenny LU ; Sommarin, Marianne LU ; Persson, Staffan LU and Bülow, Leif LU (2004) In Plant Science 166(6). p.1595-1604
Abstract
Calmodulin is a Ca2+-dependent regulatory protein in eukaryotic cells involved in a variety of intracellular activities. Many responses to abiotic stresses involve signaling by Ca2+ and Ca2+/calmodulin regulation, including water and salinity stress. To investigate how calmodulin affects germination on media with high concentrations of NaCl, transgenic tobacco plants expressing heterologous calmodulin were generated and characterized. Transgenic tobacco seeds showed significantly shortened germination times on media containing varying salt (120-160 mM) and calcium (3 and 13 mM) concentrations, compared to control seeds. Using media containing 140 mM NaCl and 13 mM CaCl2, the average germination time was shortened by 2-3 days compared to... (More)
Calmodulin is a Ca2+-dependent regulatory protein in eukaryotic cells involved in a variety of intracellular activities. Many responses to abiotic stresses involve signaling by Ca2+ and Ca2+/calmodulin regulation, including water and salinity stress. To investigate how calmodulin affects germination on media with high concentrations of NaCl, transgenic tobacco plants expressing heterologous calmodulin were generated and characterized. Transgenic tobacco seeds showed significantly shortened germination times on media containing varying salt (120-160 mM) and calcium (3 and 13 mM) concentrations, compared to control seeds. Using media containing 140 mM NaCl and 13 mM CaCl2, the average germination time was shortened by 2-3 days compared to control (P < 0.05). In addition, the germinating transgenic seeds contained higher transient levels of gamma-aminobutyric acid compared to control seeds (P < 0.05). The tobacco Ca2+/calmodulin-regulated glutamate decarboxylase, synthesizing gamma-aminobutyric acid may therefore be stimulated by the heterologous calmodulin. (C) 2004 Elsevier Ireland Ltd. All rights reserved. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
NaCl, Calcium, γ-Amino butyric acid, Glutamate decarboxylase, Nicotiana tabacum
in
Plant Science
volume
166
issue
6
pages
1595 - 1604
publisher
Elsevier
external identifiers
  • wos:000221136600023
  • scopus:1942477681
ISSN
0168-9452
DOI
10.1016/j.plantsci.2004.02.013
language
English
LU publication?
yes
id
019e10f6-abc7-458a-bec3-44d261c3fa9c (old id 138593)
date added to LUP
2007-07-09 11:13:56
date last changed
2017-07-06 14:30:20
@article{019e10f6-abc7-458a-bec3-44d261c3fa9c,
  abstract     = {Calmodulin is a Ca2+-dependent regulatory protein in eukaryotic cells involved in a variety of intracellular activities. Many responses to abiotic stresses involve signaling by Ca2+ and Ca2+/calmodulin regulation, including water and salinity stress. To investigate how calmodulin affects germination on media with high concentrations of NaCl, transgenic tobacco plants expressing heterologous calmodulin were generated and characterized. Transgenic tobacco seeds showed significantly shortened germination times on media containing varying salt (120-160 mM) and calcium (3 and 13 mM) concentrations, compared to control seeds. Using media containing 140 mM NaCl and 13 mM CaCl2, the average germination time was shortened by 2-3 days compared to control (P &lt; 0.05). In addition, the germinating transgenic seeds contained higher transient levels of gamma-aminobutyric acid compared to control seeds (P &lt; 0.05). The tobacco Ca2+/calmodulin-regulated glutamate decarboxylase, synthesizing gamma-aminobutyric acid may therefore be stimulated by the heterologous calmodulin. (C) 2004 Elsevier Ireland Ltd. All rights reserved.},
  author       = {Olsson, Peter and Lindberg, Jenny and Sommarin, Marianne and Persson, Staffan and Bülow, Leif},
  issn         = {0168-9452},
  keyword      = {NaCl,Calcium,γ-Amino butyric acid,Glutamate decarboxylase,Nicotiana tabacum},
  language     = {eng},
  number       = {6},
  pages        = {1595--1604},
  publisher    = {Elsevier},
  series       = {Plant Science},
  title        = {Expression of bovine calmodulin in tobacco plants confers faster germination on saline media},
  url          = {http://dx.doi.org/10.1016/j.plantsci.2004.02.013},
  volume       = {166},
  year         = {2004},
}