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Effect of hindered diffusion on the adsorption of proteins in agarose gel using a pore model

Gutenwik, Jan LU ; Nilsson, Bernt LU and Axelsson, Anders LU (2004) In Journal of Chromatography A 1048(2). p.161-172
Abstract
The hindered diffusion and binding of proteins of different sizes (lysozyme, BSA and IgG) in an agarose gel is described using adsorption kinetic and diffusional data together with an experimentally determined pore size distribution in the gel. The validity of the pore model, including variable diffusion coefficients and porosities is tested against experimental confocal microscopy data. No fitting parameters were used in the present model. The importance of knowing the gel structure is demonstrated especially for large proteins such as IgG. Experimental confocal microscopy data can be explained by the present model. (C) 2004 Elsevier B.V. All rights reserved.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Chromatography A
volume
1048
issue
2
pages
161 - 172
publisher
Elsevier
external identifiers
  • pmid:15481253
  • wos:000223813800003
  • scopus:4444313472
ISSN
0021-9673
DOI
10.1016/j.chroma.2004.07.052
language
English
LU publication?
yes
id
729cf787-0c01-4d48-bdc1-0e71ce353a07 (old id 138799)
date added to LUP
2007-06-25 10:57:43
date last changed
2017-12-10 04:28:32
@article{729cf787-0c01-4d48-bdc1-0e71ce353a07,
  abstract     = {The hindered diffusion and binding of proteins of different sizes (lysozyme, BSA and IgG) in an agarose gel is described using adsorption kinetic and diffusional data together with an experimentally determined pore size distribution in the gel. The validity of the pore model, including variable diffusion coefficients and porosities is tested against experimental confocal microscopy data. No fitting parameters were used in the present model. The importance of knowing the gel structure is demonstrated especially for large proteins such as IgG. Experimental confocal microscopy data can be explained by the present model. (C) 2004 Elsevier B.V. All rights reserved.},
  author       = {Gutenwik, Jan and Nilsson, Bernt and Axelsson, Anders},
  issn         = {0021-9673},
  language     = {eng},
  number       = {2},
  pages        = {161--172},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {Effect of hindered diffusion on the adsorption of proteins in agarose gel using a pore model},
  url          = {http://dx.doi.org/10.1016/j.chroma.2004.07.052},
  volume       = {1048},
  year         = {2004},
}