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Determination of protein diffusion coefficients in agarose gel with a diffusion cell

Gutenwik, Jan LU ; Nilsson, Bernt LU and Axelsson, Anders LU (2004) In Biochemical Engineering Journal 19(1). p.1-7
Abstract
A diffusion cell has been used to measure the effective diffusion coefficients of proteins. The method is applied to lysozyme and BSA at different pH and ionic strength. A parameter optimization technique is used to estimate the diffusion coefficients directly from experimental data. The importance and influence of pH and ionic strength on the diffusive properties in an agarose gel have been demonstrated. A comparison with other methods showed that there is good agreement. The diffusion cell is an accurate and easy-to-use method for the measurement of protein diffusion coefficients, in spite of the long time required for a protein diffusion experiment. For smaller proteins this time can be shortened considerably by the present parameter... (More)
A diffusion cell has been used to measure the effective diffusion coefficients of proteins. The method is applied to lysozyme and BSA at different pH and ionic strength. A parameter optimization technique is used to estimate the diffusion coefficients directly from experimental data. The importance and influence of pH and ionic strength on the diffusive properties in an agarose gel have been demonstrated. A comparison with other methods showed that there is good agreement. The diffusion cell is an accurate and easy-to-use method for the measurement of protein diffusion coefficients, in spite of the long time required for a protein diffusion experiment. For smaller proteins this time can be shortened considerably by the present parameter optimization technique. (C) 2003 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biochemical Engineering Journal
volume
19
issue
1
pages
1 - 7
publisher
The Association for the Study of Animal Behaviour / Elsevier B.V.
external identifiers
  • wos:000221565300001
  • scopus:2342609770
ISSN
1369-703X
DOI
10.1016/j.bej.2003.09.004
language
English
LU publication?
yes
id
71351cac-5a3b-466c-9b6e-2d3cba95e245 (old id 138839)
date added to LUP
2007-06-25 10:56:43
date last changed
2017-01-01 06:53:22
@article{71351cac-5a3b-466c-9b6e-2d3cba95e245,
  abstract     = {A diffusion cell has been used to measure the effective diffusion coefficients of proteins. The method is applied to lysozyme and BSA at different pH and ionic strength. A parameter optimization technique is used to estimate the diffusion coefficients directly from experimental data. The importance and influence of pH and ionic strength on the diffusive properties in an agarose gel have been demonstrated. A comparison with other methods showed that there is good agreement. The diffusion cell is an accurate and easy-to-use method for the measurement of protein diffusion coefficients, in spite of the long time required for a protein diffusion experiment. For smaller proteins this time can be shortened considerably by the present parameter optimization technique. (C) 2003 Elsevier B.V. All rights reserved.},
  author       = {Gutenwik, Jan and Nilsson, Bernt and Axelsson, Anders},
  issn         = {1369-703X},
  language     = {eng},
  number       = {1},
  pages        = {1--7},
  publisher    = {The Association for the Study of Animal Behaviour / Elsevier B.V.},
  series       = {Biochemical Engineering Journal},
  title        = {Determination of protein diffusion coefficients in agarose gel with a diffusion cell},
  url          = {http://dx.doi.org/10.1016/j.bej.2003.09.004},
  volume       = {19},
  year         = {2004},
}