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A methodology for estimation of mass transfer parameters in a detailed chromatography model based on frontal experiments

Persson, Patrik LU ; Kempe, Henrik LU ; Zacchi, Guido LU and Nilsson, Bernt LU (2004) In Chemical Engineering Research & Design 82(A4). p.517-526
Abstract
A methodology for estimation of the unknown physical parameters in a detailed model for chromatographic processes is proposed. The model describes the concentration of the solute in the mobile phase and considers external/internal mass transfer resistance. The external volume and its mixing effect are also considered. The unknown model parameters estimated are the bed void, the axial dispersion coefficient, the liquid film mass transfer coefficient, the effective diffusion coefficient and the apparent bead porosity. All the parameters in the model were subsequently determined in three classes of experiments, the external mixing behaviour, the mobile phase behaviour and the stationary phase behaviour. The estimates are based on the sum of... (More)
A methodology for estimation of the unknown physical parameters in a detailed model for chromatographic processes is proposed. The model describes the concentration of the solute in the mobile phase and considers external/internal mass transfer resistance. The external volume and its mixing effect are also considered. The unknown model parameters estimated are the bed void, the axial dispersion coefficient, the liquid film mass transfer coefficient, the effective diffusion coefficient and the apparent bead porosity. All the parameters in the model were subsequently determined in three classes of experiments, the external mixing behaviour, the mobile phase behaviour and the stationary phase behaviour. The estimates are based on the sum of the least squares of the residuals between the experimental breakthrough curves and the model response. The methodology is exemplified by frontal experiments with lysozyme, bovine serum albumin and immuno globulin gamma on a well-defined column set-up. When the mixing properties in the external volume had to be accounted for, to avoid adding broadening behaviour to the dispersion coefficient, the film mass transfer coefficient and the diffusion coefficient were also investigated. The results show that it is possible to estimate the unknown parameters using the proposed methodology. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Chemical Engineering Research & Design
volume
82
issue
A4
pages
517 - 526
publisher
IChemE
external identifiers
  • wos:000221425400013
  • scopus:2442681387
ISSN
0263-8762
DOI
10.1205/026387604323050236
language
English
LU publication?
yes
id
0abbfc43-bfa8-4e35-8dfd-41bafa009256 (old id 138844)
date added to LUP
2007-06-25 15:30:17
date last changed
2017-08-27 05:06:57
@article{0abbfc43-bfa8-4e35-8dfd-41bafa009256,
  abstract     = {A methodology for estimation of the unknown physical parameters in a detailed model for chromatographic processes is proposed. The model describes the concentration of the solute in the mobile phase and considers external/internal mass transfer resistance. The external volume and its mixing effect are also considered. The unknown model parameters estimated are the bed void, the axial dispersion coefficient, the liquid film mass transfer coefficient, the effective diffusion coefficient and the apparent bead porosity. All the parameters in the model were subsequently determined in three classes of experiments, the external mixing behaviour, the mobile phase behaviour and the stationary phase behaviour. The estimates are based on the sum of the least squares of the residuals between the experimental breakthrough curves and the model response. The methodology is exemplified by frontal experiments with lysozyme, bovine serum albumin and immuno globulin gamma on a well-defined column set-up. When the mixing properties in the external volume had to be accounted for, to avoid adding broadening behaviour to the dispersion coefficient, the film mass transfer coefficient and the diffusion coefficient were also investigated. The results show that it is possible to estimate the unknown parameters using the proposed methodology.},
  author       = {Persson, Patrik and Kempe, Henrik and Zacchi, Guido and Nilsson, Bernt},
  issn         = {0263-8762},
  language     = {eng},
  number       = {A4},
  pages        = {517--526},
  publisher    = {IChemE},
  series       = {Chemical Engineering Research & Design},
  title        = {A methodology for estimation of mass transfer parameters in a detailed chromatography model based on frontal experiments},
  url          = {http://dx.doi.org/10.1205/026387604323050236},
  volume       = {82},
  year         = {2004},
}