Advanced

Dynamics of cellulase production by glucose grown cultures of Trichoderma reesei rut-C30 as a response to addition of cellulose

Szijarto, N; Szengyel, Z; Lidén, Gunnar LU and Reczey, K (2004) In Applied Biochemistry and Biotechnology 113(1-3). p.115-124
Abstract
An economic process for the enzymatic hydrolysis of cellulose would allow utilization of cellulosic biomass for the production of easily fermentable low-cost sugars. New and more efficient fermentation processes are emerging to convert this biologic currency to a variety of commodity products with a special emphasis on fuel ethanol production. Since the cost of cellulase production currently accounts for a large fraction of the estimated total production costs of bioethanol, a significantly less expensive process for cellulase enzyme production is needed. It will most likely be desirable to obtain cellulase production on different carbon sources-including both polymeric carbohydrates and monosaccharides. The relation between enzyme... (More)
An economic process for the enzymatic hydrolysis of cellulose would allow utilization of cellulosic biomass for the production of easily fermentable low-cost sugars. New and more efficient fermentation processes are emerging to convert this biologic currency to a variety of commodity products with a special emphasis on fuel ethanol production. Since the cost of cellulase production currently accounts for a large fraction of the estimated total production costs of bioethanol, a significantly less expensive process for cellulase enzyme production is needed. It will most likely be desirable to obtain cellulase production on different carbon sources-including both polymeric carbohydrates and monosaccharides. The relation between enzyme production and growth profile of the microorganism is key for designing such processes. We conducted a careful characterization of growth and cellulase production by the soft-rot fungus Trichoderma reesei. Glucose-grown cultures of T. reesei Rut-C30 were subjected to pulse additions of Solka-floc (delignified pine pulp), and the response was monitored in terms of CO2 evolution and increased enzyme activity. There was an immediate and unexpectedly strong CO2 evolution at the point of Solka-floc addition. The time profiles of induction of cellulase activity, cellulose degradation, and CO2 evolution are analyzed and discussed herein. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied Biochemistry and Biotechnology
volume
113
issue
1-3
pages
115 - 124
publisher
Humana Press
external identifiers
  • wos:000221186200011
  • scopus:11244305401
ISSN
1559-0291
language
English
LU publication?
yes
id
a7da9638-4d34-4edd-b0aa-e271b9101870 (old id 138850)
alternative location
http://www.ingentaconnect.com/content/hum/abab/2004/00000113/F0030001/art00008
date added to LUP
2007-06-25 16:14:55
date last changed
2017-07-23 03:36:18
@article{a7da9638-4d34-4edd-b0aa-e271b9101870,
  abstract     = {An economic process for the enzymatic hydrolysis of cellulose would allow utilization of cellulosic biomass for the production of easily fermentable low-cost sugars. New and more efficient fermentation processes are emerging to convert this biologic currency to a variety of commodity products with a special emphasis on fuel ethanol production. Since the cost of cellulase production currently accounts for a large fraction of the estimated total production costs of bioethanol, a significantly less expensive process for cellulase enzyme production is needed. It will most likely be desirable to obtain cellulase production on different carbon sources-including both polymeric carbohydrates and monosaccharides. The relation between enzyme production and growth profile of the microorganism is key for designing such processes. We conducted a careful characterization of growth and cellulase production by the soft-rot fungus Trichoderma reesei. Glucose-grown cultures of T. reesei Rut-C30 were subjected to pulse additions of Solka-floc (delignified pine pulp), and the response was monitored in terms of CO2 evolution and increased enzyme activity. There was an immediate and unexpectedly strong CO2 evolution at the point of Solka-floc addition. The time profiles of induction of cellulase activity, cellulose degradation, and CO2 evolution are analyzed and discussed herein.},
  author       = {Szijarto, N and Szengyel, Z and Lidén, Gunnar and Reczey, K},
  issn         = {1559-0291},
  language     = {eng},
  number       = {1-3},
  pages        = {115--124},
  publisher    = {Humana Press},
  series       = {Applied Biochemistry and Biotechnology},
  title        = {Dynamics of cellulase production by glucose grown cultures of Trichoderma reesei rut-C30 as a response to addition of cellulose},
  volume       = {113},
  year         = {2004},
}