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Combined steam pretreatment and enzymatic hydrolysis of starch-free wheat fibers

Palmarola Adrados, Beatriz LU ; Galbe, Mats LU and Zacchi, Guido LU (2004) In Applied Biochemistry and Biotechnology 115(1-3). p.989-1002
Abstract
Steam treatment of an industrial process stream, denoted starch-free wheat fiber, was investigated to improve the formation of monomeric sugars in subsequent enzymatic hydrolysis for further bioconversion into ethanol. The solid fraction in the process stream, derived from a combined starch and ethanol factory, was rich in arabinose (21.1%), xylose (30.1%), and glucose (18.6%), in the form of polysaccharides. Various conditions of steam pretreatment (170-220degreesC for 5-30 min) were evaluated, and their effect was assessed by enzymatic hydrolysis with 2 g of Celluclast + Ultraflo mixture/ 100 g of starch-free fiber (SFF) slurry at 5% dry matter (DM). The highest overall sugar yield for the combined steam pretreatment and enzymatic... (More)
Steam treatment of an industrial process stream, denoted starch-free wheat fiber, was investigated to improve the formation of monomeric sugars in subsequent enzymatic hydrolysis for further bioconversion into ethanol. The solid fraction in the process stream, derived from a combined starch and ethanol factory, was rich in arabinose (21.1%), xylose (30.1%), and glucose (18.6%), in the form of polysaccharides. Various conditions of steam pretreatment (170-220degreesC for 5-30 min) were evaluated, and their effect was assessed by enzymatic hydrolysis with 2 g of Celluclast + Ultraflo mixture/ 100 g of starch-free fiber (SFF) slurry at 5% dry matter (DM). The highest overall sugar yield for the combined steam pretreatment and enzymatic hydrolysis, 52g/100g of DM of SFF, corresponding to 74% of the theoretical, was achieved with pretreatment at 190degreesC for 10 min followed by enzymatic hydrolysis. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied Biochemistry and Biotechnology
volume
115
issue
1-3
pages
989 - 1002
publisher
Humana Press
external identifiers
  • wos:000221186200081
ISSN
1559-0291
DOI
10.1385/ABAB:115:1-3:0989
language
English
LU publication?
yes
id
b707f7f5-5261-40f7-8d3c-e74acda7ff19 (old id 138866)
date added to LUP
2007-06-25 14:31:00
date last changed
2016-04-15 19:28:03
@article{b707f7f5-5261-40f7-8d3c-e74acda7ff19,
  abstract     = {Steam treatment of an industrial process stream, denoted starch-free wheat fiber, was investigated to improve the formation of monomeric sugars in subsequent enzymatic hydrolysis for further bioconversion into ethanol. The solid fraction in the process stream, derived from a combined starch and ethanol factory, was rich in arabinose (21.1%), xylose (30.1%), and glucose (18.6%), in the form of polysaccharides. Various conditions of steam pretreatment (170-220degreesC for 5-30 min) were evaluated, and their effect was assessed by enzymatic hydrolysis with 2 g of Celluclast + Ultraflo mixture/ 100 g of starch-free fiber (SFF) slurry at 5% dry matter (DM). The highest overall sugar yield for the combined steam pretreatment and enzymatic hydrolysis, 52g/100g of DM of SFF, corresponding to 74% of the theoretical, was achieved with pretreatment at 190degreesC for 10 min followed by enzymatic hydrolysis.},
  author       = {Palmarola Adrados, Beatriz and Galbe, Mats and Zacchi, Guido},
  issn         = {1559-0291},
  language     = {eng},
  number       = {1-3},
  pages        = {989--1002},
  publisher    = {Humana Press},
  series       = {Applied Biochemistry and Biotechnology},
  title        = {Combined steam pretreatment and enzymatic hydrolysis of starch-free wheat fibers},
  url          = {http://dx.doi.org/10.1385/ABAB:115:1-3:0989},
  volume       = {115},
  year         = {2004},
}