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Hydrolysis of nonstarch carbohydrates of wheat-starch effluent for ethanol production

Palmarola Adrados, Beatriz LU ; Juhasz, T; Galbe, Mats LU and Zacchi, Guido LU (2004) In Biotechnology Progress 20(2). p.474-479
Abstract
A (polysaccharide-rich) waste stream derived from a combined starch and ethanol factory was investigated regarding hydrolysis of the nonstarch carbohydrates for ethanol production. The material was characterized and processed to yield the maximum amount of sugars. The starch fraction was hydrolyzed with amylolytic enzymes, and the resulting fibrous material was separated by filtration. This material, denoted starch-free fibers (SFF), was subjected to heat treatment followed by enzymatic hydrolysis to recover the other major carbohydrate components, namely, cellulose and hemicellulose, in monomeric form. Heat treatment in a microwave oven efficiently solubilized a fraction of these polysaccharides and made the material more accessible to... (More)
A (polysaccharide-rich) waste stream derived from a combined starch and ethanol factory was investigated regarding hydrolysis of the nonstarch carbohydrates for ethanol production. The material was characterized and processed to yield the maximum amount of sugars. The starch fraction was hydrolyzed with amylolytic enzymes, and the resulting fibrous material was separated by filtration. This material, denoted starch-free fibers (SFF), was subjected to heat treatment followed by enzymatic hydrolysis to recover the other major carbohydrate components, namely, cellulose and hemicellulose, in monomeric form. Heat treatment in a microwave oven efficiently solubilized a fraction of these polysaccharides and made the material more accessible to the cellulolytic and hemicellulolytic enzymes used in the subsequent enzymatic hydrolysis. The maximum sugar yield after enzymatic hydrolysis, achieved with pretreatment at 170 degreesC for 40 min, was 34.1 g per 100 g SFF, comprising 12.8 g glucose, 13.9 g xylose and 7.4 g arabinose, corresponding to 66%, 71% and 51% of the theoretical, respectively. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biotechnology Progress
volume
20
issue
2
pages
474 - 479
publisher
The American Chemical Society
external identifiers
  • wos:000220652600009
  • scopus:11144357448
ISSN
1520-6033
DOI
10.1021/bp034243h
language
English
LU publication?
yes
id
9f028d2e-8d16-4cd7-9167-8f126a47854b (old id 138875)
date added to LUP
2007-06-25 14:28:17
date last changed
2017-03-19 04:15:24
@article{9f028d2e-8d16-4cd7-9167-8f126a47854b,
  abstract     = {A (polysaccharide-rich) waste stream derived from a combined starch and ethanol factory was investigated regarding hydrolysis of the nonstarch carbohydrates for ethanol production. The material was characterized and processed to yield the maximum amount of sugars. The starch fraction was hydrolyzed with amylolytic enzymes, and the resulting fibrous material was separated by filtration. This material, denoted starch-free fibers (SFF), was subjected to heat treatment followed by enzymatic hydrolysis to recover the other major carbohydrate components, namely, cellulose and hemicellulose, in monomeric form. Heat treatment in a microwave oven efficiently solubilized a fraction of these polysaccharides and made the material more accessible to the cellulolytic and hemicellulolytic enzymes used in the subsequent enzymatic hydrolysis. The maximum sugar yield after enzymatic hydrolysis, achieved with pretreatment at 170 degreesC for 40 min, was 34.1 g per 100 g SFF, comprising 12.8 g glucose, 13.9 g xylose and 7.4 g arabinose, corresponding to 66%, 71% and 51% of the theoretical, respectively.},
  author       = {Palmarola Adrados, Beatriz and Juhasz, T and Galbe, Mats and Zacchi, Guido},
  issn         = {1520-6033},
  language     = {eng},
  number       = {2},
  pages        = {474--479},
  publisher    = {The American Chemical Society},
  series       = {Biotechnology Progress},
  title        = {Hydrolysis of nonstarch carbohydrates of wheat-starch effluent for ethanol production},
  url          = {http://dx.doi.org/10.1021/bp034243h},
  volume       = {20},
  year         = {2004},
}