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Use of microfiltration as first step in recovery of protein A from fermentation broth

Persson, Anna LU ; Jönsson, Ann-Sofi LU and Zacchi, Guido LU (2004) In Applied Biochemistry and Biotechnology 112(3). p.151-162
Abstract
The flux and transmission of protein A during microfiltration have been studied. We studied the performance of two commercial membranes: one made of nylon (Pall Ultipore Nylon(66), 0.2 mum) and one of polyether sulfone (Pall Omega, 0.16 mum). The Nylon(66) membrane had by far the best transmission of protein A although a previous study showed that bovine serum albumin (BSA), often used to characterize membranes, had much better transmission through the Omega membrane. The membrane manufacturer also states that the Omega membrane is the best membrane for this kind of application because it is a low-protein-binding membrane. The lower transmission of the Omega membrane for protein A was assumed to be owing to its smaller pores and higher... (More)
The flux and transmission of protein A during microfiltration have been studied. We studied the performance of two commercial membranes: one made of nylon (Pall Ultipore Nylon(66), 0.2 mum) and one of polyether sulfone (Pall Omega, 0.16 mum). The Nylon(66) membrane had by far the best transmission of protein A although a previous study showed that bovine serum albumin (BSA), often used to characterize membranes, had much better transmission through the Omega membrane. The membrane manufacturer also states that the Omega membrane is the best membrane for this kind of application because it is a low-protein-binding membrane. The lower transmission of the Omega membrane for protein A was assumed to be owing to its smaller pores and higher charge density in combination with the larger Stokes radius for protein A. When the pH was lowered, the Nylon(66) membrane still had the higher transmission. It can thus be concluded that a membrane that is found suitable for the recovery process of one protein is not always the best choice for the recovery process for other proteins even though the membrane is low protein binding. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied Biochemistry and Biotechnology
volume
112
issue
3
pages
151 - 162
publisher
Humana Press
external identifiers
  • wos:000220409100004
  • pmid:15007183
  • scopus:11244287147
ISSN
1559-0291
DOI
10.1385/ABAB:112:3:151
language
English
LU publication?
yes
id
75aaf5e6-93c2-4391-95b8-0877a07efda2 (old id 138891)
date added to LUP
2007-06-25 14:53:43
date last changed
2017-08-27 03:47:48
@article{75aaf5e6-93c2-4391-95b8-0877a07efda2,
  abstract     = {The flux and transmission of protein A during microfiltration have been studied. We studied the performance of two commercial membranes: one made of nylon (Pall Ultipore Nylon(66), 0.2 mum) and one of polyether sulfone (Pall Omega, 0.16 mum). The Nylon(66) membrane had by far the best transmission of protein A although a previous study showed that bovine serum albumin (BSA), often used to characterize membranes, had much better transmission through the Omega membrane. The membrane manufacturer also states that the Omega membrane is the best membrane for this kind of application because it is a low-protein-binding membrane. The lower transmission of the Omega membrane for protein A was assumed to be owing to its smaller pores and higher charge density in combination with the larger Stokes radius for protein A. When the pH was lowered, the Nylon(66) membrane still had the higher transmission. It can thus be concluded that a membrane that is found suitable for the recovery process of one protein is not always the best choice for the recovery process for other proteins even though the membrane is low protein binding.},
  author       = {Persson, Anna and Jönsson, Ann-Sofi and Zacchi, Guido},
  issn         = {1559-0291},
  language     = {eng},
  number       = {3},
  pages        = {151--162},
  publisher    = {Humana Press},
  series       = {Applied Biochemistry and Biotechnology},
  title        = {Use of microfiltration as first step in recovery of protein A from fermentation broth},
  url          = {http://dx.doi.org/10.1385/ABAB:112:3:151},
  volume       = {112},
  year         = {2004},
}