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Patch testing of patients allergic to Disperse Blue 106 and Disperse Blue 124 with thin-layer chromatograms and purified dyes.

Ryberg, Kristina LU ; Goossens, An ; Isaksson, Marléne LU ; Gruvberger, Birgitta LU ; Zimerson, Erik LU ; Persson, Lena and Bruze, Magnus LU (2009) In Contact Dermatitis 60(5). p.270-278
Abstract
BACKGROUND: Former investigations have demonstrated that patch test preparations containing Disperse Blue (DB) 106 and DB 124 are impure. OBJECTIVE: To investigate the significance of impurities in preparations of disperse dyes with regard to contact allergy. MATERIALS/METHODS: 21 patients allergic to DB 106 and/or DB 124 were tested with dilution series of commercial and purified DB 106 and DB 124. All patients were additionally tested with thin-layer chromatography (TLC) strips made from the commercial preparations containing DB 106 and 18 of them also with TLC strips containing DB 124. RESULTS: 13 of 21 and 12/18 patients tested positively to the strips of DB 106 and DB 124, respectively. Among these patients, 4/13 and 5/12,... (More)
BACKGROUND: Former investigations have demonstrated that patch test preparations containing Disperse Blue (DB) 106 and DB 124 are impure. OBJECTIVE: To investigate the significance of impurities in preparations of disperse dyes with regard to contact allergy. MATERIALS/METHODS: 21 patients allergic to DB 106 and/or DB 124 were tested with dilution series of commercial and purified DB 106 and DB 124. All patients were additionally tested with thin-layer chromatography (TLC) strips made from the commercial preparations containing DB 106 and 18 of them also with TLC strips containing DB 124. RESULTS: 13 of 21 and 12/18 patients tested positively to the strips of DB 106 and DB 124, respectively. Among these patients, 4/13 and 5/12, respectively, did not react to the main spot but reacted to other spots. Dilution series of purified DB 106 and DB 124 were positive in 10 patients each, while 16 and 15 patients, respectively, tested positively to the dilution series of the corresponding commercial disperse dye. CONCLUSION: Approximately 25% of the patients, diagnosed as contact allergic to DB 106 and DB 124, only reacted to impurities in the patch test preparations. This has implications for diagnosis and prevention. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Contact Dermatitis
volume
60
issue
5
pages
270 - 278
publisher
Wiley-Blackwell
external identifiers
  • wos:000265481000004
  • pmid:19397619
  • scopus:65349089445
  • pmid:19397619
ISSN
0105-1873
DOI
10.1111/j.1600-0536.2009.01538.x
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Drug Target Discovery (013212045), Occupational and Environmental Dermatology Unit (013241310), Pediatrics/Urology/Gynecology/Endocrinology (013240400)
id
2bd95aa1-01ca-45d7-8496-942b1a309573 (old id 1391804)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19397619?dopt=Abstract
date added to LUP
2016-04-04 08:47:57
date last changed
2025-04-04 15:06:21
@article{2bd95aa1-01ca-45d7-8496-942b1a309573,
  abstract     = {{BACKGROUND: Former investigations have demonstrated that patch test preparations containing Disperse Blue (DB) 106 and DB 124 are impure. OBJECTIVE: To investigate the significance of impurities in preparations of disperse dyes with regard to contact allergy. MATERIALS/METHODS: 21 patients allergic to DB 106 and/or DB 124 were tested with dilution series of commercial and purified DB 106 and DB 124. All patients were additionally tested with thin-layer chromatography (TLC) strips made from the commercial preparations containing DB 106 and 18 of them also with TLC strips containing DB 124. RESULTS: 13 of 21 and 12/18 patients tested positively to the strips of DB 106 and DB 124, respectively. Among these patients, 4/13 and 5/12, respectively, did not react to the main spot but reacted to other spots. Dilution series of purified DB 106 and DB 124 were positive in 10 patients each, while 16 and 15 patients, respectively, tested positively to the dilution series of the corresponding commercial disperse dye. CONCLUSION: Approximately 25% of the patients, diagnosed as contact allergic to DB 106 and DB 124, only reacted to impurities in the patch test preparations. This has implications for diagnosis and prevention.}},
  author       = {{Ryberg, Kristina and Goossens, An and Isaksson, Marléne and Gruvberger, Birgitta and Zimerson, Erik and Persson, Lena and Bruze, Magnus}},
  issn         = {{0105-1873}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{270--278}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Contact Dermatitis}},
  title        = {{Patch testing of patients allergic to Disperse Blue 106 and Disperse Blue 124 with thin-layer chromatograms and purified dyes.}},
  url          = {{http://dx.doi.org/10.1111/j.1600-0536.2009.01538.x}},
  doi          = {{10.1111/j.1600-0536.2009.01538.x}},
  volume       = {{60}},
  year         = {{2009}},
}