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Polarization Portraits of Single Multichromophoric Systems: Visualizing Conformation and Energy Transfer.

Mirzov, Oleg LU ; Bloem, Robbert LU ; Hania, Ralph LU ; Thomsson, Daniel LU ; Lin, Hongzhen LU and Scheblykin, Ivan LU (2009) In Small 5. p.1877-1888
Abstract
A novel technique, two-dimensional (2D) polarization single-molecule imaging, is presented. It is based on measurements and analysis of fluorescence intensity as a function of excitation and emission polarization angles. The technique allows recording of full information on the steady-state polarization properties of fluorescent objects. It is particularly suitable for application to single multichromophoric systems (molecules or nanoparticles) with energy transfer (ET) between different chromophores (e.g., single fluorescent pi-conjugated polymer chains). The 2D polarization data simultaneously provide information on the conformation of the system and the efficiency of its internal excitation ET. The technique is used to characterize... (More)
A novel technique, two-dimensional (2D) polarization single-molecule imaging, is presented. It is based on measurements and analysis of fluorescence intensity as a function of excitation and emission polarization angles. The technique allows recording of full information on the steady-state polarization properties of fluorescent objects. It is particularly suitable for application to single multichromophoric systems (molecules or nanoparticles) with energy transfer (ET) between different chromophores (e.g., single fluorescent pi-conjugated polymer chains). The 2D polarization data simultaneously provide information on the conformation of the system and the efficiency of its internal excitation ET. The technique is used to characterize single chains and different kinds of chain aggregates of different conjugated polymers at different temperatures. The 2D polarization measurements reveal a dramatic difference in ET taking place in these systems. Clear temperature dependence of ET is observed for individual aggregates as well as for their statistical ensembles. Also, a dependence on solvent and aggregate size is shown. Additionally, extensive "traditional one-dimensional" polarization results on the polarization anisotropy of fluorescence excitation and emission are presented. These results and findings are discussed in relation to internal organization of the nano-objects under study. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Small
volume
5
pages
1877 - 1888
publisher
John Wiley & Sons
external identifiers
  • wos:000269227800012
  • pmid:19384881
  • scopus:69049095136
ISSN
1613-6829
DOI
10.1002/smll.200801168
language
English
LU publication?
yes
id
ee14dcb4-a995-4cc4-8cf4-81a37c2abc98 (old id 1391919)
date added to LUP
2009-05-12 09:56:31
date last changed
2017-12-10 03:46:51
@article{ee14dcb4-a995-4cc4-8cf4-81a37c2abc98,
  abstract     = {A novel technique, two-dimensional (2D) polarization single-molecule imaging, is presented. It is based on measurements and analysis of fluorescence intensity as a function of excitation and emission polarization angles. The technique allows recording of full information on the steady-state polarization properties of fluorescent objects. It is particularly suitable for application to single multichromophoric systems (molecules or nanoparticles) with energy transfer (ET) between different chromophores (e.g., single fluorescent pi-conjugated polymer chains). The 2D polarization data simultaneously provide information on the conformation of the system and the efficiency of its internal excitation ET. The technique is used to characterize single chains and different kinds of chain aggregates of different conjugated polymers at different temperatures. The 2D polarization measurements reveal a dramatic difference in ET taking place in these systems. Clear temperature dependence of ET is observed for individual aggregates as well as for their statistical ensembles. Also, a dependence on solvent and aggregate size is shown. Additionally, extensive "traditional one-dimensional" polarization results on the polarization anisotropy of fluorescence excitation and emission are presented. These results and findings are discussed in relation to internal organization of the nano-objects under study.},
  author       = {Mirzov, Oleg and Bloem, Robbert and Hania, Ralph and Thomsson, Daniel and Lin, Hongzhen and Scheblykin, Ivan},
  issn         = {1613-6829},
  language     = {eng},
  pages        = {1877--1888},
  publisher    = {John Wiley & Sons},
  series       = {Small},
  title        = {Polarization Portraits of Single Multichromophoric Systems: Visualizing Conformation and Energy Transfer.},
  url          = {http://dx.doi.org/10.1002/smll.200801168},
  volume       = {5},
  year         = {2009},
}