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Supported lipid bilayers, tethered lipid vesicles, and vesicle fusion investigated using gravimetric, plasmonic, and microscopy techniques

Höök, Fredrik LU ; Stengel, Gudrun LU ; Dahlin, Andreas LU ; Gunnarsson, Anders LU ; Jonsson, Magnus LU ; Jönsson, Peter LU ; Reimhult, Erik; Simonsson, Lisa LU and Svedhem, Sofia (2008) International Workshop on Novel Model Systems for Bimolecular Lipid Membranes In Biointerphases 3(2). p.108-116
Abstract
This article summarizes our most recent contributions to the rapidly growing field of supported lipid assemblies with emphasis on current studies addressing both fundamental and applied aspects of supported lipid bilayer (SLB) and tethered lipid vesicles (TLVs) to be utilized in sensing applications. The new insights obtained from combining the quartz crystal microbalance with dissipation monitoring technique with surface plasmon resonance are described, and we also present recent studies in which nanoplasmonic sensing has been used in studies of SLBs and TLVs. To gain full control over the spatial arrangement of TLVs in both two and three dimensions, we have developed a method for site-selective and sequence-specific sorting of DNA-tagged... (More)
This article summarizes our most recent contributions to the rapidly growing field of supported lipid assemblies with emphasis on current studies addressing both fundamental and applied aspects of supported lipid bilayer (SLB) and tethered lipid vesicles (TLVs) to be utilized in sensing applications. The new insights obtained from combining the quartz crystal microbalance with dissipation monitoring technique with surface plasmon resonance are described, and we also present recent studies in which nanoplasmonic sensing has been used in studies of SLBs and TLVs. To gain full control over the spatial arrangement of TLVs in both two and three dimensions, we have developed a method for site-selective and sequence-specific sorting of DNA-tagged vesicles to surfaces modified with complementary DNA. The combination of this method with nanoplasmonic sensing formats is covered as well as the possibility of using DNA-modified vesicles for the detection of unlabeled DNA targets on the single-molecule level. Finally, a new method for membrane fusion induced by hybridization of vesicle-anchored DNA is demonstrated, including new results on content mixing obtained with vesicle populations encapsulating short, complementary DNA strands. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
plasmonics, lipid bilayers, biomembranes, DNA, surface plasmon resonance
in
Biointerphases
volume
3
issue
2
pages
108 - 116
publisher
American Vacuum Society
conference name
International Workshop on Novel Model Systems for Bimolecular Lipid Membranes
external identifiers
  • wos:000264979100017
  • scopus:70349106129
ISSN
1934-8630
1559-4106
DOI
10.1116/1.2948313
language
English
LU publication?
yes
id
61058e05-9cc2-4979-b041-ec4fcb974d27 (old id 1399300)
date added to LUP
2009-05-28 13:44:30
date last changed
2017-02-12 03:29:50
@inproceedings{61058e05-9cc2-4979-b041-ec4fcb974d27,
  abstract     = {This article summarizes our most recent contributions to the rapidly growing field of supported lipid assemblies with emphasis on current studies addressing both fundamental and applied aspects of supported lipid bilayer (SLB) and tethered lipid vesicles (TLVs) to be utilized in sensing applications. The new insights obtained from combining the quartz crystal microbalance with dissipation monitoring technique with surface plasmon resonance are described, and we also present recent studies in which nanoplasmonic sensing has been used in studies of SLBs and TLVs. To gain full control over the spatial arrangement of TLVs in both two and three dimensions, we have developed a method for site-selective and sequence-specific sorting of DNA-tagged vesicles to surfaces modified with complementary DNA. The combination of this method with nanoplasmonic sensing formats is covered as well as the possibility of using DNA-modified vesicles for the detection of unlabeled DNA targets on the single-molecule level. Finally, a new method for membrane fusion induced by hybridization of vesicle-anchored DNA is demonstrated, including new results on content mixing obtained with vesicle populations encapsulating short, complementary DNA strands.},
  author       = {Höök, Fredrik and Stengel, Gudrun and Dahlin, Andreas and Gunnarsson, Anders and Jonsson, Magnus and Jönsson, Peter and Reimhult, Erik and Simonsson, Lisa and Svedhem, Sofia},
  booktitle    = {Biointerphases},
  issn         = {1934-8630},
  keyword      = {plasmonics,lipid bilayers,biomembranes,DNA,surface plasmon resonance},
  language     = {eng},
  number       = {2},
  pages        = {108--116},
  publisher    = {American Vacuum Society},
  title        = {Supported lipid bilayers, tethered lipid vesicles, and vesicle fusion investigated using gravimetric, plasmonic, and microscopy techniques},
  url          = {http://dx.doi.org/10.1116/1.2948313},
  volume       = {3},
  year         = {2008},
}