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Report of the Third International Workshop on Molecular Blood Group Genotyping

Daniels, G.; van der Schoot, C. E.; Gassner, C. and Olsson, Martin L LU (2009) In Vox Sanguinis 96(4). p.337-343
Abstract
The Third International Society of Blood Transfusion Workshop on Molecular Blood Group Genotyping was held in 2008, with a feedback meeting at the International Society of Blood Transfusion Congress in Macao SAR, China. Thirty-three laboratories participated, eight less than in 2006. Six samples were distributed: sample 1 representing DNA from a sample referred because of abnormal serological results in D testing; samples 2 and 3 from transfusion-dependent patients for testing for all clinically important polymorphisms; sample 4 a mixture of two DNA samples designed to simulate a chimera, referred because of abnormal serological results in donor testing; and samples 5 and 6 plasma samples from RhD-negative pregnant women, for fetal RhD... (More)
The Third International Society of Blood Transfusion Workshop on Molecular Blood Group Genotyping was held in 2008, with a feedback meeting at the International Society of Blood Transfusion Congress in Macao SAR, China. Thirty-three laboratories participated, eight less than in 2006. Six samples were distributed: sample 1 representing DNA from a sample referred because of abnormal serological results in D testing; samples 2 and 3 from transfusion-dependent patients for testing for all clinically important polymorphisms; sample 4 a mixture of two DNA samples designed to simulate a chimera, referred because of abnormal serological results in donor testing; and samples 5 and 6 plasma samples from RhD-negative pregnant women, for fetal RhD testing (only tested by 17 laboratories). For samples 1-3, 24 of 33 laboratories obtained completely correct results. For sample 4, the ability to detect the minority DNA population was partly dependent on method. Of the 17 laboratories that received samples 5 and 6, 13 reported correct results on both samples. Overall a small improvement from previous workshops was noted, but there is still room for improvement. The main conclusion for the 2006 workshop can be reiterated: with greater care and attention to detail, very high standards could be set for molecular blood group genotyping. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Rh, molecular genetics, blood groups, fetal genotyping
in
Vox Sanguinis
volume
96
issue
4
pages
337 - 343
publisher
Federation of European Neuroscience Societies and Blackwell Publishing Ltd
external identifiers
  • wos:000265190000010
  • scopus:59649096216
ISSN
1423-0410
DOI
10.1111/j.1423-0410.2009.01165.x
language
English
LU publication?
yes
id
c63c770c-d2ed-4b08-adf6-0cdc7156d76f (old id 1399659)
date added to LUP
2009-06-15 15:06:29
date last changed
2017-01-01 05:58:28
@article{c63c770c-d2ed-4b08-adf6-0cdc7156d76f,
  abstract     = {The Third International Society of Blood Transfusion Workshop on Molecular Blood Group Genotyping was held in 2008, with a feedback meeting at the International Society of Blood Transfusion Congress in Macao SAR, China. Thirty-three laboratories participated, eight less than in 2006. Six samples were distributed: sample 1 representing DNA from a sample referred because of abnormal serological results in D testing; samples 2 and 3 from transfusion-dependent patients for testing for all clinically important polymorphisms; sample 4 a mixture of two DNA samples designed to simulate a chimera, referred because of abnormal serological results in donor testing; and samples 5 and 6 plasma samples from RhD-negative pregnant women, for fetal RhD testing (only tested by 17 laboratories). For samples 1-3, 24 of 33 laboratories obtained completely correct results. For sample 4, the ability to detect the minority DNA population was partly dependent on method. Of the 17 laboratories that received samples 5 and 6, 13 reported correct results on both samples. Overall a small improvement from previous workshops was noted, but there is still room for improvement. The main conclusion for the 2006 workshop can be reiterated: with greater care and attention to detail, very high standards could be set for molecular blood group genotyping.},
  author       = {Daniels, G. and van der Schoot, C. E. and Gassner, C. and Olsson, Martin L},
  issn         = {1423-0410},
  keyword      = {Rh,molecular genetics,blood groups,fetal genotyping},
  language     = {eng},
  number       = {4},
  pages        = {337--343},
  publisher    = {Federation of European Neuroscience Societies and Blackwell Publishing Ltd},
  series       = {Vox Sanguinis},
  title        = {Report of the Third International Workshop on Molecular Blood Group Genotyping},
  url          = {http://dx.doi.org/10.1111/j.1423-0410.2009.01165.x},
  volume       = {96},
  year         = {2009},
}