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Biofilm formation by Staphylococcus epidermidis on peritoneal dialysis catheters and the effects of extracellular products from Pseudomonas aeruginosa.

Pihl, Maria; Arvidsson, Anna; Skepö, Marie; Nilsson, Martin LU ; Givskov, Michael; Tolker-Nielsen, Tim; Svensäter, Gunnel and Davies, Julia R (2013) In Pathogens and Disease 67(3). p.192-198
Abstract
Biofilm formation by Staphylococcus epidermidis is a cause of infections related to peritoneal dialysis (PD). We have used a PD catheter flow-cell model in combination with confocal scanning laser microscopy and atomic force microscopy to study biofilm formation by S. epidermidis. Adherence to serum-coated catheters was four times greater than to uncoated ones, suggesting that S. epidermidis binds to serum proteins on the catheter surface. Pseudomonas aeruginosa biofilm supernatant interfered with the formation of a serum protein coat thereby reducing the capacity for biofilm formation in S. epidermidis. Supernatants from ΔpelA, ΔpslBCD and ΔrhlAB strains of P. aeruginosa showed no differences from the wild-type supernatant indicating that... (More)
Biofilm formation by Staphylococcus epidermidis is a cause of infections related to peritoneal dialysis (PD). We have used a PD catheter flow-cell model in combination with confocal scanning laser microscopy and atomic force microscopy to study biofilm formation by S. epidermidis. Adherence to serum-coated catheters was four times greater than to uncoated ones, suggesting that S. epidermidis binds to serum proteins on the catheter surface. Pseudomonas aeruginosa biofilm supernatant interfered with the formation of a serum protein coat thereby reducing the capacity for biofilm formation in S. epidermidis. Supernatants from ΔpelA, ΔpslBCD and ΔrhlAB strains of P. aeruginosa showed no differences from the wild-type supernatant indicating that the effect on serum coat formation was not due to rhamnolipids or the PelA and PslBCD polysaccharides. Supernatant from P. aeruginosa also dispersed established S. epidermidis biofilms. Supernatants lacking PelA or PslBCD showed no differences from the wild type but that from a ΔrhlAB strain, showed reduced, but not abolished, capacity for dispersal. This suggests that rhamnolipids are involved but not wholly responsible for the effect. Thus, supernatants from P. aeruginosa contain promising substances for the prevention and treatment of biofilm infections, although further work is required to identity more active components. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Pathogens and Disease
volume
67
issue
3
pages
192 - 198
publisher
Wiley-Blackwell
external identifiers
  • wos:000318349200004
  • pmid:23620182
  • scopus:84883747908
ISSN
2049-632X
DOI
10.1111/2049-632X.12035
language
English
LU publication?
yes
id
139cba99-b79b-4e7c-bcfd-97130a0d9b4a (old id 3733320)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/23620182?dopt=Abstract
date added to LUP
2013-05-04 21:30:17
date last changed
2019-10-01 01:05:55
@article{139cba99-b79b-4e7c-bcfd-97130a0d9b4a,
  abstract     = {Biofilm formation by Staphylococcus epidermidis is a cause of infections related to peritoneal dialysis (PD). We have used a PD catheter flow-cell model in combination with confocal scanning laser microscopy and atomic force microscopy to study biofilm formation by S. epidermidis. Adherence to serum-coated catheters was four times greater than to uncoated ones, suggesting that S. epidermidis binds to serum proteins on the catheter surface. Pseudomonas aeruginosa biofilm supernatant interfered with the formation of a serum protein coat thereby reducing the capacity for biofilm formation in S. epidermidis. Supernatants from ΔpelA, ΔpslBCD and ΔrhlAB strains of P. aeruginosa showed no differences from the wild-type supernatant indicating that the effect on serum coat formation was not due to rhamnolipids or the PelA and PslBCD polysaccharides. Supernatant from P. aeruginosa also dispersed established S. epidermidis biofilms. Supernatants lacking PelA or PslBCD showed no differences from the wild type but that from a ΔrhlAB strain, showed reduced, but not abolished, capacity for dispersal. This suggests that rhamnolipids are involved but not wholly responsible for the effect. Thus, supernatants from P. aeruginosa contain promising substances for the prevention and treatment of biofilm infections, although further work is required to identity more active components.},
  author       = {Pihl, Maria and Arvidsson, Anna and Skepö, Marie and Nilsson, Martin and Givskov, Michael and Tolker-Nielsen, Tim and Svensäter, Gunnel and Davies, Julia R},
  issn         = {2049-632X},
  language     = {eng},
  number       = {3},
  pages        = {192--198},
  publisher    = {Wiley-Blackwell},
  series       = {Pathogens and Disease},
  title        = {Biofilm formation by Staphylococcus epidermidis on peritoneal dialysis catheters and the effects of extracellular products from Pseudomonas aeruginosa.},
  url          = {http://dx.doi.org/10.1111/2049-632X.12035},
  volume       = {67},
  year         = {2013},
}