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Topical Haemostatics in Renal Trauma-An Evaluation of Four Different Substances in an Experimental Setting

Björses, Katarina LU and Holst, Jan LU (2009) Meeting of the Swedish-Surgical-Society In Journal of Trauma 66(3). p.602-611
Abstract
BACKGROUND: Damage control is valuable in hemodynamically unstable trauma patients. To improve the hemostasis of packing, topical hemostatic agents have been suggested. The effects of such agents are unclear in trauma situations. The purpose of this study was to investigate the hemostatic capacity, and the stability of the hemostatic clot, of four substances with different mode of action in an experimental traumatic bleeding model. METHODS: A standardized heminefrectomy was performed in 180 heparinized and normotensive Sprauge-Dawley rats. Four different substances were studied (separately and in combinations) in a randomized fashion: gelatin (sponge and matrix), bovine thrombin, freeze-dried recombinant factor VIIa (rFVIIa), and... (More)
BACKGROUND: Damage control is valuable in hemodynamically unstable trauma patients. To improve the hemostasis of packing, topical hemostatic agents have been suggested. The effects of such agents are unclear in trauma situations. The purpose of this study was to investigate the hemostatic capacity, and the stability of the hemostatic clot, of four substances with different mode of action in an experimental traumatic bleeding model. METHODS: A standardized heminefrectomy was performed in 180 heparinized and normotensive Sprauge-Dawley rats. Four different substances were studied (separately and in combinations) in a randomized fashion: gelatin (sponge and matrix), bovine thrombin, freeze-dried recombinant factor VIIa (rFVIIa), and microporous polysaccharide hemospheres. Eight treatment groups (15 animals/group) were considered, primary endpoint was hemostasis within 20 minutes of observation. The effective treatment groups were evaluated in a second set in the same experimental model, but with a prolonged observation time after hemostasis (60 minutes) to control the stability of the clot. RESULTS: Those animals treated with gelatin in the comparative study, with and without thrombin or rFVIIa, obtained hemostasis. Thrombin and rFVIIa alone did not have any hemostatic capacity. Only 20% to 25% of the animals obtained hemostasis with microporous polysaccharide hemospheres alone or in combination with rFVIIa. In the prolonged observation study, gelatin alone and in combination with thrombin or rFVIIa was studied. On average, 34% (20%-54%) of the animals rebled with no significant difference between the treatment groups. CONCLUSIONS: Gelatin-containing products provided a fast hemostasis in this experimental model. One third of the animals rebled, regardless of whether thrombin or rFVIIa was added. Further studies are demanded to confirm these results clinically. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Kidney: injuries, Hemorrhage: drug therapy, Hemostatics: administration & dosage
in
Journal of Trauma
volume
66
issue
3
pages
602 - 611
publisher
Lippincott Williams and Wilkins
conference name
Meeting of the Swedish-Surgical-Society
external identifiers
  • WOS:000264259000003
  • PMID:19276727
  • Scopus:68149157764
ISSN
0022-5282
DOI
10.1097/TA.0b013e3181823533
language
English
LU publication?
yes
id
a1b6935c-1bc6-428c-89d4-acb84e3b32d5 (old id 1400576)
date added to LUP
2009-04-07 16:22:04
date last changed
2017-01-01 08:27:48
@article{a1b6935c-1bc6-428c-89d4-acb84e3b32d5,
  abstract     = {BACKGROUND: Damage control is valuable in hemodynamically unstable trauma patients. To improve the hemostasis of packing, topical hemostatic agents have been suggested. The effects of such agents are unclear in trauma situations. The purpose of this study was to investigate the hemostatic capacity, and the stability of the hemostatic clot, of four substances with different mode of action in an experimental traumatic bleeding model. METHODS: A standardized heminefrectomy was performed in 180 heparinized and normotensive Sprauge-Dawley rats. Four different substances were studied (separately and in combinations) in a randomized fashion: gelatin (sponge and matrix), bovine thrombin, freeze-dried recombinant factor VIIa (rFVIIa), and microporous polysaccharide hemospheres. Eight treatment groups (15 animals/group) were considered, primary endpoint was hemostasis within 20 minutes of observation. The effective treatment groups were evaluated in a second set in the same experimental model, but with a prolonged observation time after hemostasis (60 minutes) to control the stability of the clot. RESULTS: Those animals treated with gelatin in the comparative study, with and without thrombin or rFVIIa, obtained hemostasis. Thrombin and rFVIIa alone did not have any hemostatic capacity. Only 20% to 25% of the animals obtained hemostasis with microporous polysaccharide hemospheres alone or in combination with rFVIIa. In the prolonged observation study, gelatin alone and in combination with thrombin or rFVIIa was studied. On average, 34% (20%-54%) of the animals rebled with no significant difference between the treatment groups. CONCLUSIONS: Gelatin-containing products provided a fast hemostasis in this experimental model. One third of the animals rebled, regardless of whether thrombin or rFVIIa was added. Further studies are demanded to confirm these results clinically.},
  author       = {Björses, Katarina and Holst, Jan},
  issn         = {0022-5282},
  keyword      = {Kidney: injuries,Hemorrhage: drug therapy,Hemostatics: administration & dosage},
  language     = {eng},
  number       = {3},
  pages        = {602--611},
  publisher    = {Lippincott Williams and Wilkins},
  series       = {Journal of Trauma},
  title        = {Topical Haemostatics in Renal Trauma-An Evaluation of Four Different Substances in an Experimental Setting},
  url          = {http://dx.doi.org/10.1097/TA.0b013e3181823533},
  volume       = {66},
  year         = {2009},
}