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Levels Of Bacterial Endotoxin In The Samples Of Settled Dust Collected In Animal Houses

Pomorska, Dorota; Larsson, Lennart LU ; Skorska, Czeslawa; Sitkowska, Jolanta and Dutkiewicz, Jacek (2009) In Bulletin of the Veterinary Institute in Puławy 53(1). p.37-41
Abstract
Samples of settled dust were collected in 14 animal houses and examined for the presence of bacterial endotoxin with the Limulus (LAL) test and the gas chromatography - tandem mass spectrometry (GC-MSMS) technique, based oil the detection of 3-hydroxy fatty acids (3-OH-FAs) as chemical markers of the endotoxin lipopolysaccharide. The median concentrations of the endotoxin in dust determined with LAL test in sheep sheds, poultry houses, and horse stables were 15,687.5 mu g/g, 8,081.8 mu g/g, and 79.3 mu g/g, respectively, while those determined with the GC-MSMS technique were 868.0 mu g/g, 580.0 mu g/g, and 496.0 mu g/g, respectively. Statistical comparison of the results yielded with LAL test and GC-MSMS technique revealed a weak... (More)
Samples of settled dust were collected in 14 animal houses and examined for the presence of bacterial endotoxin with the Limulus (LAL) test and the gas chromatography - tandem mass spectrometry (GC-MSMS) technique, based oil the detection of 3-hydroxy fatty acids (3-OH-FAs) as chemical markers of the endotoxin lipopolysaccharide. The median concentrations of the endotoxin in dust determined with LAL test in sheep sheds, poultry houses, and horse stables were 15,687.5 mu g/g, 8,081.8 mu g/g, and 79.3 mu g/g, respectively, while those determined with the GC-MSMS technique were 868.0 mu g/g, 580.0 mu g/g, and 496.0 mu g/g, respectively. Statistical comparison of the results yielded with LAL test and GC-MSMS technique revealed a weak correlation between both methods. Fatty acids with 14-16 carbon chains (3-OH-C-14 and 3-OH-C-16) were predominant in the settled dust of the facilities under study. In conclusion, endotoxin in the concentrations detected in this study may present it respiratory hazard to both livestock animals and farm workers. Thus, the prevention measures aiming to lower the exposure to endotoxin in livestock facilities are highly desirable. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
chromatography-mass spectrometry, gas, settled dust, bacterial endotoxin, animal houses, Limulus test
in
Bulletin of the Veterinary Institute in Puławy
volume
53
issue
1
pages
37 - 41
publisher
National Veterinary Research Institute
external identifiers
  • wos:000264793900007
  • scopus:69249222649
ISSN
0042-4870
language
English
LU publication?
yes
id
21de880e-d9ad-4993-89bc-526fe557e94f (old id 1401169)
date added to LUP
2009-06-15 12:35:12
date last changed
2017-01-01 05:51:18
@article{21de880e-d9ad-4993-89bc-526fe557e94f,
  abstract     = {Samples of settled dust were collected in 14 animal houses and examined for the presence of bacterial endotoxin with the Limulus (LAL) test and the gas chromatography - tandem mass spectrometry (GC-MSMS) technique, based oil the detection of 3-hydroxy fatty acids (3-OH-FAs) as chemical markers of the endotoxin lipopolysaccharide. The median concentrations of the endotoxin in dust determined with LAL test in sheep sheds, poultry houses, and horse stables were 15,687.5 mu g/g, 8,081.8 mu g/g, and 79.3 mu g/g, respectively, while those determined with the GC-MSMS technique were 868.0 mu g/g, 580.0 mu g/g, and 496.0 mu g/g, respectively. Statistical comparison of the results yielded with LAL test and GC-MSMS technique revealed a weak correlation between both methods. Fatty acids with 14-16 carbon chains (3-OH-C-14 and 3-OH-C-16) were predominant in the settled dust of the facilities under study. In conclusion, endotoxin in the concentrations detected in this study may present it respiratory hazard to both livestock animals and farm workers. Thus, the prevention measures aiming to lower the exposure to endotoxin in livestock facilities are highly desirable.},
  author       = {Pomorska, Dorota and Larsson, Lennart and Skorska, Czeslawa and Sitkowska, Jolanta and Dutkiewicz, Jacek},
  issn         = {0042-4870},
  keyword      = {chromatography-mass spectrometry,gas,settled dust,bacterial endotoxin,animal houses,Limulus test},
  language     = {eng},
  number       = {1},
  pages        = {37--41},
  publisher    = {National Veterinary Research Institute},
  series       = {Bulletin of the Veterinary Institute in Puławy},
  title        = {Levels Of Bacterial Endotoxin In The Samples Of Settled Dust Collected In Animal Houses},
  volume       = {53},
  year         = {2009},
}