Behavioral characterization of a unilateral 6-OHDA-lesion model of Parkinson's disease in mice.
(2005) In Behavioural Brain Research 162(1). p.1-10- Abstract
- Parkinson's disease (PD) is one of the most common neurodegenerative disorders. Several toxin-induced animals models simulate the motor deficits occurring in PD. Among them, the unilateral 6-hydroxydopamine (6-OHDA) model is frequently used in rats and has the advantage of presenting side-biased motor impairments. However, the behavioral consequences of a unilateral 6-OHDA-lesion have, so far, not been described in detail in mice. The aim of this study was to characterize mice with unilateral 6-OHDA-lesions placed in the median forebrain bundle using several motor behavioral tests in order to identify the most suitable predictor of nigral cell loss. Mice underwent various drug-induced (amphetamine- and apomorphine-induced rotation) and... (More)
- Parkinson's disease (PD) is one of the most common neurodegenerative disorders. Several toxin-induced animals models simulate the motor deficits occurring in PD. Among them, the unilateral 6-hydroxydopamine (6-OHDA) model is frequently used in rats and has the advantage of presenting side-biased motor impairments. However, the behavioral consequences of a unilateral 6-OHDA-lesion have, so far, not been described in detail in mice. The aim of this study was to characterize mice with unilateral 6-OHDA-lesions placed in the median forebrain bundle using several motor behavioral tests in order to identify the most suitable predictor of nigral cell loss. Mice underwent various drug-induced (amphetamine- and apomorphine-induced rotation) and spontaneous motor tests (cylinder, rotarod, elevated body swing, and stride length test). The amphetamine-induced rotation test, the cylinder and the rotarod test were most sensitive and reliable in detecting loss of tyrosine hydroxylase-immunoreactive cells in the substantia nigra. This study demonstrates that substantial and stable unilateral 6-OHDA-induced lesions can be established in mice, and that these lesions can be functionally assessed using several different side-bias-based behavioral tests. This mouse model offers the opportunity to use transgenic mouse strains and study the interactions between genes of interest and toxins in relation to Parkinson's disease etiology in the future. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/140446
- author
- Iancu, Ruxandra LU ; Mohapel, Paul LU ; Brundin, Patrik LU and Paul-Visse, Gesine LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Parkinson disease, Mouse model, 6-OHDA, Behavioral tests, Tyrosine hydroxylase
- in
- Behavioural Brain Research
- volume
- 162
- issue
- 1
- pages
- 1 - 10
- publisher
- Elsevier
- external identifiers
-
- wos:000229795800001
- pmid:15922062
- scopus:19444370500
- ISSN
- 0166-4328
- DOI
- 10.1016/j.bbr.2005.02.023
- language
- English
- LU publication?
- yes
- id
- 215384ef-6d9f-4665-bb1c-cd2fa5b30e33 (old id 140446)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15922062&query_hl=92
- date added to LUP
- 2016-04-01 12:02:10
- date last changed
- 2022-04-13 05:04:58
@article{215384ef-6d9f-4665-bb1c-cd2fa5b30e33,
abstract = {{Parkinson's disease (PD) is one of the most common neurodegenerative disorders. Several toxin-induced animals models simulate the motor deficits occurring in PD. Among them, the unilateral 6-hydroxydopamine (6-OHDA) model is frequently used in rats and has the advantage of presenting side-biased motor impairments. However, the behavioral consequences of a unilateral 6-OHDA-lesion have, so far, not been described in detail in mice. The aim of this study was to characterize mice with unilateral 6-OHDA-lesions placed in the median forebrain bundle using several motor behavioral tests in order to identify the most suitable predictor of nigral cell loss. Mice underwent various drug-induced (amphetamine- and apomorphine-induced rotation) and spontaneous motor tests (cylinder, rotarod, elevated body swing, and stride length test). The amphetamine-induced rotation test, the cylinder and the rotarod test were most sensitive and reliable in detecting loss of tyrosine hydroxylase-immunoreactive cells in the substantia nigra. This study demonstrates that substantial and stable unilateral 6-OHDA-induced lesions can be established in mice, and that these lesions can be functionally assessed using several different side-bias-based behavioral tests. This mouse model offers the opportunity to use transgenic mouse strains and study the interactions between genes of interest and toxins in relation to Parkinson's disease etiology in the future.}},
author = {{Iancu, Ruxandra and Mohapel, Paul and Brundin, Patrik and Paul-Visse, Gesine}},
issn = {{0166-4328}},
keywords = {{Parkinson disease; Mouse model; 6-OHDA; Behavioral tests; Tyrosine hydroxylase}},
language = {{eng}},
number = {{1}},
pages = {{1--10}},
publisher = {{Elsevier}},
series = {{Behavioural Brain Research}},
title = {{Behavioral characterization of a unilateral 6-OHDA-lesion model of Parkinson's disease in mice.}},
url = {{https://lup.lub.lu.se/search/files/2753133/624768.pdf}},
doi = {{10.1016/j.bbr.2005.02.023}},
volume = {{162}},
year = {{2005}},
}