Advanced

Behavioral characterization of a unilateral 6-OHDA-lesion model of Parkinson's disease in mice.

Iancu, Ruxandra LU ; Mohapel, Paul LU ; Brundin, Patrik LU and Paul-Visse, Gesine LU (2005) In Behavioural Brain Research 162(1). p.1-10
Abstract
Parkinson's disease (PD) is one of the most common neurodegenerative disorders. Several toxin-induced animals models simulate the motor deficits occurring in PD. Among them, the unilateral 6-hydroxydopamine (6-OHDA) model is frequently used in rats and has the advantage of presenting side-biased motor impairments. However, the behavioral consequences of a unilateral 6-OHDA-lesion have, so far, not been described in detail in mice. The aim of this study was to characterize mice with unilateral 6-OHDA-lesions placed in the median forebrain bundle using several motor behavioral tests in order to identify the most suitable predictor of nigral cell loss. Mice underwent various drug-induced (amphetamine- and apomorphine-induced rotation) and... (More)
Parkinson's disease (PD) is one of the most common neurodegenerative disorders. Several toxin-induced animals models simulate the motor deficits occurring in PD. Among them, the unilateral 6-hydroxydopamine (6-OHDA) model is frequently used in rats and has the advantage of presenting side-biased motor impairments. However, the behavioral consequences of a unilateral 6-OHDA-lesion have, so far, not been described in detail in mice. The aim of this study was to characterize mice with unilateral 6-OHDA-lesions placed in the median forebrain bundle using several motor behavioral tests in order to identify the most suitable predictor of nigral cell loss. Mice underwent various drug-induced (amphetamine- and apomorphine-induced rotation) and spontaneous motor tests (cylinder, rotarod, elevated body swing, and stride length test). The amphetamine-induced rotation test, the cylinder and the rotarod test were most sensitive and reliable in detecting loss of tyrosine hydroxylase-immunoreactive cells in the substantia nigra. This study demonstrates that substantial and stable unilateral 6-OHDA-induced lesions can be established in mice, and that these lesions can be functionally assessed using several different side-bias-based behavioral tests. This mouse model offers the opportunity to use transgenic mouse strains and study the interactions between genes of interest and toxins in relation to Parkinson's disease etiology in the future. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Parkinson disease, Mouse model, 6-OHDA, Behavioral tests, Tyrosine hydroxylase
in
Behavioural Brain Research
volume
162
issue
1
pages
1 - 10
publisher
Elsevier
external identifiers
  • wos:000229795800001
  • pmid:15922062
  • scopus:19444370500
ISSN
0166-4328
DOI
10.1016/j.bbr.2005.02.023
language
English
LU publication?
yes
id
215384ef-6d9f-4665-bb1c-cd2fa5b30e33 (old id 140446)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15922062&query_hl=92
date added to LUP
2007-07-13 14:13:52
date last changed
2017-10-08 03:33:38
@article{215384ef-6d9f-4665-bb1c-cd2fa5b30e33,
  abstract     = {Parkinson's disease (PD) is one of the most common neurodegenerative disorders. Several toxin-induced animals models simulate the motor deficits occurring in PD. Among them, the unilateral 6-hydroxydopamine (6-OHDA) model is frequently used in rats and has the advantage of presenting side-biased motor impairments. However, the behavioral consequences of a unilateral 6-OHDA-lesion have, so far, not been described in detail in mice. The aim of this study was to characterize mice with unilateral 6-OHDA-lesions placed in the median forebrain bundle using several motor behavioral tests in order to identify the most suitable predictor of nigral cell loss. Mice underwent various drug-induced (amphetamine- and apomorphine-induced rotation) and spontaneous motor tests (cylinder, rotarod, elevated body swing, and stride length test). The amphetamine-induced rotation test, the cylinder and the rotarod test were most sensitive and reliable in detecting loss of tyrosine hydroxylase-immunoreactive cells in the substantia nigra. This study demonstrates that substantial and stable unilateral 6-OHDA-induced lesions can be established in mice, and that these lesions can be functionally assessed using several different side-bias-based behavioral tests. This mouse model offers the opportunity to use transgenic mouse strains and study the interactions between genes of interest and toxins in relation to Parkinson's disease etiology in the future.},
  author       = {Iancu, Ruxandra and Mohapel, Paul and Brundin, Patrik and Paul-Visse, Gesine},
  issn         = {0166-4328},
  keyword      = {Parkinson disease,Mouse model,6-OHDA,Behavioral tests,Tyrosine hydroxylase},
  language     = {eng},
  number       = {1},
  pages        = {1--10},
  publisher    = {Elsevier},
  series       = {Behavioural Brain Research},
  title        = {Behavioral characterization of a unilateral 6-OHDA-lesion model of Parkinson's disease in mice.},
  url          = {http://dx.doi.org/10.1016/j.bbr.2005.02.023},
  volume       = {162},
  year         = {2005},
}