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Membrane-Less Biofuel Cell Based on Cellobiose Dehydrogenase (Anode)/Laccase (Cathode) Wired via Specific Os-Redox Polymers

Stoica, L.; Dimcheva, N.; Ackermann, Y.; Karnicka, K.; Guschin, D. A.; Kulesza, P. J.; Rogalski, J.; Haltrich, D.; Ludwig, R. and Gorton, Lo LU , et al. (2009) In Fuel Cells 9(1). p.53-62
Abstract
A membrane-free biofuel cell (BFC) is reported based on enzymes wired to graphite electrodes by means of Os-complex modified redox polymers. For the anode cellobiose dehydrogenase (CDH) is used as a biocatalyst whereas for the cathode a laccase was applied. This laccase is a high-potential laccase and hence able to reduce O-2 to H2O at a formal potential higher than +500 mV versus Ag/AgCl. In order to establish efficient electrochemical contact between the enzymes and graphite electrodes electrodeposition polymers containing Os-complex with specifically designed monomer compositions and formal potentials of the coordinatively bound Os-complex were synthesised and used to wire the enzymes to the electrodes. The newly designed CDH/Os-redox... (More)
A membrane-free biofuel cell (BFC) is reported based on enzymes wired to graphite electrodes by means of Os-complex modified redox polymers. For the anode cellobiose dehydrogenase (CDH) is used as a biocatalyst whereas for the cathode a laccase was applied. This laccase is a high-potential laccase and hence able to reduce O-2 to H2O at a formal potential higher than +500 mV versus Ag/AgCl. In order to establish efficient electrochemical contact between the enzymes and graphite electrodes electrodeposition polymers containing Os-complex with specifically designed monomer compositions and formal potentials of the coordinatively bound Os-complex were synthesised and used to wire the enzymes to the electrodes. The newly designed CDH/Os-redox polymer anode was characterised at different pH values and optimised with respect to the nature of the polymer and the enzyme-to-polymer ratio. The resulting BFC was evaluated running on beta-lactose as a fuel and air/O-2 as an oxidising agent. The power output, the maximum current density and the electromotor force (E-emf) were found to be affected by the pH value, resulting in a maximum power output of 1.9 mu W cm(-2) reached at pH 4.3, a maximum current density of about 13 mu A cm(-2) at pH 3.5, and the highest E-emf approaching 600 mV at pH 4.0. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Os-Redox Polymer, Lactose, Laccase, Cellobiose Dehydrogenase, Biofuel Cell, Cellobiose, Oxygen Reduction Reaction
in
Fuel Cells
volume
9
issue
1
pages
53 - 62
publisher
John Wiley & Sons
external identifiers
  • wos:000264061700007
  • scopus:60849133649
ISSN
1615-6854
DOI
10.1002/fuce.200800033
language
English
LU publication?
yes
id
32b440b0-70a1-4549-8065-4ae262513b5b (old id 1404914)
date added to LUP
2009-06-15 09:57:39
date last changed
2017-09-24 03:37:37
@article{32b440b0-70a1-4549-8065-4ae262513b5b,
  abstract     = {A membrane-free biofuel cell (BFC) is reported based on enzymes wired to graphite electrodes by means of Os-complex modified redox polymers. For the anode cellobiose dehydrogenase (CDH) is used as a biocatalyst whereas for the cathode a laccase was applied. This laccase is a high-potential laccase and hence able to reduce O-2 to H2O at a formal potential higher than +500 mV versus Ag/AgCl. In order to establish efficient electrochemical contact between the enzymes and graphite electrodes electrodeposition polymers containing Os-complex with specifically designed monomer compositions and formal potentials of the coordinatively bound Os-complex were synthesised and used to wire the enzymes to the electrodes. The newly designed CDH/Os-redox polymer anode was characterised at different pH values and optimised with respect to the nature of the polymer and the enzyme-to-polymer ratio. The resulting BFC was evaluated running on beta-lactose as a fuel and air/O-2 as an oxidising agent. The power output, the maximum current density and the electromotor force (E-emf) were found to be affected by the pH value, resulting in a maximum power output of 1.9 mu W cm(-2) reached at pH 4.3, a maximum current density of about 13 mu A cm(-2) at pH 3.5, and the highest E-emf approaching 600 mV at pH 4.0.},
  author       = {Stoica, L. and Dimcheva, N. and Ackermann, Y. and Karnicka, K. and Guschin, D. A. and Kulesza, P. J. and Rogalski, J. and Haltrich, D. and Ludwig, R. and Gorton, Lo and Schuhmann, W.},
  issn         = {1615-6854},
  keyword      = {Os-Redox Polymer,Lactose,Laccase,Cellobiose Dehydrogenase,Biofuel Cell,Cellobiose,Oxygen Reduction Reaction},
  language     = {eng},
  number       = {1},
  pages        = {53--62},
  publisher    = {John Wiley & Sons},
  series       = {Fuel Cells},
  title        = {Membrane-Less Biofuel Cell Based on Cellobiose Dehydrogenase (Anode)/Laccase (Cathode) Wired via Specific Os-Redox Polymers},
  url          = {http://dx.doi.org/10.1002/fuce.200800033},
  volume       = {9},
  year         = {2009},
}