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Insulin sensitivity increase after calcium supplementation and change in intraplatelet calcium and sodium-hydrogen exchange in hypertensive patients with Type 2 diabetes

Pikilidou, M. I.; Lasaridis, A. N.; Sarafidis, P. A.; Befani, C. D.; Koliakos, G. G.; Tziolas, I. M.; Kazakos, K. A.; Yovos, J. G. and Nilsson, Peter LU (2009) In Diabetic Medicine 26(3). p.211-219
Abstract
To investigate the effect of oral calcium (Ca2+) supplementation on insulin sensitivity measured by the euglycaemic hyperinsulinaemic clamp, intraplatelet cationic concentration of Ca2+ ([Ca2+](i)) and the transmembrane sodium-hydrogen exchanger (NHE) activity in erythrocytes in subjects with Type 2 diabetes and hypertension. In this parallel randomized controlled single-blinded trial, 31 patients were allocated to receive either 1500 mg of Ca2+ orally, daily (n = 15) or no treatment (n = 16) for 8 weeks. At baseline and at the end of the 8-week period insulin sensitivity, [Ca2+](i) and the first isoform of NHE (NHE-1) activity were measured. At the end of the study, subjects who received Ca2+ supplementation showed higher insulin... (More)
To investigate the effect of oral calcium (Ca2+) supplementation on insulin sensitivity measured by the euglycaemic hyperinsulinaemic clamp, intraplatelet cationic concentration of Ca2+ ([Ca2+](i)) and the transmembrane sodium-hydrogen exchanger (NHE) activity in erythrocytes in subjects with Type 2 diabetes and hypertension. In this parallel randomized controlled single-blinded trial, 31 patients were allocated to receive either 1500 mg of Ca2+ orally, daily (n = 15) or no treatment (n = 16) for 8 weeks. At baseline and at the end of the 8-week period insulin sensitivity, [Ca2+](i) and the first isoform of NHE (NHE-1) activity were measured. At the end of the study, subjects who received Ca2+ supplementation showed higher insulin sensitivity (Delta M-value 0.32 +/- 0.5 mmol/min P < 0.05) and lower [Ca2+](i) (125.0 +/- 24.7 to 80.4 +/- 10.6 nmol/l, P < 0.05, mean +/- sem) and NHE-1 activity (79.5 +/- 10.0 to 52.1 +/- 6.4 mmol Na/l red cell/h, P < 0.05). None of the above parameters were changed in the control group. Simple regression analysis demonstrated the change in [Ca2+](i) significantly determined insulin sensitivity change (beta = -0.36, P < 0.05). Oral Ca2+ supplementation improves insulin sensitivity in patients with Type 2 diabetes and hypertension. These changes are likely to be mediated by changes in intracellular ionic Ca2+. NHE-1 activity was also reduced after Ca2+ supplementation but its role in insulin sensitivity requires further investigation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
calcium supplementation, hyperinsulinaemic clamp, insulin sensitivity
in
Diabetic Medicine
volume
26
issue
3
pages
211 - 219
publisher
Wiley-Blackwell
external identifiers
  • wos:000264163800003
  • scopus:62449232030
ISSN
1464-5491
DOI
10.1111/j.1464-5491.2009.02673.x
language
English
LU publication?
yes
id
b9260dbf-76f9-499d-92af-601389ae2dcf (old id 1404945)
date added to LUP
2009-06-15 09:58:33
date last changed
2017-08-13 04:08:01
@article{b9260dbf-76f9-499d-92af-601389ae2dcf,
  abstract     = {To investigate the effect of oral calcium (Ca2+) supplementation on insulin sensitivity measured by the euglycaemic hyperinsulinaemic clamp, intraplatelet cationic concentration of Ca2+ ([Ca2+](i)) and the transmembrane sodium-hydrogen exchanger (NHE) activity in erythrocytes in subjects with Type 2 diabetes and hypertension. In this parallel randomized controlled single-blinded trial, 31 patients were allocated to receive either 1500 mg of Ca2+ orally, daily (n = 15) or no treatment (n = 16) for 8 weeks. At baseline and at the end of the 8-week period insulin sensitivity, [Ca2+](i) and the first isoform of NHE (NHE-1) activity were measured. At the end of the study, subjects who received Ca2+ supplementation showed higher insulin sensitivity (Delta M-value 0.32 +/- 0.5 mmol/min P &lt; 0.05) and lower [Ca2+](i) (125.0 +/- 24.7 to 80.4 +/- 10.6 nmol/l, P &lt; 0.05, mean +/- sem) and NHE-1 activity (79.5 +/- 10.0 to 52.1 +/- 6.4 mmol Na/l red cell/h, P &lt; 0.05). None of the above parameters were changed in the control group. Simple regression analysis demonstrated the change in [Ca2+](i) significantly determined insulin sensitivity change (beta = -0.36, P &lt; 0.05). Oral Ca2+ supplementation improves insulin sensitivity in patients with Type 2 diabetes and hypertension. These changes are likely to be mediated by changes in intracellular ionic Ca2+. NHE-1 activity was also reduced after Ca2+ supplementation but its role in insulin sensitivity requires further investigation.},
  author       = {Pikilidou, M. I. and Lasaridis, A. N. and Sarafidis, P. A. and Befani, C. D. and Koliakos, G. G. and Tziolas, I. M. and Kazakos, K. A. and Yovos, J. G. and Nilsson, Peter},
  issn         = {1464-5491},
  keyword      = {calcium supplementation,hyperinsulinaemic clamp,insulin sensitivity},
  language     = {eng},
  number       = {3},
  pages        = {211--219},
  publisher    = {Wiley-Blackwell},
  series       = {Diabetic Medicine},
  title        = {Insulin sensitivity increase after calcium supplementation and change in intraplatelet calcium and sodium-hydrogen exchange in hypertensive patients with Type 2 diabetes},
  url          = {http://dx.doi.org/10.1111/j.1464-5491.2009.02673.x},
  volume       = {26},
  year         = {2009},
}