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Characterization of a continuous supermacroporous monolithic matrix for chromatographic separation of large bioparticles

Persson, Patrik LU ; Zaushitsyna, Oksana LU ; Plieva, Fatima LU ; Galaev, Igor LU ; Mattiasson, Bo LU ; Nilsson, Bernt LU and Axelsson, Anders LU (2004) In Biotechnology and Bioengineering 88(2). p.224-236
Abstract
A continuous supermacroporous monolithic chromatographic matrix has been characterized using a capillary model, experimental breakthrough curves, and pressure drop experiments. The model describes the convective flow and its dispersive mixing effects, mass transfer resistance, pore size distribution, and the adsorption behavior of the monolithic matrix. It is possible to determine an effective pore size distribution by fitting the capillary model to experimental breakthrough curves and pressure drop experiments. The model is able to describe the flow rate dependence of the experimental breakthrough curves. Mass transport resistance was due to: (i) dispersive mixing effects in the convective flow in the pores; and (ii) slow diffusion in the... (More)
A continuous supermacroporous monolithic chromatographic matrix has been characterized using a capillary model, experimental breakthrough curves, and pressure drop experiments. The model describes the convective flow and its dispersive mixing effects, mass transfer resistance, pore size distribution, and the adsorption behavior of the monolithic matrix. It is possible to determine an effective pore size distribution by fitting the capillary model to experimental breakthrough curves and pressure drop experiments. The model is able to describe the flow rate dependence of the experimental breakthrough curves. Mass transport resistance was due to: (i) dispersive mixing effects in the convective flow in the pores; and (ii) slow diffusion in the stagnant film covering the surface within each pore, under adsorption conditions. The monolithic matrix can be described by a very narrow pore size distribution, illustrating one of the advantages of the gel. A broader pore size distribution results in increased band broadening. This can be studied easily using the model developed in this investigation. (C) 2004 Wiley Periodicals, Inc. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biotechnology and Bioengineering
volume
88
issue
2
pages
224 - 236
publisher
John Wiley & Sons
external identifiers
  • wos:000224266600009
  • pmid:15449292
  • scopus:7044250699
ISSN
1097-0290
DOI
10.1002/bit.20236
language
English
LU publication?
yes
id
38dff016-54ef-418a-90a3-981cf479ab69 (old id 140560)
date added to LUP
2007-06-25 15:27:35
date last changed
2017-07-09 03:33:59
@article{38dff016-54ef-418a-90a3-981cf479ab69,
  abstract     = {A continuous supermacroporous monolithic chromatographic matrix has been characterized using a capillary model, experimental breakthrough curves, and pressure drop experiments. The model describes the convective flow and its dispersive mixing effects, mass transfer resistance, pore size distribution, and the adsorption behavior of the monolithic matrix. It is possible to determine an effective pore size distribution by fitting the capillary model to experimental breakthrough curves and pressure drop experiments. The model is able to describe the flow rate dependence of the experimental breakthrough curves. Mass transport resistance was due to: (i) dispersive mixing effects in the convective flow in the pores; and (ii) slow diffusion in the stagnant film covering the surface within each pore, under adsorption conditions. The monolithic matrix can be described by a very narrow pore size distribution, illustrating one of the advantages of the gel. A broader pore size distribution results in increased band broadening. This can be studied easily using the model developed in this investigation. (C) 2004 Wiley Periodicals, Inc.},
  author       = {Persson, Patrik and Zaushitsyna, Oksana and Plieva, Fatima and Galaev, Igor and Mattiasson, Bo and Nilsson, Bernt and Axelsson, Anders},
  issn         = {1097-0290},
  language     = {eng},
  number       = {2},
  pages        = {224--236},
  publisher    = {John Wiley & Sons},
  series       = {Biotechnology and Bioengineering},
  title        = {Characterization of a continuous supermacroporous monolithic matrix for chromatographic separation of large bioparticles},
  url          = {http://dx.doi.org/10.1002/bit.20236},
  volume       = {88},
  year         = {2004},
}