Characterization of a continuous supermacroporous monolithic matrix for chromatographic separation of large bioparticles
(2004) In Biotechnology and Bioengineering 88(2). p.224-236- Abstract
- A continuous supermacroporous monolithic chromatographic matrix has been characterized using a capillary model, experimental breakthrough curves, and pressure drop experiments. The model describes the convective flow and its dispersive mixing effects, mass transfer resistance, pore size distribution, and the adsorption behavior of the monolithic matrix. It is possible to determine an effective pore size distribution by fitting the capillary model to experimental breakthrough curves and pressure drop experiments. The model is able to describe the flow rate dependence of the experimental breakthrough curves. Mass transport resistance was due to: (i) dispersive mixing effects in the convective flow in the pores; and (ii) slow diffusion in the... (More)
- A continuous supermacroporous monolithic chromatographic matrix has been characterized using a capillary model, experimental breakthrough curves, and pressure drop experiments. The model describes the convective flow and its dispersive mixing effects, mass transfer resistance, pore size distribution, and the adsorption behavior of the monolithic matrix. It is possible to determine an effective pore size distribution by fitting the capillary model to experimental breakthrough curves and pressure drop experiments. The model is able to describe the flow rate dependence of the experimental breakthrough curves. Mass transport resistance was due to: (i) dispersive mixing effects in the convective flow in the pores; and (ii) slow diffusion in the stagnant film covering the surface within each pore, under adsorption conditions. The monolithic matrix can be described by a very narrow pore size distribution, illustrating one of the advantages of the gel. A broader pore size distribution results in increased band broadening. This can be studied easily using the model developed in this investigation. (C) 2004 Wiley Periodicals, Inc. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/140560
- author
- Persson, Patrik LU ; Zaushitsyna, Oksana LU ; Plieva, Fatima LU ; Galaev, Igor LU ; Mattiasson, Bo LU ; Nilsson, Bernt LU and Axelsson, Anders LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biotechnology and Bioengineering
- volume
- 88
- issue
- 2
- pages
- 224 - 236
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000224266600009
- pmid:15449292
- scopus:7044250699
- pmid:15449292
- ISSN
- 1097-0290
- DOI
- 10.1002/bit.20236
- language
- English
- LU publication?
- yes
- id
- 38dff016-54ef-418a-90a3-981cf479ab69 (old id 140560)
- date added to LUP
- 2016-04-01 11:49:19
- date last changed
- 2023-09-15 09:06:58
@article{38dff016-54ef-418a-90a3-981cf479ab69,
abstract = {{A continuous supermacroporous monolithic chromatographic matrix has been characterized using a capillary model, experimental breakthrough curves, and pressure drop experiments. The model describes the convective flow and its dispersive mixing effects, mass transfer resistance, pore size distribution, and the adsorption behavior of the monolithic matrix. It is possible to determine an effective pore size distribution by fitting the capillary model to experimental breakthrough curves and pressure drop experiments. The model is able to describe the flow rate dependence of the experimental breakthrough curves. Mass transport resistance was due to: (i) dispersive mixing effects in the convective flow in the pores; and (ii) slow diffusion in the stagnant film covering the surface within each pore, under adsorption conditions. The monolithic matrix can be described by a very narrow pore size distribution, illustrating one of the advantages of the gel. A broader pore size distribution results in increased band broadening. This can be studied easily using the model developed in this investigation. (C) 2004 Wiley Periodicals, Inc.}},
author = {{Persson, Patrik and Zaushitsyna, Oksana and Plieva, Fatima and Galaev, Igor and Mattiasson, Bo and Nilsson, Bernt and Axelsson, Anders}},
issn = {{1097-0290}},
language = {{eng}},
number = {{2}},
pages = {{224--236}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Biotechnology and Bioengineering}},
title = {{Characterization of a continuous supermacroporous monolithic matrix for chromatographic separation of large bioparticles}},
url = {{http://dx.doi.org/10.1002/bit.20236}},
doi = {{10.1002/bit.20236}},
volume = {{88}},
year = {{2004}},
}