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Characterization of polyacrylamide based monolithic columns

Plieva, Fatima LU ; Andersson, Jonatan LU ; Galaev, Igor LU and Mattiasson, Bo LU (2004) In Journal of Separation Science 27(10-11). p.828-836
Abstract
Supermacroporous monolithic polyacrylamide (pAAm)-based columns have been prepared by radical cryo-copolymerization (copolymerization in the moderately frozen system) of acrylamide with functional co-monomer, allyl glycidyl ether (AGE), and cross-linker NN'-methylene-bis-acrylamide (MBAAm) directly in glass columns (ID 10 mm). The monolithic columns have uniform supermacroporous sponge-like structure with interconnected supermacropores of pore size 5-100 mum. The monoliths can be dried and stored in the dry state. High mechanical stability of the monoliths allowed sterilization by autoclaving. Column-to-column reproducibility of pAAm-monoliths was demonstrated on 5 monolithic columns from different batches prepared under the same... (More)
Supermacroporous monolithic polyacrylamide (pAAm)-based columns have been prepared by radical cryo-copolymerization (copolymerization in the moderately frozen system) of acrylamide with functional co-monomer, allyl glycidyl ether (AGE), and cross-linker NN'-methylene-bis-acrylamide (MBAAm) directly in glass columns (ID 10 mm). The monolithic columns have uniform supermacroporous sponge-like structure with interconnected supermacropores of pore size 5-100 mum. The monoliths can be dried and stored in the dry state. High mechanical stability of the monoliths allowed sterilization by autoclaving. Column-to-column reproducibility of pAAm-monoliths was demonstrated on 5 monolithic columns from different batches prepared under the same cryostructuration conditions. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Separation Science
volume
27
issue
10-11
pages
828 - 836
publisher
John Wiley & Sons
external identifiers
  • pmid:15354560
  • wos:000222856300009
  • scopus:4344717800
ISSN
1615-9314
DOI
10.1002/jssc.200401836
language
English
LU publication?
yes
id
c564d004-c25e-4521-bcdf-f8fba0c88640 (old id 140621)
alternative location
http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=PubMed&list_uids=15354560&dopt=Abstract
date added to LUP
2007-07-02 16:34:19
date last changed
2017-10-22 03:42:40
@article{c564d004-c25e-4521-bcdf-f8fba0c88640,
  abstract     = {Supermacroporous monolithic polyacrylamide (pAAm)-based columns have been prepared by radical cryo-copolymerization (copolymerization in the moderately frozen system) of acrylamide with functional co-monomer, allyl glycidyl ether (AGE), and cross-linker NN'-methylene-bis-acrylamide (MBAAm) directly in glass columns (ID 10 mm). The monolithic columns have uniform supermacroporous sponge-like structure with interconnected supermacropores of pore size 5-100 mum. The monoliths can be dried and stored in the dry state. High mechanical stability of the monoliths allowed sterilization by autoclaving. Column-to-column reproducibility of pAAm-monoliths was demonstrated on 5 monolithic columns from different batches prepared under the same cryostructuration conditions.},
  author       = {Plieva, Fatima and Andersson, Jonatan and Galaev, Igor and Mattiasson, Bo},
  issn         = {1615-9314},
  language     = {eng},
  number       = {10-11},
  pages        = {828--836},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Separation Science},
  title        = {Characterization of polyacrylamide based monolithic columns},
  url          = {http://dx.doi.org/10.1002/jssc.200401836},
  volume       = {27},
  year         = {2004},
}