Microarrays based on affinity-tagged SCFV antibodies: Sensitive detection of analyte in complex proteomes
(2005) 8th International Conference on Miniaturized Systems for Chemistry and Life Sciences p.264-266- Abstract
- Antibody microarray is a novel technology that will allow us to efficiently perform global proteome analysis. Performing rapid and multiplexed analysis on complex proteomes, such as human serum, targeting also low-abundant analytes will, however, place high demands upon the microarray design with respect to logistics, specificity and sensitivity. In this study, we have shown that non-purified affinity-tagged scFv antibody fragments could be directly applied as probes thereby eliminating the need for any time-consuming pre-purification steps. Further, highly functional arrays were obtained providing an assay sensitivity in the pM to fM range. In fact, 300 zeptomole analyte (approx. 5,000 molecules) may be sufficient for detection. Moreover,... (More)
- Antibody microarray is a novel technology that will allow us to efficiently perform global proteome analysis. Performing rapid and multiplexed analysis on complex proteomes, such as human serum, targeting also low-abundant analytes will, however, place high demands upon the microarray design with respect to logistics, specificity and sensitivity. In this study, we have shown that non-purified affinity-tagged scFv antibody fragments could be directly applied as probes thereby eliminating the need for any time-consuming pre-purification steps. Further, highly functional arrays were obtained providing an assay sensitivity in the pM to fM range. In fact, 300 zeptomole analyte (approx. 5,000 molecules) may be sufficient for detection. Moreover, highly complex proteomes could be analysed without impairing the specificity and sensitivity of the setups. This is a pre-requisite for the design of high-density antibody arrays applied in high-throughput proteomics. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1406240
- author
- Wingren, Christer LU ; Steinhauer, Cornelia LU ; Ingvarsson, Johan LU ; Persson, E ; Larsson, Kristina LU and Borrebaeck, Carl LU
- organization
- publishing date
- 2005
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- scFv, antibody microarrays, antibody chips, sensitivity, affinity-tag
- host publication
- Micro Total Analysis Systems 2004, Vol 2
- issue
- 297
- pages
- 264 - 266
- publisher
- Royal Society of Chemistry
- conference name
- 8th International Conference on Miniaturized Systems for Chemistry and Life Sciences
- conference location
- Malmo, Sweden
- conference dates
- 2004-09-26 - 2004-09-30
- external identifiers
-
- wos:000229017400087
- ISSN
- 0260-6291
- ISBN
- 9780854048960
- language
- English
- LU publication?
- yes
- id
- 12644c17-0049-4f09-aee3-0e59f785c98a (old id 1406240)
- date added to LUP
- 2016-04-01 16:31:16
- date last changed
- 2018-11-21 20:42:04
@inproceedings{12644c17-0049-4f09-aee3-0e59f785c98a, abstract = {{Antibody microarray is a novel technology that will allow us to efficiently perform global proteome analysis. Performing rapid and multiplexed analysis on complex proteomes, such as human serum, targeting also low-abundant analytes will, however, place high demands upon the microarray design with respect to logistics, specificity and sensitivity. In this study, we have shown that non-purified affinity-tagged scFv antibody fragments could be directly applied as probes thereby eliminating the need for any time-consuming pre-purification steps. Further, highly functional arrays were obtained providing an assay sensitivity in the pM to fM range. In fact, 300 zeptomole analyte (approx. 5,000 molecules) may be sufficient for detection. Moreover, highly complex proteomes could be analysed without impairing the specificity and sensitivity of the setups. This is a pre-requisite for the design of high-density antibody arrays applied in high-throughput proteomics.}}, author = {{Wingren, Christer and Steinhauer, Cornelia and Ingvarsson, Johan and Persson, E and Larsson, Kristina and Borrebaeck, Carl}}, booktitle = {{Micro Total Analysis Systems 2004, Vol 2}}, isbn = {{9780854048960}}, issn = {{0260-6291}}, keywords = {{scFv; antibody microarrays; antibody chips; sensitivity; affinity-tag}}, language = {{eng}}, number = {{297}}, pages = {{264--266}}, publisher = {{Royal Society of Chemistry}}, title = {{Microarrays based on affinity-tagged SCFV antibodies: Sensitive detection of analyte in complex proteomes}}, year = {{2005}}, }