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Microarrays based on affinity-tagged SCFV antibodies: Sensitive detection of analyte in complex proteomes

Wingren, Christer LU ; Steinhauer, Cornelia LU ; Ingvarsson, Johan LU ; Persson, E; Larsson, Kristina LU and Borrebaeck, Carl LU (2005) 8th International Conference on Miniaturized Systems for Chemistry and Life Sciences In Micro Total Analysis Systems 2004, Vol 2 p.264-266
Abstract
Antibody microarray is a novel technology that will allow us to efficiently perform global proteome analysis. Performing rapid and multiplexed analysis on complex proteomes, such as human serum, targeting also low-abundant analytes will, however, place high demands upon the microarray design with respect to logistics, specificity and sensitivity. In this study, we have shown that non-purified affinity-tagged scFv antibody fragments could be directly applied as probes thereby eliminating the need for any time-consuming pre-purification steps. Further, highly functional arrays were obtained providing an assay sensitivity in the pM to fM range. In fact, 300 zeptomole analyte (approx. 5,000 molecules) may be sufficient for detection. Moreover,... (More)
Antibody microarray is a novel technology that will allow us to efficiently perform global proteome analysis. Performing rapid and multiplexed analysis on complex proteomes, such as human serum, targeting also low-abundant analytes will, however, place high demands upon the microarray design with respect to logistics, specificity and sensitivity. In this study, we have shown that non-purified affinity-tagged scFv antibody fragments could be directly applied as probes thereby eliminating the need for any time-consuming pre-purification steps. Further, highly functional arrays were obtained providing an assay sensitivity in the pM to fM range. In fact, 300 zeptomole analyte (approx. 5,000 molecules) may be sufficient for detection. Moreover, highly complex proteomes could be analysed without impairing the specificity and sensitivity of the setups. This is a pre-requisite for the design of high-density antibody arrays applied in high-throughput proteomics. (Less)
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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
scFv, antibody microarrays, antibody chips, sensitivity, affinity-tag
in
Micro Total Analysis Systems 2004, Vol 2
issue
297
pages
264 - 266
publisher
Royal Society of Chemistry
conference name
8th International Conference on Miniaturized Systems for Chemistry and Life Sciences
external identifiers
  • wos:000229017400087
ISSN
0260-6291
ISBN
9780854048960
language
English
LU publication?
yes
id
12644c17-0049-4f09-aee3-0e59f785c98a (old id 1406240)
date added to LUP
2009-06-08 09:54:16
date last changed
2017-10-10 14:33:51
@inproceedings{12644c17-0049-4f09-aee3-0e59f785c98a,
  abstract     = {Antibody microarray is a novel technology that will allow us to efficiently perform global proteome analysis. Performing rapid and multiplexed analysis on complex proteomes, such as human serum, targeting also low-abundant analytes will, however, place high demands upon the microarray design with respect to logistics, specificity and sensitivity. In this study, we have shown that non-purified affinity-tagged scFv antibody fragments could be directly applied as probes thereby eliminating the need for any time-consuming pre-purification steps. Further, highly functional arrays were obtained providing an assay sensitivity in the pM to fM range. In fact, 300 zeptomole analyte (approx. 5,000 molecules) may be sufficient for detection. Moreover, highly complex proteomes could be analysed without impairing the specificity and sensitivity of the setups. This is a pre-requisite for the design of high-density antibody arrays applied in high-throughput proteomics.},
  author       = {Wingren, Christer and Steinhauer, Cornelia and Ingvarsson, Johan and Persson, E and Larsson, Kristina and Borrebaeck, Carl},
  booktitle    = {Micro Total Analysis Systems 2004, Vol 2},
  isbn         = {9780854048960},
  issn         = {0260-6291},
  keyword      = {scFv,antibody microarrays,antibody chips,sensitivity,affinity-tag},
  language     = {eng},
  number       = {297},
  pages        = {264--266},
  publisher    = {Royal Society of Chemistry},
  title        = {Microarrays based on affinity-tagged SCFV antibodies: Sensitive detection of analyte in complex proteomes},
  year         = {2005},
}