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Characterisation of the microbial community in indoor environments: Critical evaluation of sample storage conditions

Sebastian, Aleksandra LU and Larsson, L (2005) 10th International Conference on Indoor Air Quality and Climate (Indoor Air 2005) In Indoor Air 2005: Proceedings of the 10th International Conference on Indoor Air Quality and Climate, Vols 1-5 p.2421-2424
Abstract
Assessment of indoor air quality in terms of microbial flora is important since inhalation of air-borne microorganisms has been associated e.g. with asthma and allergy. We focus on certain unique microbial markers: muramic acid, marker of bacterial peptidoglycan/biomass; 3-hydroxy fatty acids with 10-18 carbon chains, markers of lipopolysaccharide (LPS, endotoxin) of Gram-negative bacteria; and ergosterol, marker of fungal biomass. An integrated method was developed recently for the determination of these markers in house dust. In brief, samples are subjected to acid/alkaline hydrolysis, purified with extractions, and derivatized for rendering them suitable for analysis with gas chromatography/ion trap mass spectrometry The method can be... (More)
Assessment of indoor air quality in terms of microbial flora is important since inhalation of air-borne microorganisms has been associated e.g. with asthma and allergy. We focus on certain unique microbial markers: muramic acid, marker of bacterial peptidoglycan/biomass; 3-hydroxy fatty acids with 10-18 carbon chains, markers of lipopolysaccharide (LPS, endotoxin) of Gram-negative bacteria; and ergosterol, marker of fungal biomass. An integrated method was developed recently for the determination of these markers in house dust. In brief, samples are subjected to acid/alkaline hydrolysis, purified with extractions, and derivatized for rendering them suitable for analysis with gas chromatography/ion trap mass spectrometry The method can be used for characterisation of microbial communities in indoor environments as regards fungal biomass and endotoxin as well as relative amounts of Gram-negative/Gram-positive bacteria. The present investigation aims to show the influence of different storage conditions on stability of the markers. (Less)
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author
organization
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type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
gas chromatography-mass spectrometry, microbial markers, house dust
in
Indoor Air 2005: Proceedings of the 10th International Conference on Indoor Air Quality and Climate, Vols 1-5
pages
2421 - 2424
publisher
Tsinghua University Press
conference name
10th International Conference on Indoor Air Quality and Climate (Indoor Air 2005)
external identifiers
  • WOS:000233831203089
language
English
LU publication?
yes
id
dbb26f33-1475-408f-b6b2-47e0062f907b (old id 1406424)
date added to LUP
2009-06-04 17:01:44
date last changed
2016-04-16 08:21:34
@inproceedings{dbb26f33-1475-408f-b6b2-47e0062f907b,
  abstract     = {Assessment of indoor air quality in terms of microbial flora is important since inhalation of air-borne microorganisms has been associated e.g. with asthma and allergy. We focus on certain unique microbial markers: muramic acid, marker of bacterial peptidoglycan/biomass; 3-hydroxy fatty acids with 10-18 carbon chains, markers of lipopolysaccharide (LPS, endotoxin) of Gram-negative bacteria; and ergosterol, marker of fungal biomass. An integrated method was developed recently for the determination of these markers in house dust. In brief, samples are subjected to acid/alkaline hydrolysis, purified with extractions, and derivatized for rendering them suitable for analysis with gas chromatography/ion trap mass spectrometry The method can be used for characterisation of microbial communities in indoor environments as regards fungal biomass and endotoxin as well as relative amounts of Gram-negative/Gram-positive bacteria. The present investigation aims to show the influence of different storage conditions on stability of the markers.},
  author       = {Sebastian, Aleksandra and Larsson, L},
  booktitle    = {Indoor Air 2005: Proceedings of the 10th International Conference on Indoor Air Quality and Climate, Vols 1-5},
  keyword      = {gas chromatography-mass spectrometry,microbial markers,house dust},
  language     = {eng},
  pages        = {2421--2424},
  publisher    = {Tsinghua University Press},
  title        = {Characterisation of the microbial community in indoor environments: Critical evaluation of sample storage conditions},
  year         = {2005},
}