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Characterization of supermacroporous monolithic polyacrylamide based matrices designed for chromatography of bioparticles

Plieva, Fatima LU ; Savina, Irina LU ; Deraz, Sahar LU ; Andersson, Jonatan LU ; Galaev, Igor LU and Mattiasson, Bo LU (2004) In Journal of Chromatography. B 807(1). p.129-137
Abstract
Supermacroporous monolithic acrylamide (AAm)-based cryogels were prepared by radical cryo-polymerizaton (polymerization in the moderately frozen system) of AAm with functional monomers and cross-linker N,N'-methylene-bis-acrylamide (MBAAm). Electron microscopy studies revealed supermacroporous structure of the developed cryogels with pore size of 5-100 mum. Cryogel porosity depended on cryo-polymerization conditions. More than 90% of the monolithic bed volume is the interconnected supermacropores filled with water and less than 10% of the monolithic volume is pore walls. The total protein binding capacity (lysozyme in the case of immobilized metal affinity chromatography (IMAC) column and bovine serum albumin (BSA) in the case of... (More)
Supermacroporous monolithic acrylamide (AAm)-based cryogels were prepared by radical cryo-polymerizaton (polymerization in the moderately frozen system) of AAm with functional monomers and cross-linker N,N'-methylene-bis-acrylamide (MBAAm). Electron microscopy studies revealed supermacroporous structure of the developed cryogels with pore size of 5-100 mum. Cryogel porosity depended on cryo-polymerization conditions. More than 90% of the monolithic bed volume is the interconnected supermacropores filled with water and less than 10% of the monolithic volume is pore walls. The total protein binding capacity (lysozyme in the case of immobilized metal affinity chromatography (IMAC) column and bovine serum albumin (BSA) in the case of anion-exchange (AE) column) was independent of the flow rates till 600 cm/h. Chromatographic behavior of E. coli cells when a cell suspension was applied to ion-exchange cryogel columns depended on both the density of functional ligand and the porosity of the cryogel. (C) 2004 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Monolithic column, Bioparticles, Cryogel, Supermacroporous polyacrylamide
in
Journal of Chromatography. B
volume
807
issue
1
pages
129 - 137
publisher
Elsevier
external identifiers
  • wos:000222047600019
  • pmid:15177170
  • scopus:2642511369
ISSN
1873-376X
DOI
10.1016/j.jchromb.2004.01.050
language
English
LU publication?
yes
id
dfb40dbf-46ae-4ada-9c3d-8da746b0c760 (old id 140665)
date added to LUP
2007-07-02 16:21:30
date last changed
2017-11-05 03:39:14
@article{dfb40dbf-46ae-4ada-9c3d-8da746b0c760,
  abstract     = {Supermacroporous monolithic acrylamide (AAm)-based cryogels were prepared by radical cryo-polymerizaton (polymerization in the moderately frozen system) of AAm with functional monomers and cross-linker N,N'-methylene-bis-acrylamide (MBAAm). Electron microscopy studies revealed supermacroporous structure of the developed cryogels with pore size of 5-100 mum. Cryogel porosity depended on cryo-polymerization conditions. More than 90% of the monolithic bed volume is the interconnected supermacropores filled with water and less than 10% of the monolithic volume is pore walls. The total protein binding capacity (lysozyme in the case of immobilized metal affinity chromatography (IMAC) column and bovine serum albumin (BSA) in the case of anion-exchange (AE) column) was independent of the flow rates till 600 cm/h. Chromatographic behavior of E. coli cells when a cell suspension was applied to ion-exchange cryogel columns depended on both the density of functional ligand and the porosity of the cryogel. (C) 2004 Elsevier B.V. All rights reserved.},
  author       = {Plieva, Fatima and Savina, Irina and Deraz, Sahar and Andersson, Jonatan and Galaev, Igor and Mattiasson, Bo},
  issn         = {1873-376X},
  keyword      = {Monolithic column,Bioparticles,Cryogel,Supermacroporous polyacrylamide},
  language     = {eng},
  number       = {1},
  pages        = {129--137},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography. B},
  title        = {Characterization of supermacroporous monolithic polyacrylamide based matrices designed for chromatography of bioparticles},
  url          = {http://dx.doi.org/10.1016/j.jchromb.2004.01.050},
  volume       = {807},
  year         = {2004},
}