Advanced

Serial digital autoradiography with a silicon strip detector as a high resolution imaging modality for TRT Dosimetry

Örbom, Anders LU ; Dahlbom, Magnus; Olafsen, Tove; Wu, Anna M. and Strand, Sven-Erik LU (2007) IEEE Nuclear Science Symposium/Medical Imaging Conference In 2007 IEEE Nuclear Science Symposium Conference Record, vols 1-11 p.4054-4056
Abstract
This study aims to investigate the possibility of implementing serial autoradiography using a silicon strip detector as an imaging modality in pre-clinical radionuclide therapy research, in order to study the effect of non-uniform uptake on absorbed dose distribution and biological response. Tumor tissues expressing CD20 (B-cell lymphoma) or carcinoembryonic antigen (CEA; colorectal cancer) were excised from animals injected with I-131-labelled anti-CD20 or anti-CEA antibodies and antibody fragments. The tumors were cryosectioned at 100 mu m and imaged using a real-time silicon- strip imager with a pixel-size of 50 mu m. Software was developed to correct for image artifacts and to realign the image sections into a volume by a two-step... (More)
This study aims to investigate the possibility of implementing serial autoradiography using a silicon strip detector as an imaging modality in pre-clinical radionuclide therapy research, in order to study the effect of non-uniform uptake on absorbed dose distribution and biological response. Tumor tissues expressing CD20 (B-cell lymphoma) or carcinoembryonic antigen (CEA; colorectal cancer) were excised from animals injected with I-131-labelled anti-CD20 or anti-CEA antibodies and antibody fragments. The tumors were cryosectioned at 100 mu m and imaged using a real-time silicon- strip imager with a pixel-size of 50 mu m. Software was developed to correct for image artifacts and to realign the image sections into a volume by a two-step process with least square error and mutual information registration measures. The realigned volumes were convolved with beta dose point kernels to provide the dose rate distribution for I-131 and Y-90 at the time of sacrifice. Using these volumes, comparisons can be made between uptake and penetration of different antibodies and the dose rate uniformity of different radionuclides. Simulations performed using measured I-131 and I-125 energy spectra showed that energy separation with less than 5% error could be performed with 100 counts per pixel. Imaging and subsequent separation of a sample containing both I-131 and I-125 proved the possibility of simultaneous imaging of two targeting agents in the same tissue. Thinner tissue sections were also set aside and successfully used for H&E staining and immunohistochemistry to enable future comparison of uptake and dose rate in different cell-type populations in the tissue. This method successfully provides high-resolution activity and dose rate volumes and has potential for multi-labeling imaging and co-registration with histology. As a complimentary imaging modality it can aid in investigating the effect of non-uniform uptake. Optimization is still needed in both the sectioning protocol and realignment software. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
in
2007 IEEE Nuclear Science Symposium Conference Record, vols 1-11
pages
4054 - 4056
publisher
IEEE--Institute of Electrical and Electronics Engineers Inc.
conference name
IEEE Nuclear Science Symposium/Medical Imaging Conference
external identifiers
  • wos:000257380403044
  • scopus:48149084791
ISSN
1082-3654
ISBN
978-1-4244-0922-8
DOI
10.1109/NSSMIC.2007.4437018
language
English
LU publication?
yes
id
3c858cc2-f72b-45c9-bf6a-5a752d17af64 (old id 1406779)
date added to LUP
2009-06-04 10:57:08
date last changed
2017-01-01 07:03:50
@inproceedings{3c858cc2-f72b-45c9-bf6a-5a752d17af64,
  abstract     = {This study aims to investigate the possibility of implementing serial autoradiography using a silicon strip detector as an imaging modality in pre-clinical radionuclide therapy research, in order to study the effect of non-uniform uptake on absorbed dose distribution and biological response. Tumor tissues expressing CD20 (B-cell lymphoma) or carcinoembryonic antigen (CEA; colorectal cancer) were excised from animals injected with I-131-labelled anti-CD20 or anti-CEA antibodies and antibody fragments. The tumors were cryosectioned at 100 mu m and imaged using a real-time silicon- strip imager with a pixel-size of 50 mu m. Software was developed to correct for image artifacts and to realign the image sections into a volume by a two-step process with least square error and mutual information registration measures. The realigned volumes were convolved with beta dose point kernels to provide the dose rate distribution for I-131 and Y-90 at the time of sacrifice. Using these volumes, comparisons can be made between uptake and penetration of different antibodies and the dose rate uniformity of different radionuclides. Simulations performed using measured I-131 and I-125 energy spectra showed that energy separation with less than 5% error could be performed with 100 counts per pixel. Imaging and subsequent separation of a sample containing both I-131 and I-125 proved the possibility of simultaneous imaging of two targeting agents in the same tissue. Thinner tissue sections were also set aside and successfully used for H&E staining and immunohistochemistry to enable future comparison of uptake and dose rate in different cell-type populations in the tissue. This method successfully provides high-resolution activity and dose rate volumes and has potential for multi-labeling imaging and co-registration with histology. As a complimentary imaging modality it can aid in investigating the effect of non-uniform uptake. Optimization is still needed in both the sectioning protocol and realignment software.},
  author       = {Örbom, Anders and Dahlbom, Magnus and Olafsen, Tove and Wu, Anna M. and Strand, Sven-Erik},
  booktitle    = {2007 IEEE Nuclear Science Symposium Conference Record, vols 1-11},
  isbn         = {978-1-4244-0922-8},
  issn         = {1082-3654},
  language     = {eng},
  pages        = {4054--4056},
  publisher    = {IEEE--Institute of Electrical and Electronics Engineers Inc.},
  title        = {Serial digital autoradiography with a silicon strip detector as a high resolution imaging modality for TRT Dosimetry},
  url          = {http://dx.doi.org/10.1109/NSSMIC.2007.4437018},
  year         = {2007},
}