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Synthesis of alkylgalactosides using whole cells of Bacillus pseudofirmus species as catalysts

Bradoo, Sapna LU ; Svensson, Ingemar LU ; Santos, Jorge; Plieva, Fatima LU ; Mattiasson, Bo LU and Hatti-Kaul, Rajni LU (2004) In Journal of Biotechnology 110(3). p.273-285
Abstract
Whole cells of alkaliphilic Bacillus pseudofirmus AR-199, induced for beta-galactosidase activity, were used for the synthesis of 1-hexyl-beta-D-galactoside and 1-octyl-beta-D-galactoside, respectively, by transglycosylation reaction between lactose and the corresponding alcohol acceptor. The product yield was strongly influenced by the initial water content in the reaction mixture. Water content of 10% (v/v) was optimal providing 3.6-36 mM hexyl galactoside from 10 to 150 mM lactose, and no secondary product hydrolysis. Product yield could be enhanced by supplementing the reaction mixture with more cells or partly replacing the product with fresh substrate, but was decreased with time to the initial equilibrium level. Cell... (More)
Whole cells of alkaliphilic Bacillus pseudofirmus AR-199, induced for beta-galactosidase activity, were used for the synthesis of 1-hexyl-beta-D-galactoside and 1-octyl-beta-D-galactoside, respectively, by transglycosylation reaction between lactose and the corresponding alcohol acceptor. The product yield was strongly influenced by the initial water content in the reaction mixture. Water content of 10% (v/v) was optimal providing 3.6-36 mM hexyl galactoside from 10 to 150 mM lactose, and no secondary product hydrolysis. Product yield could be enhanced by supplementing the reaction mixture with more cells or partly replacing the product with fresh substrate, but was decreased with time to the initial equilibrium level. Cell permeabilisation or disruption resulted in increased reaction rate and higher product yield but was followed by product hydrolysis. Octyl galactoside synthesis using whole cells was optimal at water content of 2% (v/v) with a yield of 26%. The cells were immobilised in cryogels of polyvinyl alcohol for use in continuous process, where hexyl galactoside was produced with a constant yield of 50% from 50 mM lactose for at least a week. (C) 2004 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
β-Galactosidase, Hexyl-β-galactoside, Octyl-β-galactoside, Transglycosylation, Immobilisation, Cryogel
in
Journal of Biotechnology
volume
110
issue
3
pages
273 - 285
publisher
Elsevier
external identifiers
  • pmid:15163518
  • wos:000221977800007
  • scopus:2442442975
ISSN
1873-4863
DOI
10.1016/j.jbiotec.2004.03.004
language
English
LU publication?
yes
id
5f8e787a-447c-45ca-a78a-505572d296c1 (old id 140678)
date added to LUP
2007-07-02 09:44:23
date last changed
2017-07-02 03:28:30
@article{5f8e787a-447c-45ca-a78a-505572d296c1,
  abstract     = {Whole cells of alkaliphilic Bacillus pseudofirmus AR-199, induced for beta-galactosidase activity, were used for the synthesis of 1-hexyl-beta-D-galactoside and 1-octyl-beta-D-galactoside, respectively, by transglycosylation reaction between lactose and the corresponding alcohol acceptor. The product yield was strongly influenced by the initial water content in the reaction mixture. Water content of 10% (v/v) was optimal providing 3.6-36 mM hexyl galactoside from 10 to 150 mM lactose, and no secondary product hydrolysis. Product yield could be enhanced by supplementing the reaction mixture with more cells or partly replacing the product with fresh substrate, but was decreased with time to the initial equilibrium level. Cell permeabilisation or disruption resulted in increased reaction rate and higher product yield but was followed by product hydrolysis. Octyl galactoside synthesis using whole cells was optimal at water content of 2% (v/v) with a yield of 26%. The cells were immobilised in cryogels of polyvinyl alcohol for use in continuous process, where hexyl galactoside was produced with a constant yield of 50% from 50 mM lactose for at least a week. (C) 2004 Elsevier B.V. All rights reserved.},
  author       = {Bradoo, Sapna and Svensson, Ingemar and Santos, Jorge and Plieva, Fatima and Mattiasson, Bo and Hatti-Kaul, Rajni},
  issn         = {1873-4863},
  keyword      = {β-Galactosidase,Hexyl-β-galactoside,Octyl-β-galactoside,Transglycosylation,Immobilisation,Cryogel},
  language     = {eng},
  number       = {3},
  pages        = {273--285},
  publisher    = {Elsevier},
  series       = {Journal of Biotechnology},
  title        = {Synthesis of alkylgalactosides using whole cells of Bacillus pseudofirmus species as catalysts},
  url          = {http://dx.doi.org/10.1016/j.jbiotec.2004.03.004},
  volume       = {110},
  year         = {2004},
}