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A biosensor for the analysis of acetonitrile

Håkansson, Kristina LU and Mattiasson, Bo LU (2004) In Biosensors & Bioelectronics 19(7). p.721-726
Abstract
A biosensor for monitoring acetonitrile was constructed. A mixed culture was taken from a degradation reactor and mounted on top of a Clark electrode. The amperometric biosensor was placed in a flow-through cell and integrated into a flow injection system. The metabolic response in terms of oxygen consumption was well correlated to the concentration of acetonitrile in standard solutions. However, when the reaction products, acetic acid and ammonia, were also present, the response was erratic, due to the additional metabolic reaction on acetate. By introducing a hydrophobic barrier it was possible to eliminate the negative influence of these charged products and thus to improve the operational selectivity of the sensor. The biosensor showed... (More)
A biosensor for monitoring acetonitrile was constructed. A mixed culture was taken from a degradation reactor and mounted on top of a Clark electrode. The amperometric biosensor was placed in a flow-through cell and integrated into a flow injection system. The metabolic response in terms of oxygen consumption was well correlated to the concentration of acetonitrile in standard solutions. However, when the reaction products, acetic acid and ammonia, were also present, the response was erratic, due to the additional metabolic reaction on acetate. By introducing a hydrophobic barrier it was possible to eliminate the negative influence of these charged products and thus to improve the operational selectivity of the sensor. The biosensor showed good stability for analysis during at least 6 days and future work will focus on using it for monitoring and control of degradation processes. (C) 2003 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Biosensor, Acetonitrile, Mixed culture, Biodegradation, Clark electrode
in
Biosensors & Bioelectronics
volume
19
issue
7
pages
721 - 726
publisher
Elsevier
external identifiers
  • wos:000188505000011
  • scopus:0347355567
ISSN
1873-4235
DOI
10.1016/j.bios.2003.07.001
language
English
LU publication?
yes
id
9707fe0e-7982-497e-967f-3cbdece5b9b9 (old id 140755)
date added to LUP
2007-07-02 11:17:05
date last changed
2017-07-16 04:04:39
@article{9707fe0e-7982-497e-967f-3cbdece5b9b9,
  abstract     = {A biosensor for monitoring acetonitrile was constructed. A mixed culture was taken from a degradation reactor and mounted on top of a Clark electrode. The amperometric biosensor was placed in a flow-through cell and integrated into a flow injection system. The metabolic response in terms of oxygen consumption was well correlated to the concentration of acetonitrile in standard solutions. However, when the reaction products, acetic acid and ammonia, were also present, the response was erratic, due to the additional metabolic reaction on acetate. By introducing a hydrophobic barrier it was possible to eliminate the negative influence of these charged products and thus to improve the operational selectivity of the sensor. The biosensor showed good stability for analysis during at least 6 days and future work will focus on using it for monitoring and control of degradation processes. (C) 2003 Elsevier B.V. All rights reserved.},
  author       = {Håkansson, Kristina and Mattiasson, Bo},
  issn         = {1873-4235},
  keyword      = {Biosensor,Acetonitrile,Mixed culture,Biodegradation,Clark electrode},
  language     = {eng},
  number       = {7},
  pages        = {721--726},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {A biosensor for the analysis of acetonitrile},
  url          = {http://dx.doi.org/10.1016/j.bios.2003.07.001},
  volume       = {19},
  year         = {2004},
}