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Profiling of internalizing tumor-associated antigens on breast and pancreatic cancer cells by reversed genomics

Fransson, Johan LU ; Ek, Sara LU ; Ellmark, Peter LU ; Söderlind, Eskil LU ; Borrebaeck, Carl LU and Furebring, Christina LU (2004) In Cancer Letters 208(2). p.235-242
Abstract
Human antibodies directed towards functionally associated tumor antigens have great potentials as adjuvant treatment in cancer therapy. Here we describe an efficient subtractive approach to select single chain Fv (scFv) antibodies, specifically binding to unknown rapidly internalizing tumor-associated antigens (TAA) on human breast and pancreatic carcinoma cell lines. After re-engineering the scFv into intact IgG molecules, these fully human antibodies displayed individual binding profiles to TAAs on breast, pancreatic, colorectal and prostate carcinomas, while showing no reactivity to lymphomas. The ability of the selected antibodies to undergo receptor-mediated internalization was verified by confocal microscopy, thus proving our... (More)
Human antibodies directed towards functionally associated tumor antigens have great potentials as adjuvant treatment in cancer therapy. Here we describe an efficient subtractive approach to select single chain Fv (scFv) antibodies, specifically binding to unknown rapidly internalizing tumor-associated antigens (TAA) on human breast and pancreatic carcinoma cell lines. After re-engineering the scFv into intact IgG molecules, these fully human antibodies displayed individual binding profiles to TAAs on breast, pancreatic, colorectal and prostate carcinomas, while showing no reactivity to lymphomas. The ability of the selected antibodies to undergo receptor-mediated internalization was verified by confocal microscopy, thus proving our strategy to provide a unique set of human antibodies, potentially useful in immunotherapy. (C) 2003 Elsevier Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Cancer Letters
volume
208
issue
2
pages
235 - 242
publisher
Elsevier
external identifiers
  • wos:000221682600014
  • pmid:15142683
  • scopus:2442508936
ISSN
1872-7980
DOI
10.1016/j.canlet.2003.11.036
language
English
LU publication?
yes
id
6253bc76-f990-4eb9-9743-0037de259e54 (old id 140797)
date added to LUP
2007-06-28 10:45:12
date last changed
2017-01-01 06:51:46
@article{6253bc76-f990-4eb9-9743-0037de259e54,
  abstract     = {Human antibodies directed towards functionally associated tumor antigens have great potentials as adjuvant treatment in cancer therapy. Here we describe an efficient subtractive approach to select single chain Fv (scFv) antibodies, specifically binding to unknown rapidly internalizing tumor-associated antigens (TAA) on human breast and pancreatic carcinoma cell lines. After re-engineering the scFv into intact IgG molecules, these fully human antibodies displayed individual binding profiles to TAAs on breast, pancreatic, colorectal and prostate carcinomas, while showing no reactivity to lymphomas. The ability of the selected antibodies to undergo receptor-mediated internalization was verified by confocal microscopy, thus proving our strategy to provide a unique set of human antibodies, potentially useful in immunotherapy. (C) 2003 Elsevier Ltd. All rights reserved.},
  author       = {Fransson, Johan and Ek, Sara and Ellmark, Peter and Söderlind, Eskil and Borrebaeck, Carl and Furebring, Christina},
  issn         = {1872-7980},
  language     = {eng},
  number       = {2},
  pages        = {235--242},
  publisher    = {Elsevier},
  series       = {Cancer Letters},
  title        = {Profiling of internalizing tumor-associated antigens on breast and pancreatic cancer cells by reversed genomics},
  url          = {http://dx.doi.org/10.1016/j.canlet.2003.11.036},
  volume       = {208},
  year         = {2004},
}