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Combined pseudo-merohedral twinning, non-crystallographic symmetry and pseudo-translation in a monoclinic crystal form of the gamma delta T-cell ligand T10

Rudolph, MG; Wingren, Christer LU ; Crowley, MP; Chien, YH and Wilson, IA (2004) In Acta Crystallographica. Section D: Biological Crystallography 60(4). p.656-664
Abstract
T10 is a non-classical class Ib-like major histocompatibility complex (MHC) cell-surface antigen which binds directly to certain gammadelta T-cell receptors in the absence of any exogenous and endogenous ligands, such as peculiar lipids or glycolipids. The crystal structure at 2.5 Angstrom resolution of murine T10 was determined by molecular replacement using data from an almost perfectly twinned monoclinic crystal. The space group is P2(1), with unit-cell parameters a=78.2, b=70.0, c=139.2 Angstrom, beta=106.8degrees. Self-rotation function analysis and various intensity statistics revealed the presence of pseudo-merohedral twinning, but these tests underestimated the true twin fraction of alphasimilar or equal to0.46. Native Patterson... (More)
T10 is a non-classical class Ib-like major histocompatibility complex (MHC) cell-surface antigen which binds directly to certain gammadelta T-cell receptors in the absence of any exogenous and endogenous ligands, such as peculiar lipids or glycolipids. The crystal structure at 2.5 Angstrom resolution of murine T10 was determined by molecular replacement using data from an almost perfectly twinned monoclinic crystal. The space group is P2(1), with unit-cell parameters a=78.2, b=70.0, c=139.2 Angstrom, beta=106.8degrees. Self-rotation function analysis and various intensity statistics revealed the presence of pseudo-merohedral twinning, but these tests underestimated the true twin fraction of alphasimilar or equal to0.46. Native Patterson analyses pointed to the presence of pseudo-translation among the four molecules present in the asymmetric unit. Data analysis, structure determination and model refinement are discussed. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Acta Crystallographica. Section D: Biological Crystallography
volume
60
issue
4
pages
656 - 664
publisher
International Union of Crystallography
external identifiers
  • scopus:7944237130
ISSN
1399-0047
DOI
10.1107/S0907444904002239
language
English
LU publication?
yes
id
7d6437d3-b70b-42c2-b1da-df585fc20fe1 (old id 140805)
date added to LUP
2007-07-10 09:47:41
date last changed
2017-01-01 06:48:00
@article{7d6437d3-b70b-42c2-b1da-df585fc20fe1,
  abstract     = {T10 is a non-classical class Ib-like major histocompatibility complex (MHC) cell-surface antigen which binds directly to certain gammadelta T-cell receptors in the absence of any exogenous and endogenous ligands, such as peculiar lipids or glycolipids. The crystal structure at 2.5 Angstrom resolution of murine T10 was determined by molecular replacement using data from an almost perfectly twinned monoclinic crystal. The space group is P2(1), with unit-cell parameters a=78.2, b=70.0, c=139.2 Angstrom, beta=106.8degrees. Self-rotation function analysis and various intensity statistics revealed the presence of pseudo-merohedral twinning, but these tests underestimated the true twin fraction of alphasimilar or equal to0.46. Native Patterson analyses pointed to the presence of pseudo-translation among the four molecules present in the asymmetric unit. Data analysis, structure determination and model refinement are discussed.},
  author       = {Rudolph, MG and Wingren, Christer and Crowley, MP and Chien, YH and Wilson, IA},
  issn         = {1399-0047},
  language     = {eng},
  number       = {4},
  pages        = {656--664},
  publisher    = {International Union of Crystallography},
  series       = {Acta Crystallographica. Section D: Biological Crystallography},
  title        = {Combined pseudo-merohedral twinning, non-crystallographic symmetry and pseudo-translation in a monoclinic crystal form of the gamma delta T-cell ligand T10},
  url          = {http://dx.doi.org/10.1107/S0907444904002239},
  volume       = {60},
  year         = {2004},
}