Gene expression profiling of Escherichia coli expressing double Vitreoscilla haemoglobin
(2004) In Journal of Biotechnology 114(1-2). p.107-120- Abstract
- In a recent investigation, expression of a double Vitreoscilla haemoglobin (two fused VHb molecules) in Escherichia coli grown in shake flasks resulted in higher final cell density and considerably higher levels of ribosomes and tRNA. In this study, we have investigated the E. coli transcriptome in cells expressing native VHb, double VHb and control cells lacking VHb by hybridising mRNA from the different constructs to high-density oligonucleotide, arrays. Within the 95% confidence interval, 4 and 5% of all detected genes in native VHb cells were up- and down-regulated, respectively; in double VHb cells the corresponding numbers were 6 and 10%, respectively. Dividing the data into different functional groups revealed that genes involved in... (More)
- In a recent investigation, expression of a double Vitreoscilla haemoglobin (two fused VHb molecules) in Escherichia coli grown in shake flasks resulted in higher final cell density and considerably higher levels of ribosomes and tRNA. In this study, we have investigated the E. coli transcriptome in cells expressing native VHb, double VHb and control cells lacking VHb by hybridising mRNA from the different constructs to high-density oligonucleotide, arrays. Within the 95% confidence interval, 4 and 5% of all detected genes in native VHb cells were up- and down-regulated, respectively; in double VHb cells the corresponding numbers were 6 and 10%, respectively. Dividing the data into different functional groups revealed that genes involved in energy metabolism, central intermediary metabolism and cell processes were the most affected at the mRNA level. Particularly, the up-regulation of genes involved in translation and posttranslational modification observed in double VHb cells demonstrates a strong relationship between the regulation of ribosomal genes and the actual number of ribosomes. (C) 2004 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/140979
- author
- Roos, Viktoria LU ; Andersson, Charlotte LU and Bülow, Leif LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Biotechnology
- volume
- 114
- issue
- 1-2
- pages
- 107 - 120
- publisher
- Elsevier
- external identifiers
-
- wos:000224598000012
- pmid:15464604
- scopus:4644373188
- ISSN
- 1873-4863
- DOI
- 10.1016/j.jbiotec.2004.07.002
- language
- English
- LU publication?
- yes
- id
- 2d03151a-a65b-43b1-9019-9f9423162ad3 (old id 140979)
- date added to LUP
- 2016-04-01 12:22:31
- date last changed
- 2022-02-18 21:38:46
@article{2d03151a-a65b-43b1-9019-9f9423162ad3,
abstract = {{In a recent investigation, expression of a double Vitreoscilla haemoglobin (two fused VHb molecules) in Escherichia coli grown in shake flasks resulted in higher final cell density and considerably higher levels of ribosomes and tRNA. In this study, we have investigated the E. coli transcriptome in cells expressing native VHb, double VHb and control cells lacking VHb by hybridising mRNA from the different constructs to high-density oligonucleotide, arrays. Within the 95% confidence interval, 4 and 5% of all detected genes in native VHb cells were up- and down-regulated, respectively; in double VHb cells the corresponding numbers were 6 and 10%, respectively. Dividing the data into different functional groups revealed that genes involved in energy metabolism, central intermediary metabolism and cell processes were the most affected at the mRNA level. Particularly, the up-regulation of genes involved in translation and posttranslational modification observed in double VHb cells demonstrates a strong relationship between the regulation of ribosomal genes and the actual number of ribosomes. (C) 2004 Elsevier B.V. All rights reserved.}},
author = {{Roos, Viktoria and Andersson, Charlotte and Bülow, Leif}},
issn = {{1873-4863}},
language = {{eng}},
number = {{1-2}},
pages = {{107--120}},
publisher = {{Elsevier}},
series = {{Journal of Biotechnology}},
title = {{Gene expression profiling of Escherichia coli expressing double Vitreoscilla haemoglobin}},
url = {{http://dx.doi.org/10.1016/j.jbiotec.2004.07.002}},
doi = {{10.1016/j.jbiotec.2004.07.002}},
volume = {{114}},
year = {{2004}},
}