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Electrochemical study of the XNA on Gold (TM) microarray

Liang, Z P; Xie, W H; Zhang, X X; Chen, J; Zhang, X N; Danielsson, Bengt LU ; Mecklenburg, Michael and Xie, Bin LU (2004) In Biosensors & Bioelectronics 20(2). p.211-216
Abstract
A novel electrode array was developed based on the XNA on Gold(TM) microarray platform. The platform combines self-assembling monolayers, thick film patterning and streptavidin based immobilization to provide a robust, versatile platform capable of analysing virtually any biomolecule including nucleic acids, proteins, carbohydrates and lipids. Electrochemical analysis of the self-assembling monolayer/streptavidin (SAMS) XNA on Gold(TM) coating revealed that the ferrocene redox current for the SAMS modified electrode was greater than that with a bare Gold(TM) electrode. The electrochemical reaction of K4Fe(CN)(6) was inhibited by the SAMS coating, but was reactivated upon addition of ferrocene. These results indicate that ferrocene is... (More)
A novel electrode array was developed based on the XNA on Gold(TM) microarray platform. The platform combines self-assembling monolayers, thick film patterning and streptavidin based immobilization to provide a robust, versatile platform capable of analysing virtually any biomolecule including nucleic acids, proteins, carbohydrates and lipids. Electrochemical analysis of the self-assembling monolayer/streptavidin (SAMS) XNA on Gold(TM) coating revealed that the ferrocene redox current for the SAMS modified electrode was greater than that with a bare Gold(TM) electrode. The electrochemical reaction of K4Fe(CN)(6) was inhibited by the SAMS coating, but was reactivated upon addition of ferrocene. These results indicate that ferrocene is involved as a mediator in the electron transfer of K4Fe(CN)(6) to the SAMS modified electrode. Addition of DNA to the SAMS resulted in only a minor change in the electrochemical signal, indicating that XNA on Gold(TM) can be used for electrochemical based bioanalysis. After cycling a SAMS electrode 50 times, no signs of deterioration were detected showing that coating has excellent stability. In addition to the biosensing applications, the scheme provides a non-invasive method for accessing the quality of the SAMS coatings which is of industrial interest. These studies show that the XNA on Gold(TM) microarray platform can be used for electrochemical studies, thus providing an additional alternative for developing multianalyte biosensors as well as expanding the range of detection methods available for microarray analysis. (C) 2004 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
DNA, Self-assembled monolayers, Microarray, XNA on Gold™, Biochip, Streptavidin, Electrochemical detection, Modified electrodes, K4Fe(CN)6·3H2O, Ferrocene
in
Biosensors & Bioelectronics
volume
20
issue
2
pages
211 - 216
publisher
Elsevier
external identifiers
  • wos:000224034400011
  • pmid:15308224
  • scopus:4043060605
ISSN
1873-4235
DOI
10.1016/j.bios.2004.02.026
language
English
LU publication?
yes
id
936475df-ab3b-47b3-b92d-c97f4ce00f1c (old id 140988)
date added to LUP
2007-07-16 09:58:24
date last changed
2017-01-01 06:47:34
@article{936475df-ab3b-47b3-b92d-c97f4ce00f1c,
  abstract     = {A novel electrode array was developed based on the XNA on Gold(TM) microarray platform. The platform combines self-assembling monolayers, thick film patterning and streptavidin based immobilization to provide a robust, versatile platform capable of analysing virtually any biomolecule including nucleic acids, proteins, carbohydrates and lipids. Electrochemical analysis of the self-assembling monolayer/streptavidin (SAMS) XNA on Gold(TM) coating revealed that the ferrocene redox current for the SAMS modified electrode was greater than that with a bare Gold(TM) electrode. The electrochemical reaction of K4Fe(CN)(6) was inhibited by the SAMS coating, but was reactivated upon addition of ferrocene. These results indicate that ferrocene is involved as a mediator in the electron transfer of K4Fe(CN)(6) to the SAMS modified electrode. Addition of DNA to the SAMS resulted in only a minor change in the electrochemical signal, indicating that XNA on Gold(TM) can be used for electrochemical based bioanalysis. After cycling a SAMS electrode 50 times, no signs of deterioration were detected showing that coating has excellent stability. In addition to the biosensing applications, the scheme provides a non-invasive method for accessing the quality of the SAMS coatings which is of industrial interest. These studies show that the XNA on Gold(TM) microarray platform can be used for electrochemical studies, thus providing an additional alternative for developing multianalyte biosensors as well as expanding the range of detection methods available for microarray analysis. (C) 2004 Elsevier B.V. All rights reserved.},
  author       = {Liang, Z P and Xie, W H and Zhang, X X and Chen, J and Zhang, X N and Danielsson, Bengt and Mecklenburg, Michael and Xie, Bin},
  issn         = {1873-4235},
  keyword      = {DNA,Self-assembled monolayers,Microarray,XNA on Gold™,Biochip,Streptavidin,Electrochemical detection,Modified electrodes,K4Fe(CN)6·3H2O,Ferrocene},
  language     = {eng},
  number       = {2},
  pages        = {211--216},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {Electrochemical study of the XNA on Gold (TM) microarray},
  url          = {http://dx.doi.org/10.1016/j.bios.2004.02.026},
  volume       = {20},
  year         = {2004},
}