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Novel peptide surface for reversible immobilization of concanavalin A

Masarova, Jana LU ; Dey, Estera LU ; Carlsson, J and Danielsson, Bengt LU (2004) In Journal of Biochemical and Biophysical Methods1979-01-01+01:002008-01-01+01:00 60(2). p.163-170
Abstract
Concanavalin A (Con A) was spontaneously adsorbed on polymyxin B surface. This peptide-lectin interaction was strong, K-D = 1-9 X 10(-10), based predominantly on creation of hydrophobic bonds, and was completely reversible. Concanavalin A on polymyxin B (PmB) retained higher binding capacity for yeast mannan, compared with covalently immobilized lectin. Kinetics of mannan-concanavalin A interaction were significantly different in dependence on type of concanavalin A immobilization. (C) 2004 Elsevier B.V All rights reserved.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Immobilization, Hydrophobic interaction, Concanavalin A, Polymyxin B, Surface plasmon resonance
in
Journal of Biochemical and Biophysical Methods1979-01-01+01:002008-01-01+01:00
volume
60
issue
2
pages
163 - 170
publisher
Elsevier
external identifiers
  • wos:000223463400006
  • scopus:3242713049
ISSN
0165-022X
DOI
10.1016/j.jbbm.2004.05.005
language
English
LU publication?
yes
id
e7e463ee-0044-4384-8c2a-2d53f86d3b46 (old id 140998)
date added to LUP
2007-07-16 10:01:29
date last changed
2017-11-30 16:12:55
@article{e7e463ee-0044-4384-8c2a-2d53f86d3b46,
  abstract     = {Concanavalin A (Con A) was spontaneously adsorbed on polymyxin B surface. This peptide-lectin interaction was strong, K-D = 1-9 X 10(-10), based predominantly on creation of hydrophobic bonds, and was completely reversible. Concanavalin A on polymyxin B (PmB) retained higher binding capacity for yeast mannan, compared with covalently immobilized lectin. Kinetics of mannan-concanavalin A interaction were significantly different in dependence on type of concanavalin A immobilization. (C) 2004 Elsevier B.V All rights reserved.},
  author       = {Masarova, Jana and Dey, Estera and Carlsson, J and Danielsson, Bengt},
  issn         = {0165-022X},
  keyword      = {Immobilization,Hydrophobic interaction,Concanavalin A,Polymyxin B,Surface plasmon resonance},
  language     = {eng},
  number       = {2},
  pages        = {163--170},
  publisher    = {Elsevier},
  series       = {Journal of Biochemical and Biophysical Methods1979-01-01+01:002008-01-01+01:00},
  title        = {Novel peptide surface for reversible immobilization of concanavalin A},
  url          = {http://dx.doi.org/10.1016/j.jbbm.2004.05.005},
  volume       = {60},
  year         = {2004},
}