Novel peptide surface for reversible immobilization of concanavalin A
(2004) In Journal of Biochemical and Biophysical Methods 60(2). p.163-170- Abstract
- Concanavalin A (Con A) was spontaneously adsorbed on polymyxin B surface. This peptide-lectin interaction was strong, K-D = 1-9 X 10(-10), based predominantly on creation of hydrophobic bonds, and was completely reversible. Concanavalin A on polymyxin B (PmB) retained higher binding capacity for yeast mannan, compared with covalently immobilized lectin. Kinetics of mannan-concanavalin A interaction were significantly different in dependence on type of concanavalin A immobilization. (C) 2004 Elsevier B.V All rights reserved.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/140998
- author
- Masarova, Jana LU ; Dey, Estera LU ; Carlsson, J and Danielsson, Bengt LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Immobilization, Hydrophobic interaction, Concanavalin A, Polymyxin B, Surface plasmon resonance
- in
- Journal of Biochemical and Biophysical Methods
- volume
- 60
- issue
- 2
- pages
- 163 - 170
- publisher
- Elsevier
- external identifiers
-
- wos:000223463400006
- scopus:3242713049
- ISSN
- 0165-022X
- DOI
- 10.1016/j.jbbm.2004.05.005
- language
- English
- LU publication?
- yes
- id
- e7e463ee-0044-4384-8c2a-2d53f86d3b46 (old id 140998)
- date added to LUP
- 2016-04-01 15:55:01
- date last changed
- 2022-01-28 08:00:36
@article{e7e463ee-0044-4384-8c2a-2d53f86d3b46, abstract = {{Concanavalin A (Con A) was spontaneously adsorbed on polymyxin B surface. This peptide-lectin interaction was strong, K-D = 1-9 X 10(-10), based predominantly on creation of hydrophobic bonds, and was completely reversible. Concanavalin A on polymyxin B (PmB) retained higher binding capacity for yeast mannan, compared with covalently immobilized lectin. Kinetics of mannan-concanavalin A interaction were significantly different in dependence on type of concanavalin A immobilization. (C) 2004 Elsevier B.V All rights reserved.}}, author = {{Masarova, Jana and Dey, Estera and Carlsson, J and Danielsson, Bengt}}, issn = {{0165-022X}}, keywords = {{Immobilization; Hydrophobic interaction; Concanavalin A; Polymyxin B; Surface plasmon resonance}}, language = {{eng}}, number = {{2}}, pages = {{163--170}}, publisher = {{Elsevier}}, series = {{Journal of Biochemical and Biophysical Methods}}, title = {{Novel peptide surface for reversible immobilization of concanavalin A}}, url = {{http://dx.doi.org/10.1016/j.jbbm.2004.05.005}}, doi = {{10.1016/j.jbbm.2004.05.005}}, volume = {{60}}, year = {{2004}}, }