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Application of dummy molecularly imprinted solid-phase extraction in the analysis of cyproheptadine in bovine urine.

Feás, Xesús; Ye, Lei LU ; Regal, Patricia; Fente, Cristina A; Hosseini, Seyed V and Cepeda, Alberto (2009) In Journal of Separation Science 32(10). p.1740-1747
Abstract
Due to the difficulty of monitoring trace levels of cyproheptadine (CYP) residues in complicated biological matrices, specific adsorption materials for the preconcentration and clean-up of CYP are indispensable. In this work, CYP was extracted from urine using dummy molecularly imprinted SPE (DMISPE) to avoid leakage of the imprinting molecules during the desorption phase. For synthesis of DMISPE, azatadine (AZA) was employed as the dummy template, methacrylic acid (MAA) as the monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linker, 2,2'-azobis(2-methylpropionitrile) (AIBN) as the initiator, and dichloromethane as the porogen solvent. An LC-MS/MS method was used to analyze CYP. Two MRM (multiple reaction monitoring)... (More)
Due to the difficulty of monitoring trace levels of cyproheptadine (CYP) residues in complicated biological matrices, specific adsorption materials for the preconcentration and clean-up of CYP are indispensable. In this work, CYP was extracted from urine using dummy molecularly imprinted SPE (DMISPE) to avoid leakage of the imprinting molecules during the desorption phase. For synthesis of DMISPE, azatadine (AZA) was employed as the dummy template, methacrylic acid (MAA) as the monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linker, 2,2'-azobis(2-methylpropionitrile) (AIBN) as the initiator, and dichloromethane as the porogen solvent. An LC-MS/MS method was used to analyze CYP. Two MRM (multiple reaction monitoring) transitions for each analyte were monitored using diphenylpyraline hydrochloride (DPP.HCl), which was used as an internal standard. The advantages of DMISPE include obtaining less complex chromatograms and reducing ion suppression in ESI. The process efficiencies for DMISPE and SPE were 80% and 12%, respectively. In addition, the demonstrated reusability of the DMISPE cartridges is an advantage compared with single-use SPE cartridges or immunoaffinity materials. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Separation Science
volume
32
issue
10
pages
1740 - 1747
publisher
John Wiley & Sons
external identifiers
  • wos:000266508700025
  • pmid:19472275
  • scopus:67649905032
ISSN
1615-9314
DOI
10.1002/jssc.200800726
language
English
LU publication?
yes
id
b9b4c0b4-7025-418b-b6dc-5d48ceddac67 (old id 1411983)
date added to LUP
2009-06-11 09:05:01
date last changed
2017-01-01 04:38:11
@article{b9b4c0b4-7025-418b-b6dc-5d48ceddac67,
  abstract     = {Due to the difficulty of monitoring trace levels of cyproheptadine (CYP) residues in complicated biological matrices, specific adsorption materials for the preconcentration and clean-up of CYP are indispensable. In this work, CYP was extracted from urine using dummy molecularly imprinted SPE (DMISPE) to avoid leakage of the imprinting molecules during the desorption phase. For synthesis of DMISPE, azatadine (AZA) was employed as the dummy template, methacrylic acid (MAA) as the monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linker, 2,2'-azobis(2-methylpropionitrile) (AIBN) as the initiator, and dichloromethane as the porogen solvent. An LC-MS/MS method was used to analyze CYP. Two MRM (multiple reaction monitoring) transitions for each analyte were monitored using diphenylpyraline hydrochloride (DPP.HCl), which was used as an internal standard. The advantages of DMISPE include obtaining less complex chromatograms and reducing ion suppression in ESI. The process efficiencies for DMISPE and SPE were 80% and 12%, respectively. In addition, the demonstrated reusability of the DMISPE cartridges is an advantage compared with single-use SPE cartridges or immunoaffinity materials.},
  author       = {Feás, Xesús and Ye, Lei and Regal, Patricia and Fente, Cristina A and Hosseini, Seyed V and Cepeda, Alberto},
  issn         = {1615-9314},
  language     = {eng},
  number       = {10},
  pages        = {1740--1747},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Separation Science},
  title        = {Application of dummy molecularly imprinted solid-phase extraction in the analysis of cyproheptadine in bovine urine.},
  url          = {http://dx.doi.org/10.1002/jssc.200800726},
  volume       = {32},
  year         = {2009},
}