Advanced

Community Structure Evolution and Enrichment of Glycogen-Accumulating Organisms Producing Polyhydroxyalkanoates from Fermented Molasses

Pisco, Ana R.; Bengtsson, Simon LU ; Werker, Alan; Reis, Maria A. M. and Lemos, Paulo C. (2009) In Applied and Environmental Microbiology 75(14). p.4676-4686
Abstract
An open mixed culture was enriched with glycogen-accumulating organisms (GAOs) by using a sequencing batch reactor and treating an agroindustrial waste (sugar cane molasses) under cyclic anaerobic-aerobic conditions. Over a 1-year operating period, the culture exhibited a very stable GAO phenotype with an average polyhydroxyalkanoate (PHA) content of 17% total suspended solids. However, the GAO microbial community evolved over the course of operation to a culture exhibiting unusual characteristics in producing PHAs comprised of short-chain-length monomers, namely, 3-hydroxybutyrate, 3-hydroxy-2- methylbutyrate, 3-hydroxyvalerate, and 3-hydroxy-2- methylvalerate, and also, up to 31 mol% of the medium-chain-length (MCL) monomer... (More)
An open mixed culture was enriched with glycogen-accumulating organisms (GAOs) by using a sequencing batch reactor and treating an agroindustrial waste (sugar cane molasses) under cyclic anaerobic-aerobic conditions. Over a 1-year operating period, the culture exhibited a very stable GAO phenotype with an average polyhydroxyalkanoate (PHA) content of 17% total suspended solids. However, the GAO microbial community evolved over the course of operation to a culture exhibiting unusual characteristics in producing PHAs comprised of short-chain-length monomers, namely, 3-hydroxybutyrate, 3-hydroxy-2- methylbutyrate, 3-hydroxyvalerate, and 3-hydroxy-2- methylvalerate, and also, up to 31 mol% of the medium-chain-length (MCL) monomer 3-hydroxyhexanoate (3HHx). Microbial community analysis by fluorescence in situ hybridization revealed a concurrent long-term drift in the GAO community balance, from mainly "Candidatus Competibacter phosphatis" to mainly Defluviicoccus vanus-related organisms. The production of 3HHx was confirmed by C-13 nuclear magnetic resonance (NMR) and appeared to be related to the increased presence of D. vanus-related GAOs. These results suggest a broadened spectrum of material, chemical, and mechanical properties that can be achieved for biopolymers produced by open mixed cultures from fermented waste. The increased spectrum of polymer properties brings a wider scope of potential applications. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied and Environmental Microbiology
volume
75
issue
14
pages
4676 - 4686
publisher
American Society for Microbiology
external identifiers
  • pmid:19465533
  • scopus:67650498162
  • wos:000267847000003
ISSN
0099-2240
DOI
10.1128/AEM.02486-08
language
English
LU publication?
yes
id
6ba5aa5e-e165-4cb1-b21c-57e535223b9f (old id 1412059)
date added to LUP
2009-08-19 08:36:59
date last changed
2017-12-10 03:38:23
@article{6ba5aa5e-e165-4cb1-b21c-57e535223b9f,
  abstract     = {An open mixed culture was enriched with glycogen-accumulating organisms (GAOs) by using a sequencing batch reactor and treating an agroindustrial waste (sugar cane molasses) under cyclic anaerobic-aerobic conditions. Over a 1-year operating period, the culture exhibited a very stable GAO phenotype with an average polyhydroxyalkanoate (PHA) content of 17% total suspended solids. However, the GAO microbial community evolved over the course of operation to a culture exhibiting unusual characteristics in producing PHAs comprised of short-chain-length monomers, namely, 3-hydroxybutyrate, 3-hydroxy-2- methylbutyrate, 3-hydroxyvalerate, and 3-hydroxy-2- methylvalerate, and also, up to 31 mol% of the medium-chain-length (MCL) monomer 3-hydroxyhexanoate (3HHx). Microbial community analysis by fluorescence in situ hybridization revealed a concurrent long-term drift in the GAO community balance, from mainly "Candidatus Competibacter phosphatis" to mainly Defluviicoccus vanus-related organisms. The production of 3HHx was confirmed by C-13 nuclear magnetic resonance (NMR) and appeared to be related to the increased presence of D. vanus-related GAOs. These results suggest a broadened spectrum of material, chemical, and mechanical properties that can be achieved for biopolymers produced by open mixed cultures from fermented waste. The increased spectrum of polymer properties brings a wider scope of potential applications.},
  author       = {Pisco, Ana R. and Bengtsson, Simon and Werker, Alan and Reis, Maria A. M. and Lemos, Paulo C.},
  issn         = {0099-2240},
  language     = {eng},
  number       = {14},
  pages        = {4676--4686},
  publisher    = {American Society for Microbiology},
  series       = {Applied and Environmental Microbiology},
  title        = {Community Structure Evolution and Enrichment of Glycogen-Accumulating Organisms Producing Polyhydroxyalkanoates from Fermented Molasses},
  url          = {http://dx.doi.org/10.1128/AEM.02486-08},
  volume       = {75},
  year         = {2009},
}