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Design of high-density antibody microarrays for disease proteomics: Key technological issues.

Borrebaeck, Carl LU and Wingren, Christer LU (2009) In Journal of Proteomics2008-01-01+01:00 72(6). p.928-935
Abstract
Antibody-based microarray is a novel proteomic technology setting a new standard for molecular profiling of non-fractionated complex proteomes. The first generation of antibody microarrays has already demonstrated its potential for generating detailed protein expression profiles, or protein atlases, of human body fluids in health and disease, paving the way for new discoveries within the field of disease proteomics. The process of designing highly miniaturized, high-density and high-performing antibody microarray set-ups have, however, proven to be challenging. In this mini-review we discuss key technological issues that must be addressed in a cross-disciplinary manner before true global proteome analysis can be performed using antibody... (More)
Antibody-based microarray is a novel proteomic technology setting a new standard for molecular profiling of non-fractionated complex proteomes. The first generation of antibody microarrays has already demonstrated its potential for generating detailed protein expression profiles, or protein atlases, of human body fluids in health and disease, paving the way for new discoveries within the field of disease proteomics. The process of designing highly miniaturized, high-density and high-performing antibody microarray set-ups have, however, proven to be challenging. In this mini-review we discuss key technological issues that must be addressed in a cross-disciplinary manner before true global proteome analysis can be performed using antibody microarrays. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Proteomics2008-01-01+01:00
volume
72
issue
6
pages
928 - 935
publisher
Elsevier
external identifiers
  • wos:000269429800003
  • pmid:19457338
  • scopus:67651161904
ISSN
1874-3919
DOI
10.1016/j.jprot.2009.01.027
project
CREATE Health
language
English
LU publication?
yes
id
e66eb26c-d0d8-47f8-9829-a39d99ae2ca1 (old id 1412094)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19457338?dopt=Abstract
date added to LUP
2009-06-03 15:20:21
date last changed
2017-11-26 03:24:38
@article{e66eb26c-d0d8-47f8-9829-a39d99ae2ca1,
  abstract     = {Antibody-based microarray is a novel proteomic technology setting a new standard for molecular profiling of non-fractionated complex proteomes. The first generation of antibody microarrays has already demonstrated its potential for generating detailed protein expression profiles, or protein atlases, of human body fluids in health and disease, paving the way for new discoveries within the field of disease proteomics. The process of designing highly miniaturized, high-density and high-performing antibody microarray set-ups have, however, proven to be challenging. In this mini-review we discuss key technological issues that must be addressed in a cross-disciplinary manner before true global proteome analysis can be performed using antibody microarrays.},
  author       = {Borrebaeck, Carl and Wingren, Christer},
  issn         = {1874-3919},
  language     = {eng},
  number       = {6},
  pages        = {928--935},
  publisher    = {Elsevier},
  series       = {Journal of Proteomics2008-01-01+01:00},
  title        = {Design of high-density antibody microarrays for disease proteomics: Key technological issues.},
  url          = {http://dx.doi.org/10.1016/j.jprot.2009.01.027},
  volume       = {72},
  year         = {2009},
}