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Complete high-density lipoproteins in nanoparticle corona.

Hellstrand, Erik LU ; Lynch, Iseult; Andersson, Astra LU ; Drakenberg, Torbjörn LU ; Dahlbäck, Björn LU ; Dawson, Kenneth A; Linse, Sara LU and Cedervall, Tommy LU (2009) In The FEBS Journal 276(12). p.3372-3381
Abstract
In a biological environment, nanoparticles immediately become covered by an evolving corona of biomolecules, which gives a biological identity to the nanoparticle and determines its biological impact and fate. Previous efforts at describing the corona have concerned only its protein content. Here, for the first time, we show, using size exclusion chromatography, NMR, and pull-down experiments, that copolymer nanoparticles bind cholesterol, triglycerides and phospholipids from human plasma, and that the binding reaches saturation. The lipid and protein binding patterns correspond closely with the composition of high-density lipoprotein (HDL). By using fractionated lipoproteins, we show that HDL binds to copolymer nanoparticles with much... (More)
In a biological environment, nanoparticles immediately become covered by an evolving corona of biomolecules, which gives a biological identity to the nanoparticle and determines its biological impact and fate. Previous efforts at describing the corona have concerned only its protein content. Here, for the first time, we show, using size exclusion chromatography, NMR, and pull-down experiments, that copolymer nanoparticles bind cholesterol, triglycerides and phospholipids from human plasma, and that the binding reaches saturation. The lipid and protein binding patterns correspond closely with the composition of high-density lipoprotein (HDL). By using fractionated lipoproteins, we show that HDL binds to copolymer nanoparticles with much higher specificity than other lipoproteins, probably mediated by apolipoprotein A-I. Together with the previously identified protein binding patterns in the corona, our results imply that copolymer nanoparticles bind complete HDL complexes, and may be recognized by living systems as HDL complexes, opening up these transport pathways to nanoparticles. Apolipoproteins have been identified as binding to many other nanoparticles, suggesting that lipid and lipoprotein binding is a general feature of nanoparticles under physiological conditions. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
The FEBS Journal
volume
276
issue
12
pages
3372 - 3381
publisher
Federation of European Neuroscience Societies and Blackwell Publishing Ltd
external identifiers
  • wos:000266267800017
  • pmid:19438706
  • scopus:66249116656
ISSN
1742-464X
DOI
10.1111/j.1742-4658.2009.07062.x
language
English
LU publication?
yes
id
8daf8d29-d5bd-481d-a671-7c49929830c2 (old id 1412324)
date added to LUP
2009-06-23 17:11:46
date last changed
2017-11-05 03:43:19
@article{8daf8d29-d5bd-481d-a671-7c49929830c2,
  abstract     = {In a biological environment, nanoparticles immediately become covered by an evolving corona of biomolecules, which gives a biological identity to the nanoparticle and determines its biological impact and fate. Previous efforts at describing the corona have concerned only its protein content. Here, for the first time, we show, using size exclusion chromatography, NMR, and pull-down experiments, that copolymer nanoparticles bind cholesterol, triglycerides and phospholipids from human plasma, and that the binding reaches saturation. The lipid and protein binding patterns correspond closely with the composition of high-density lipoprotein (HDL). By using fractionated lipoproteins, we show that HDL binds to copolymer nanoparticles with much higher specificity than other lipoproteins, probably mediated by apolipoprotein A-I. Together with the previously identified protein binding patterns in the corona, our results imply that copolymer nanoparticles bind complete HDL complexes, and may be recognized by living systems as HDL complexes, opening up these transport pathways to nanoparticles. Apolipoproteins have been identified as binding to many other nanoparticles, suggesting that lipid and lipoprotein binding is a general feature of nanoparticles under physiological conditions.},
  author       = {Hellstrand, Erik and Lynch, Iseult and Andersson, Astra and Drakenberg, Torbjörn and Dahlbäck, Björn and Dawson, Kenneth A and Linse, Sara and Cedervall, Tommy},
  issn         = {1742-464X},
  language     = {eng},
  number       = {12},
  pages        = {3372--3381},
  publisher    = {Federation of European Neuroscience Societies and Blackwell Publishing Ltd},
  series       = {The FEBS Journal},
  title        = {Complete high-density lipoproteins in nanoparticle corona.},
  url          = {http://dx.doi.org/10.1111/j.1742-4658.2009.07062.x},
  volume       = {276},
  year         = {2009},
}