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Increased angiotensin II type 1 receptor expression in temporal arteries from patients with giant cell arteritis.

Dimitrijevic, Ivan LU ; Malmsjö, Malin LU ; Andersson, Christina LU ; Rissler, Pehr LU and Edvinsson, Lars LU (2009) In Ophthalmology 116(5). p.990-996
Abstract
PURPOSE: Currently, giant cell arteritis (GCA) is primarily treated with corticosteroids or immunomodulating agents, but there is interest in identifying other noncorticosteroid alternatives. Similarities exist in the injury pathways between GCA and atherosclerosis. Angiotensin II is a vasoactive peptide involved in vessel inflammation during atherosclerosis, and angiotensin II receptor inhibitors are effective in preventing atherosclerosis. The present study was performed to elucidate the role of angiotensin type 1 (AT(1)) and type 2 (AT(2)) receptors in GCA. DESIGN: Experimental retrospective immunohistochemical study of temporal arteries using archival formalin-fixed, paraffin-embedded tissue. PARTICIPANTS: Ten patients with GCA and 10... (More)
PURPOSE: Currently, giant cell arteritis (GCA) is primarily treated with corticosteroids or immunomodulating agents, but there is interest in identifying other noncorticosteroid alternatives. Similarities exist in the injury pathways between GCA and atherosclerosis. Angiotensin II is a vasoactive peptide involved in vessel inflammation during atherosclerosis, and angiotensin II receptor inhibitors are effective in preventing atherosclerosis. The present study was performed to elucidate the role of angiotensin type 1 (AT(1)) and type 2 (AT(2)) receptors in GCA. DESIGN: Experimental retrospective immunohistochemical study of temporal arteries using archival formalin-fixed, paraffin-embedded tissue. PARTICIPANTS: Ten patients with GCA and 10 control patients, who were clinically suspected of having GCA but were diagnosed as not having GCA, were included. METHODS: Immunohistochemistry, using anti-AT(1) and anti-AT(2) antibodies, was performed on formalin-fixed and paraffin-embedded temporal arteries. MAIN OUTCOME MEASURES: AT(1) and AT(2) receptor immunostaining intensity was quantified. RESULTS: Hematoxylin-eosin-stained sections of temporal arteries from patients with GCA showed intimal hyperplasia, internal elastic lamina degeneration, and band-shaped infiltrates of inflammatory cells, including lymphocytes, histocytes, and multinucleated giant cells. AT(1) receptor staining was primarily observed in the medial layer of the temporal arteries and was higher in the patients with GCA than in the control patients. This was a result of increased AT(1) receptor immunostaining of both vascular smooth muscle cells and infiltrating inflammatory cells. Only faint immunostaining was seen for AT(2) receptors, primarily in the endothelial cells, and to a lesser extent on the smooth muscle cells. Immunostaining with antibodies for the AT(2) receptor was similar in the patients with GCA and in controls. CONCLUSIONS: These results suggest that AT(1) receptors play a role in the development of GCA. Inhibition of the angiotensin system may thus provide a noncorticosteroid alternative for the treatment of GCA. FINANCIAL DISCLOSURE(S): The author(s) have no proprietary or commercial interest in any materials discussed in this article. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
Temporal Arteries: metabolism, Type 2: metabolism, Angiotensin, Receptor, Type 1: metabolism, Vascular: metabolism, Smooth, Muscle, Endothelium, Giant Cell Arteritis: metabolism
in
Ophthalmology
volume
116
issue
5
pages
990 - 996
publisher
Elsevier
external identifiers
  • wos:000270907500025
  • pmid:19410957
  • scopus:65349092825
ISSN
1549-4713
DOI
10.1016/j.ophtha.2008.12.021
language
English
LU publication?
yes
id
f19e4d2d-9a36-4969-9782-a79451d744a6 (old id 1412765)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19410957?dopt=Abstract
date added to LUP
2009-06-01 11:00:02
date last changed
2017-02-12 04:19:21
@article{f19e4d2d-9a36-4969-9782-a79451d744a6,
  abstract     = {PURPOSE: Currently, giant cell arteritis (GCA) is primarily treated with corticosteroids or immunomodulating agents, but there is interest in identifying other noncorticosteroid alternatives. Similarities exist in the injury pathways between GCA and atherosclerosis. Angiotensin II is a vasoactive peptide involved in vessel inflammation during atherosclerosis, and angiotensin II receptor inhibitors are effective in preventing atherosclerosis. The present study was performed to elucidate the role of angiotensin type 1 (AT(1)) and type 2 (AT(2)) receptors in GCA. DESIGN: Experimental retrospective immunohistochemical study of temporal arteries using archival formalin-fixed, paraffin-embedded tissue. PARTICIPANTS: Ten patients with GCA and 10 control patients, who were clinically suspected of having GCA but were diagnosed as not having GCA, were included. METHODS: Immunohistochemistry, using anti-AT(1) and anti-AT(2) antibodies, was performed on formalin-fixed and paraffin-embedded temporal arteries. MAIN OUTCOME MEASURES: AT(1) and AT(2) receptor immunostaining intensity was quantified. RESULTS: Hematoxylin-eosin-stained sections of temporal arteries from patients with GCA showed intimal hyperplasia, internal elastic lamina degeneration, and band-shaped infiltrates of inflammatory cells, including lymphocytes, histocytes, and multinucleated giant cells. AT(1) receptor staining was primarily observed in the medial layer of the temporal arteries and was higher in the patients with GCA than in the control patients. This was a result of increased AT(1) receptor immunostaining of both vascular smooth muscle cells and infiltrating inflammatory cells. Only faint immunostaining was seen for AT(2) receptors, primarily in the endothelial cells, and to a lesser extent on the smooth muscle cells. Immunostaining with antibodies for the AT(2) receptor was similar in the patients with GCA and in controls. CONCLUSIONS: These results suggest that AT(1) receptors play a role in the development of GCA. Inhibition of the angiotensin system may thus provide a noncorticosteroid alternative for the treatment of GCA. FINANCIAL DISCLOSURE(S): The author(s) have no proprietary or commercial interest in any materials discussed in this article.},
  author       = {Dimitrijevic, Ivan and Malmsjö, Malin and Andersson, Christina and Rissler, Pehr and Edvinsson, Lars},
  issn         = {1549-4713},
  keyword      = {Temporal Arteries: metabolism,Type 2: metabolism,Angiotensin,Receptor,Type 1: metabolism,Vascular: metabolism,Smooth,Muscle,Endothelium,Giant Cell Arteritis: metabolism},
  language     = {eng},
  number       = {5},
  pages        = {990--996},
  publisher    = {Elsevier},
  series       = {Ophthalmology},
  title        = {Increased angiotensin II type 1 receptor expression in temporal arteries from patients with giant cell arteritis.},
  url          = {http://dx.doi.org/10.1016/j.ophtha.2008.12.021},
  volume       = {116},
  year         = {2009},
}