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Immobilized metal affinity chromatography in open-loop simulated moving bed technology: purification of a heat stable histidine tagged beta-glucosidase.

Sahoo, Deepti LU ; Andersson, Jonatan LU and Mattiasson, Bo LU (2009) In Journal of Chromatography. B 877(16-17). p.1651-1656
Abstract
Open-loop simulated moving bed (SMB) has been used for immobilized metal affinity chromatographic (IMAC) purification of his-tagged beta-glucosidase expressed in E. coli. A simplified approach based on an optimized single column protocol is used to design the open-loop SMB. A set of columns in the SMB represent one step in the chromatographic cycle i.e. there will be one set each of columns for load, wash, elution etc within the SMB. Only the wash and elution are operated with columns in sequence. The beta-glucosidase was purified to almost single band purity with a purification factor of 15 and a recovery of 91%. SMB-performance showed reduced buffer consumption, higher purification fold, a better yield and higher productivity.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
beta-Glucosidase: chemistry, Thermotoga neapolitana: enzymology, Recombinant Fusion Proteins: metabolism, beta-Glucosidase: genetics, beta-Glucosidase: metabolism, Recombinant Fusion Proteins: isolation & purification, Recombinant Fusion Proteins: genetics, Recombinant Fusion Proteins: chemistry, Oligopeptides: metabolism, Oligopeptides: isolation & purification, Oligopeptides: genetics, Oligopeptides: chemistry, Metals: chemistry, Histidine: metabolism, Histidine: isolation & purification, Histidine: genetics, Histidine: chemistry, Escherichia coli: metabolism, Affinity: methods, Chromatography, Escherichia coli: genetics, beta-Glucosidase: isolation & purification
in
Journal of Chromatography. B
volume
877
issue
16-17
pages
1651 - 1656
publisher
Elsevier
external identifiers
  • wos:000266687600006
  • pmid:19403343
  • scopus:65549115827
ISSN
1873-376X
DOI
10.1016/j.jchromb.2009.04.005
language
English
LU publication?
yes
id
d210cb1f-44f7-42f1-8bdc-62626b013048 (old id 1412849)
date added to LUP
2009-06-11 12:32:27
date last changed
2017-01-01 04:40:42
@article{d210cb1f-44f7-42f1-8bdc-62626b013048,
  abstract     = {Open-loop simulated moving bed (SMB) has been used for immobilized metal affinity chromatographic (IMAC) purification of his-tagged beta-glucosidase expressed in E. coli. A simplified approach based on an optimized single column protocol is used to design the open-loop SMB. A set of columns in the SMB represent one step in the chromatographic cycle i.e. there will be one set each of columns for load, wash, elution etc within the SMB. Only the wash and elution are operated with columns in sequence. The beta-glucosidase was purified to almost single band purity with a purification factor of 15 and a recovery of 91%. SMB-performance showed reduced buffer consumption, higher purification fold, a better yield and higher productivity.},
  author       = {Sahoo, Deepti and Andersson, Jonatan and Mattiasson, Bo},
  issn         = {1873-376X},
  keyword      = {beta-Glucosidase: chemistry,Thermotoga neapolitana: enzymology,Recombinant Fusion Proteins: metabolism,beta-Glucosidase: genetics,beta-Glucosidase: metabolism,Recombinant Fusion Proteins: isolation & purification,Recombinant Fusion Proteins: genetics,Recombinant Fusion Proteins: chemistry,Oligopeptides: metabolism,Oligopeptides: isolation & purification,Oligopeptides: genetics,Oligopeptides: chemistry,Metals: chemistry,Histidine: metabolism,Histidine: isolation & purification,Histidine: genetics,Histidine: chemistry,Escherichia coli: metabolism,Affinity: methods,Chromatography,Escherichia coli: genetics,beta-Glucosidase: isolation & purification},
  language     = {eng},
  number       = {16-17},
  pages        = {1651--1656},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography. B},
  title        = {Immobilized metal affinity chromatography in open-loop simulated moving bed technology: purification of a heat stable histidine tagged beta-glucosidase.},
  url          = {http://dx.doi.org/10.1016/j.jchromb.2009.04.005},
  volume       = {877},
  year         = {2009},
}