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The Native Reaction Centre of Photosystem II: A New Paradigm for P680

Hughes, Joseph L; Prince, Barry J; Peterson Årsköld, Sindra LU ; Smith, Paul J; Pace, Ron J; Riesen, Hans and Krausz, Elmars (2004) In Australian Journal of Chemistry 57(12). p.1179-1183
Abstract
Low-temperature spectra of fully active (oxygen-evolving) Photosystem II (PSII) cores prepared from spinach exhibit well developed structure. Spectra of isolated sub-fragments of PSII cores establish that the native reaction centre is better structured and red-shifted compared to the isolated reaction centre. Laser illumination of PSII cores leads to efficient and deep spectral hole-burning. Measurements of homogeneous hole-widths establish excited-state lifetimes in the 40–300 ps range. The high hole-burning efficiency is attributed to charge separation of P680 in native PSII that follows reaction-centre excitation via ‘slow transfer’ states in the inner light-harvesting assemblies CP43 and CP47. The ‘slow transfer’ state in CP47 and that... (More)
Low-temperature spectra of fully active (oxygen-evolving) Photosystem II (PSII) cores prepared from spinach exhibit well developed structure. Spectra of isolated sub-fragments of PSII cores establish that the native reaction centre is better structured and red-shifted compared to the isolated reaction centre. Laser illumination of PSII cores leads to efficient and deep spectral hole-burning. Measurements of homogeneous hole-widths establish excited-state lifetimes in the 40–300 ps range. The high hole-burning efficiency is attributed to charge separation of P680 in native PSII that follows reaction-centre excitation via ‘slow transfer’ states in the inner light-harvesting assemblies CP43 and CP47. The ‘slow transfer’ state in CP47 and that in CP43 can be distinguished in the hole-burning action spectrum and high-resolution hole-burning spectra. An important observation is that 685–700 nm illumination gives rise to efficient P680 charge separation, as established by QA− formation. This leads to a new paradigm for P680. The charge-separating state has surprisingly weak absorption and extends to 700 nm. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Australian Journal of Chemistry
volume
57
issue
12
pages
1179 - 1183
publisher
CSIRO Publishing
external identifiers
  • wos:000225599500014
  • scopus:11144253679
ISSN
0004-9425
DOI
10.1071/CH04140
language
English
LU publication?
yes
id
21e41b01-f55f-4531-8422-249447de1101 (old id 141363)
date added to LUP
2007-07-05 14:34:29
date last changed
2017-01-01 06:49:50
@article{21e41b01-f55f-4531-8422-249447de1101,
  abstract     = {Low-temperature spectra of fully active (oxygen-evolving) Photosystem II (PSII) cores prepared from spinach exhibit well developed structure. Spectra of isolated sub-fragments of PSII cores establish that the native reaction centre is better structured and red-shifted compared to the isolated reaction centre. Laser illumination of PSII cores leads to efficient and deep spectral hole-burning. Measurements of homogeneous hole-widths establish excited-state lifetimes in the 40–300 ps range. The high hole-burning efficiency is attributed to charge separation of P680 in native PSII that follows reaction-centre excitation via ‘slow transfer’ states in the inner light-harvesting assemblies CP43 and CP47. The ‘slow transfer’ state in CP47 and that in CP43 can be distinguished in the hole-burning action spectrum and high-resolution hole-burning spectra. An important observation is that 685–700 nm illumination gives rise to efficient P680 charge separation, as established by QA− formation. This leads to a new paradigm for P680. The charge-separating state has surprisingly weak absorption and extends to 700 nm.},
  author       = {Hughes, Joseph L and Prince, Barry J and Peterson Årsköld, Sindra and Smith, Paul J and Pace, Ron J and Riesen, Hans and Krausz, Elmars},
  issn         = {0004-9425},
  language     = {eng},
  number       = {12},
  pages        = {1179--1183},
  publisher    = {CSIRO Publishing},
  series       = {Australian Journal of Chemistry},
  title        = {The Native Reaction Centre of Photosystem II: A New Paradigm for P680},
  url          = {http://dx.doi.org/10.1071/CH04140},
  volume       = {57},
  year         = {2004},
}