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Arabidopsis Plasma Membrane Proteomics Identifies Components of Transport, Signal Transduction and Membrane Trafficking

Alexandersson, Erik LU ; Saalbach, Gerhard; Larsson, Christer LU and Kjellbom, Per LU (2004) In Plant and Cell Physiology 45(11). p.1543-1556
Abstract
In order to identify integral proteins and peripheral proteins associated with the plasma membrane, highly purified Arabidopsis plasma membranes from green tissue (leaves and petioles) were analyzed by mass spectrometry. Plasma membranes were isolated by aqueous two-phase partitioning, which yields plasma membrane vesicles with a cytoplasmic-side-in orientation and with a purity of 95%. These vesicles were turned inside-out by treatment with Brij 58 to remove soluble contaminating proteins enclosed in the vesicles and to remove loosely bound contaminating proteins. In total, 238 putative plasma membrane proteins were identified, of which 114 are predicted to have transmembrane domains or to be glycosyl phosphatidylinositol anchored. About... (More)
In order to identify integral proteins and peripheral proteins associated with the plasma membrane, highly purified Arabidopsis plasma membranes from green tissue (leaves and petioles) were analyzed by mass spectrometry. Plasma membranes were isolated by aqueous two-phase partitioning, which yields plasma membrane vesicles with a cytoplasmic-side-in orientation and with a purity of 95%. These vesicles were turned inside-out by treatment with Brij 58 to remove soluble contaminating proteins enclosed in the vesicles and to remove loosely bound contaminating proteins. In total, 238 putative plasma membrane proteins were identified, of which 114 are predicted to have transmembrane domains or to be glycosyl phosphatidylinositol anchored. About two-thirds of the identified integral proteins have not previously been shown to be plasma membrane proteins. Of the 238 identified proteins, 76% could be classified according to function. Major classes are proteins involved in transport (17%), signal transduction (16%), membrane trafficking (9%) and stress responses (9%). Almost a quarter of the proteins identified in the present study are functionally unclassified and more than half of these are predicted to be integral. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Plant and Cell Physiology
volume
45
issue
11
pages
1543 - 1556
publisher
Oxford University Press
external identifiers
  • pmid:15574830
  • wos:000225467800001
  • scopus:11144273935
ISSN
1471-9053
DOI
10.1093/pcp/pch209
language
English
LU publication?
yes
id
9694e30b-5d97-4c50-9196-049f3ffd4705 (old id 141372)
date added to LUP
2007-06-26 11:07:56
date last changed
2017-08-13 03:30:13
@article{9694e30b-5d97-4c50-9196-049f3ffd4705,
  abstract     = {In order to identify integral proteins and peripheral proteins associated with the plasma membrane, highly purified Arabidopsis plasma membranes from green tissue (leaves and petioles) were analyzed by mass spectrometry. Plasma membranes were isolated by aqueous two-phase partitioning, which yields plasma membrane vesicles with a cytoplasmic-side-in orientation and with a purity of 95%. These vesicles were turned inside-out by treatment with Brij 58 to remove soluble contaminating proteins enclosed in the vesicles and to remove loosely bound contaminating proteins. In total, 238 putative plasma membrane proteins were identified, of which 114 are predicted to have transmembrane domains or to be glycosyl phosphatidylinositol anchored. About two-thirds of the identified integral proteins have not previously been shown to be plasma membrane proteins. Of the 238 identified proteins, 76% could be classified according to function. Major classes are proteins involved in transport (17%), signal transduction (16%), membrane trafficking (9%) and stress responses (9%). Almost a quarter of the proteins identified in the present study are functionally unclassified and more than half of these are predicted to be integral.},
  author       = {Alexandersson, Erik and Saalbach, Gerhard and Larsson, Christer and Kjellbom, Per},
  issn         = {1471-9053},
  language     = {eng},
  number       = {11},
  pages        = {1543--1556},
  publisher    = {Oxford University Press},
  series       = {Plant and Cell Physiology},
  title        = {Arabidopsis Plasma Membrane Proteomics Identifies Components of Transport, Signal Transduction and Membrane Trafficking},
  url          = {http://dx.doi.org/10.1093/pcp/pch209},
  volume       = {45},
  year         = {2004},
}