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Continuous measurements of a binding reaction using a capacitive biosensor

Hedström, Martin LU ; Galaev, Igor LU and Mattiasson, Bo LU (2005) In Biosensors & Bioelectronics 21(1). p.41-48
Abstract
A capacitive biosensor with polyclonal antibodies raised against human serum albumin (HSA) immobilized on a gold transducer has been developed for continuous measurement of HSA in the μM-range. A mathematical model has been refined to describe integral HSA-binding curves assuming that (i) binding is essentially irreversible under the conditions used, (ii) the signal is scaled as the number of non-occupied binding sites and (iii) the rate of disappearance of available binding sites is scaled as the number of available binding sites and analyte concentration in solution. Deconvolution of the curves using the mathematical model indicates clearly that it is possible to retrieve concentration profiles (isocratic, linearly or exponentially... (More)
A capacitive biosensor with polyclonal antibodies raised against human serum albumin (HSA) immobilized on a gold transducer has been developed for continuous measurement of HSA in the μM-range. A mathematical model has been refined to describe integral HSA-binding curves assuming that (i) binding is essentially irreversible under the conditions used, (ii) the signal is scaled as the number of non-occupied binding sites and (iii) the rate of disappearance of available binding sites is scaled as the number of available binding sites and analyte concentration in solution. Deconvolution of the curves using the mathematical model indicates clearly that it is possible to retrieve concentration profiles (isocratic, linearly or exponentially increasing gradients) of the analyte in the continuous sample flow from the normalized integral binding (NIB) curves. The data presented constitutes the theoretical background and the first step towards the development of an analytical system allowing on-line detection of the concentration profile of the analyte from NIB-curves. Since the system can be used for extended time periods between regeneration steps, a low frequency of regeneration steps can be expected. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biosensors & Bioelectronics
volume
21
issue
1
pages
41 - 48
publisher
Elsevier
external identifiers
  • wos:000232268900004
  • scopus:20444480914
ISSN
1873-4235
DOI
10.1016/j.bios.2004.10.014
language
English
LU publication?
yes
id
de9a4166-dcf8-4f63-96eb-ed89e7aa5166 (old id 141637)
date added to LUP
2007-07-02 11:31:53
date last changed
2017-03-05 04:11:50
@article{de9a4166-dcf8-4f63-96eb-ed89e7aa5166,
  abstract     = {A capacitive biosensor with polyclonal antibodies raised against human serum albumin (HSA) immobilized on a gold transducer has been developed for continuous measurement of HSA in the μM-range. A mathematical model has been refined to describe integral HSA-binding curves assuming that (i) binding is essentially irreversible under the conditions used, (ii) the signal is scaled as the number of non-occupied binding sites and (iii) the rate of disappearance of available binding sites is scaled as the number of available binding sites and analyte concentration in solution. Deconvolution of the curves using the mathematical model indicates clearly that it is possible to retrieve concentration profiles (isocratic, linearly or exponentially increasing gradients) of the analyte in the continuous sample flow from the normalized integral binding (NIB) curves. The data presented constitutes the theoretical background and the first step towards the development of an analytical system allowing on-line detection of the concentration profile of the analyte from NIB-curves. Since the system can be used for extended time periods between regeneration steps, a low frequency of regeneration steps can be expected.},
  author       = {Hedström, Martin and Galaev, Igor and Mattiasson, Bo},
  issn         = {1873-4235},
  language     = {eng},
  number       = {1},
  pages        = {41--48},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {Continuous measurements of a binding reaction using a capacitive biosensor},
  url          = {http://dx.doi.org/10.1016/j.bios.2004.10.014},
  volume       = {21},
  year         = {2005},
}