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The human SIN3B corepressor forms a nucleolar complex with leukemia-associated ETO homologues

Dhanda, R. S.; Rondin Lindberg, Sofia LU and Olsson, Inge LU (2008) In BMC Molecular Biology 9. p.1-17
Abstract
Background: SIN3 ( SWI-Independent) is part of a transcriptional deacetylase complex, which generally mediates the formation of repressive chromatin. The purpose of this work was to study possible interactions between corepressors human SIN3B (hSIN3B) and the ETO homologues ETO ( eight twenty-one), MTG16 ( myeloid-transforming gene 16) and MTGR1 ( MTG-related protein 1). In addition, the subnuclear localization of the hSIN3B and the ETO homologues was also examined. Results: A ubiquitous expression of hSIN3B was observed in adult and fetal tissues. Results with both ectopically expressed proteins in COS-7 cells and endogeneous proteins in the K562 human erytholeukemia cell line demonstrated interactions between hSIN3B and ETO or MTG16 but... (More)
Background: SIN3 ( SWI-Independent) is part of a transcriptional deacetylase complex, which generally mediates the formation of repressive chromatin. The purpose of this work was to study possible interactions between corepressors human SIN3B (hSIN3B) and the ETO homologues ETO ( eight twenty-one), MTG16 ( myeloid-transforming gene 16) and MTGR1 ( MTG-related protein 1). In addition, the subnuclear localization of the hSIN3B and the ETO homologues was also examined. Results: A ubiquitous expression of hSIN3B was observed in adult and fetal tissues. Results with both ectopically expressed proteins in COS-7 cells and endogeneous proteins in the K562 human erytholeukemia cell line demonstrated interactions between hSIN3B and ETO or MTG16 but not MTGR1. Furthermore, nuclear extract of primary placental cells showed complexes between hSIN3B and ETO. The interaction between hSIN3B and ETO required an intact amino-terminus of ETO and the NHR2 domain. A nucleolar localization of hSIN3B and all the ETO homologues was demonstrated upon overexpression in COS-7 cells, and confirmed for the endogeneously expressed proteins in K562 cells. However, hSIN3B did not colocalize or interact with the leukemia-associated AML1 -ETO. Conclusion: Our data from protein-protein interactions and immunolocalization experiments support that hSIN3B is a potential member of a corepressor complex involving selective ETO homologues. (Less)
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published
subject
keywords
N-COR, HISTONE DEACETYLASE, FUSION PROTEIN, LEUKEMIA, REPRESSES, ACUTE MYELOID-LEUKEMIA, POLYMERASE-I TRANSCRIPTION, ACUTE MYELOGENOUS, TRANSCRIPTION, GENE-EXPRESSION, T(8-21), T(8/21)
in
BMC Molecular Biology
volume
9
pages
1 - 17
publisher
BioMed Central
external identifiers
  • wos:000253967600001
  • scopus:41449095241
ISSN
1471-2199
DOI
10.1186/1471-2199-9-8
language
English
LU publication?
yes
id
77c67321-5f9f-4a3b-9af1-91eb86a14960 (old id 1416451)
date added to LUP
2009-06-16 14:46:04
date last changed
2017-01-01 05:26:38
@article{77c67321-5f9f-4a3b-9af1-91eb86a14960,
  abstract     = {Background: SIN3 ( SWI-Independent) is part of a transcriptional deacetylase complex, which generally mediates the formation of repressive chromatin. The purpose of this work was to study possible interactions between corepressors human SIN3B (hSIN3B) and the ETO homologues ETO ( eight twenty-one), MTG16 ( myeloid-transforming gene 16) and MTGR1 ( MTG-related protein 1). In addition, the subnuclear localization of the hSIN3B and the ETO homologues was also examined. Results: A ubiquitous expression of hSIN3B was observed in adult and fetal tissues. Results with both ectopically expressed proteins in COS-7 cells and endogeneous proteins in the K562 human erytholeukemia cell line demonstrated interactions between hSIN3B and ETO or MTG16 but not MTGR1. Furthermore, nuclear extract of primary placental cells showed complexes between hSIN3B and ETO. The interaction between hSIN3B and ETO required an intact amino-terminus of ETO and the NHR2 domain. A nucleolar localization of hSIN3B and all the ETO homologues was demonstrated upon overexpression in COS-7 cells, and confirmed for the endogeneously expressed proteins in K562 cells. However, hSIN3B did not colocalize or interact with the leukemia-associated AML1 -ETO. Conclusion: Our data from protein-protein interactions and immunolocalization experiments support that hSIN3B is a potential member of a corepressor complex involving selective ETO homologues.},
  author       = {Dhanda, R. S. and Rondin Lindberg, Sofia and Olsson, Inge},
  issn         = {1471-2199},
  keyword      = {N-COR,HISTONE DEACETYLASE,FUSION PROTEIN,LEUKEMIA,REPRESSES,ACUTE MYELOID-LEUKEMIA,POLYMERASE-I TRANSCRIPTION,ACUTE MYELOGENOUS,TRANSCRIPTION,GENE-EXPRESSION,T(8-21),T(8/21)},
  language     = {eng},
  pages        = {1--17},
  publisher    = {BioMed Central},
  series       = {BMC Molecular Biology},
  title        = {The human SIN3B corepressor forms a nucleolar complex with leukemia-associated ETO homologues},
  url          = {http://dx.doi.org/10.1186/1471-2199-9-8},
  volume       = {9},
  year         = {2008},
}