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Continuous acetonitrile degradation in a packed-bed bioreactor

Manolov, Taras LU ; Håkansson, Kristina LU and Guieysse, Benoit LU (2005) In Applied Microbiology and Biotechnology 66(5). p.567-574
Abstract
A 20-l packed-bed reactor filled with foamed glass beads was tested for the treatment of acetonitrile HPLC wastes. Aeration was provided by recirculating a portion of the reactor liquid phase through an aeration tank, where the dissolved oxygen concentration was kept at 6 mg/l. At a feeding rate of 0.77 g acetonitrile l–1 reactor day–1, 99% of the acetonitrile was removed; and 86% of the nitrogen present in acetonitrile was released as NH3, confirming that acetonitrile volatilization was not significant. Increasing the acetonitrile loading resulted in lower removal efficiencies, but a maximum removal capacity of 1.0 g acetonitrile l–1 reactor day–1 was achieved at a feeding rate of 1.6 g acetonitrile l–1 reactor day–1. The removal capacity... (More)
A 20-l packed-bed reactor filled with foamed glass beads was tested for the treatment of acetonitrile HPLC wastes. Aeration was provided by recirculating a portion of the reactor liquid phase through an aeration tank, where the dissolved oxygen concentration was kept at 6 mg/l. At a feeding rate of 0.77 g acetonitrile l–1 reactor day–1, 99% of the acetonitrile was removed; and 86% of the nitrogen present in acetonitrile was released as NH3, confirming that acetonitrile volatilization was not significant. Increasing the acetonitrile loading resulted in lower removal efficiencies, but a maximum removal capacity of 1.0 g acetonitrile l–1 reactor day–1 was achieved at a feeding rate of 1.6 g acetonitrile l–1 reactor day–1. The removal capacity of the system was well correlated with the oxygenation capacity, showing that acetonitrile removal was likely to be limited by oxygen supply. Microbial characterization of the biofilm resulted in the isolation of a Comamonas sp. able to mineralize acetonitrile as sole carbon, nitrogen and energy source. This organism was closely related to C. testosteroni (91.2%) and might represent a new species in the Comamonas genus. This study confirms the potential of packed-bed reactors for the treatment of a concentrated mixture of volatile pollutants. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied Microbiology and Biotechnology
volume
66
issue
5
pages
567 - 574
publisher
Springer
external identifiers
  • pmid:15630519
  • wos:000226104200013
  • scopus:12544251090
ISSN
1432-0614
DOI
10.1007/s00253-004-1744-x
language
English
LU publication?
yes
id
ec805daa-b499-4499-b212-123836468ff0 (old id 141655)
date added to LUP
2007-07-03 09:05:09
date last changed
2017-01-01 06:55:12
@article{ec805daa-b499-4499-b212-123836468ff0,
  abstract     = {A 20-l packed-bed reactor filled with foamed glass beads was tested for the treatment of acetonitrile HPLC wastes. Aeration was provided by recirculating a portion of the reactor liquid phase through an aeration tank, where the dissolved oxygen concentration was kept at 6 mg/l. At a feeding rate of 0.77 g acetonitrile l–1 reactor day–1, 99% of the acetonitrile was removed; and 86% of the nitrogen present in acetonitrile was released as NH3, confirming that acetonitrile volatilization was not significant. Increasing the acetonitrile loading resulted in lower removal efficiencies, but a maximum removal capacity of 1.0 g acetonitrile l–1 reactor day–1 was achieved at a feeding rate of 1.6 g acetonitrile l–1 reactor day–1. The removal capacity of the system was well correlated with the oxygenation capacity, showing that acetonitrile removal was likely to be limited by oxygen supply. Microbial characterization of the biofilm resulted in the isolation of a Comamonas sp. able to mineralize acetonitrile as sole carbon, nitrogen and energy source. This organism was closely related to C. testosteroni (91.2%) and might represent a new species in the Comamonas genus. This study confirms the potential of packed-bed reactors for the treatment of a concentrated mixture of volatile pollutants.},
  author       = {Manolov, Taras and Håkansson, Kristina and Guieysse, Benoit},
  issn         = {1432-0614},
  language     = {eng},
  number       = {5},
  pages        = {567--574},
  publisher    = {Springer},
  series       = {Applied Microbiology and Biotechnology},
  title        = {Continuous acetonitrile degradation in a packed-bed bioreactor},
  url          = {http://dx.doi.org/10.1007/s00253-004-1744-x},
  volume       = {66},
  year         = {2005},
}