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Phosphatidylethanol as a sensitive and specific biomarker-comparison with gamma-glutamyl transpeptidase, mean corpuscular volume and carbohydrate-deficient transferrin

Hartmann, S.; Aradottir, Steina LU ; Graf, M.; Wiesbeck, G.; Lesch, O.; Ramskogler, K.; Wolfersdorf, M.; Alling, Christer LU and Wurst, F. M. (2007) In Addiction Biology 12(1). p.81-84
Abstract
Phosphatidylethanol (PEth), a direct ethanol metabolite, is detectable in blood for more than 2 weeks after sustained ethanol intake. Our aim was to assess the usefulness of PEth [comparing sensitivity, specificity and the area under the curve (AUC)] as compared with carbohydrate-deficient transferrin (CDT), gamma-glutamyl transpeptidase (GGT) and mean corpuscular volume (MCV), calculating the results from sober patients against those from alcohol-dependent patients during withdrawal. Fifty-six alcohol-dependent patients (ICD-10 F 10.25) in detoxification, age 43 years, GGT 81 U/l, MCV 96.4 fl, %CDT 4.2, 1400 g ethanol intake in the last 7 days (median), were included in the study. Over the time of 1 year, 52 samples from 35 sober forensic... (More)
Phosphatidylethanol (PEth), a direct ethanol metabolite, is detectable in blood for more than 2 weeks after sustained ethanol intake. Our aim was to assess the usefulness of PEth [comparing sensitivity, specificity and the area under the curve (AUC)] as compared with carbohydrate-deficient transferrin (CDT), gamma-glutamyl transpeptidase (GGT) and mean corpuscular volume (MCV), calculating the results from sober patients against those from alcohol-dependent patients during withdrawal. Fifty-six alcohol-dependent patients (ICD-10 F 10.25) in detoxification, age 43 years, GGT 81 U/l, MCV 96.4 fl, %CDT 4.2, 1400 g ethanol intake in the last 7 days (median), were included in the study. Over the time of 1 year, 52 samples from 35 sober forensic psychiatric addicted in-patients [age 34 years, GGT 16 U/l, MCV 91 fl, CDT 0.5 (median)] in a closed ward were drawn and used for comparison . PEth was measured in heparinized whole blood with a high-performance liquid chromatography method. GGT, MCV and %CDT were measured using routine methods. A receiver operating characteristic curve analysis was carried out, with 'current drinking status' (sober/drinking) as the state variable and PEth, MCV, GGT and CDT as test variables. The resulting AUC was 0.974 (P < 0.0001, confidence interval 0.932-1.016) for PEth. At a cut-off of 0.36 mu mol/l, the sensitivity was 94.5% and specificity 100%. The AUC for CDT, GGT and MCV were 0.931, 0.894 and 0.883, respectively. A significant Spearman's rank correlation was found between PEth and GGT (r = 0.739), CDT (r = 0.643), MVC (r = 0.639) and grams of ethanol consumed in the last 7 days (r = 0.802). Our data suggest that PEth has potential to be a sensitive and specific biomarker, having been found in previous studies to indicate longer lasting intake of higher amounts of alcohol. (Less)
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published
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keywords
ALCOHOL-USE, mean corpuscular, MALES, STATE, BRAIN, gamma-glutamyl transpeptidase, specificity, phosphatidylethanol, sensitivity, volume, PHOSPHOLIPASE-D, RAT ORGANS, BLOOD, MARKERS, ETHANOL TREATMENT, harmful ethanol intake
in
Addiction Biology
volume
12
issue
1
pages
81 - 84
publisher
Wiley-Blackwell
external identifiers
  • wos:000244417000010
  • scopus:33947182580
ISSN
1369-1600
DOI
10.1111/j.1369-1600.2006.00040.x
language
English
LU publication?
yes
id
c077ced3-e884-40cc-82d3-5410df6399d5 (old id 1416871)
date added to LUP
2009-06-16 11:14:22
date last changed
2017-10-22 03:42:40
@article{c077ced3-e884-40cc-82d3-5410df6399d5,
  abstract     = {Phosphatidylethanol (PEth), a direct ethanol metabolite, is detectable in blood for more than 2 weeks after sustained ethanol intake. Our aim was to assess the usefulness of PEth [comparing sensitivity, specificity and the area under the curve (AUC)] as compared with carbohydrate-deficient transferrin (CDT), gamma-glutamyl transpeptidase (GGT) and mean corpuscular volume (MCV), calculating the results from sober patients against those from alcohol-dependent patients during withdrawal. Fifty-six alcohol-dependent patients (ICD-10 F 10.25) in detoxification, age 43 years, GGT 81 U/l, MCV 96.4 fl, %CDT 4.2, 1400 g ethanol intake in the last 7 days (median), were included in the study. Over the time of 1 year, 52 samples from 35 sober forensic psychiatric addicted in-patients [age 34 years, GGT 16 U/l, MCV 91 fl, CDT 0.5 (median)] in a closed ward were drawn and used for comparison . PEth was measured in heparinized whole blood with a high-performance liquid chromatography method. GGT, MCV and %CDT were measured using routine methods. A receiver operating characteristic curve analysis was carried out, with 'current drinking status' (sober/drinking) as the state variable and PEth, MCV, GGT and CDT as test variables. The resulting AUC was 0.974 (P &lt; 0.0001, confidence interval 0.932-1.016) for PEth. At a cut-off of 0.36 mu mol/l, the sensitivity was 94.5% and specificity 100%. The AUC for CDT, GGT and MCV were 0.931, 0.894 and 0.883, respectively. A significant Spearman's rank correlation was found between PEth and GGT (r = 0.739), CDT (r = 0.643), MVC (r = 0.639) and grams of ethanol consumed in the last 7 days (r = 0.802). Our data suggest that PEth has potential to be a sensitive and specific biomarker, having been found in previous studies to indicate longer lasting intake of higher amounts of alcohol.},
  author       = {Hartmann, S. and Aradottir, Steina and Graf, M. and Wiesbeck, G. and Lesch, O. and Ramskogler, K. and Wolfersdorf, M. and Alling, Christer and Wurst, F. M.},
  issn         = {1369-1600},
  keyword      = {ALCOHOL-USE,mean corpuscular,MALES,STATE,BRAIN,gamma-glutamyl transpeptidase,specificity,phosphatidylethanol,sensitivity,volume,PHOSPHOLIPASE-D,RAT ORGANS,BLOOD,MARKERS,ETHANOL TREATMENT,harmful ethanol intake},
  language     = {eng},
  number       = {1},
  pages        = {81--84},
  publisher    = {Wiley-Blackwell},
  series       = {Addiction Biology},
  title        = {Phosphatidylethanol as a sensitive and specific biomarker-comparison with gamma-glutamyl transpeptidase, mean corpuscular volume and carbohydrate-deficient transferrin},
  url          = {http://dx.doi.org/10.1111/j.1369-1600.2006.00040.x},
  volume       = {12},
  year         = {2007},
}