Phosphatidylethanol as a sensitive and specific biomarker-comparison with gamma-glutamyl transpeptidase, mean corpuscular volume and carbohydrate-deficient transferrin
(2007) In Addiction Biology 12(1). p.81-84- Abstract
- Phosphatidylethanol (PEth), a direct ethanol metabolite, is detectable in blood for more than 2 weeks after sustained ethanol intake. Our aim was to assess the usefulness of PEth [comparing sensitivity, specificity and the area under the curve (AUC)] as compared with carbohydrate-deficient transferrin (CDT), gamma-glutamyl transpeptidase (GGT) and mean corpuscular volume (MCV), calculating the results from sober patients against those from alcohol-dependent patients during withdrawal. Fifty-six alcohol-dependent patients (ICD-10 F 10.25) in detoxification, age 43 years, GGT 81 U/l, MCV 96.4 fl, %CDT 4.2, 1400 g ethanol intake in the last 7 days (median), were included in the study. Over the time of 1 year, 52 samples from 35 sober forensic... (More)
- Phosphatidylethanol (PEth), a direct ethanol metabolite, is detectable in blood for more than 2 weeks after sustained ethanol intake. Our aim was to assess the usefulness of PEth [comparing sensitivity, specificity and the area under the curve (AUC)] as compared with carbohydrate-deficient transferrin (CDT), gamma-glutamyl transpeptidase (GGT) and mean corpuscular volume (MCV), calculating the results from sober patients against those from alcohol-dependent patients during withdrawal. Fifty-six alcohol-dependent patients (ICD-10 F 10.25) in detoxification, age 43 years, GGT 81 U/l, MCV 96.4 fl, %CDT 4.2, 1400 g ethanol intake in the last 7 days (median), were included in the study. Over the time of 1 year, 52 samples from 35 sober forensic psychiatric addicted in-patients [age 34 years, GGT 16 U/l, MCV 91 fl, CDT 0.5 (median)] in a closed ward were drawn and used for comparison . PEth was measured in heparinized whole blood with a high-performance liquid chromatography method. GGT, MCV and %CDT were measured using routine methods. A receiver operating characteristic curve analysis was carried out, with 'current drinking status' (sober/drinking) as the state variable and PEth, MCV, GGT and CDT as test variables. The resulting AUC was 0.974 (P < 0.0001, confidence interval 0.932-1.016) for PEth. At a cut-off of 0.36 mu mol/l, the sensitivity was 94.5% and specificity 100%. The AUC for CDT, GGT and MCV were 0.931, 0.894 and 0.883, respectively. A significant Spearman's rank correlation was found between PEth and GGT (r = 0.739), CDT (r = 0.643), MVC (r = 0.639) and grams of ethanol consumed in the last 7 days (r = 0.802). Our data suggest that PEth has potential to be a sensitive and specific biomarker, having been found in previous studies to indicate longer lasting intake of higher amounts of alcohol. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1416871
- author
- Hartmann, S. ; Aradottir, Steina LU ; Graf, M. ; Wiesbeck, G. ; Lesch, O. ; Ramskogler, K. ; Wolfersdorf, M. ; Alling, Christer LU and Wurst, F. M.
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- ALCOHOL-USE, mean corpuscular, MALES, STATE, BRAIN, gamma-glutamyl transpeptidase, specificity, phosphatidylethanol, sensitivity, volume, PHOSPHOLIPASE-D, RAT ORGANS, BLOOD, MARKERS, ETHANOL TREATMENT, harmful ethanol intake
- in
- Addiction Biology
- volume
- 12
- issue
- 1
- pages
- 81 - 84
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000244417000010
- scopus:33947182580
- pmid:17407500
- ISSN
- 1369-1600
- DOI
- 10.1111/j.1369-1600.2006.00040.x
- language
- English
- LU publication?
- yes
- id
- c077ced3-e884-40cc-82d3-5410df6399d5 (old id 1416871)
- date added to LUP
- 2016-04-01 11:58:47
- date last changed
- 2022-04-28 22:50:32
@article{c077ced3-e884-40cc-82d3-5410df6399d5, abstract = {{Phosphatidylethanol (PEth), a direct ethanol metabolite, is detectable in blood for more than 2 weeks after sustained ethanol intake. Our aim was to assess the usefulness of PEth [comparing sensitivity, specificity and the area under the curve (AUC)] as compared with carbohydrate-deficient transferrin (CDT), gamma-glutamyl transpeptidase (GGT) and mean corpuscular volume (MCV), calculating the results from sober patients against those from alcohol-dependent patients during withdrawal. Fifty-six alcohol-dependent patients (ICD-10 F 10.25) in detoxification, age 43 years, GGT 81 U/l, MCV 96.4 fl, %CDT 4.2, 1400 g ethanol intake in the last 7 days (median), were included in the study. Over the time of 1 year, 52 samples from 35 sober forensic psychiatric addicted in-patients [age 34 years, GGT 16 U/l, MCV 91 fl, CDT 0.5 (median)] in a closed ward were drawn and used for comparison . PEth was measured in heparinized whole blood with a high-performance liquid chromatography method. GGT, MCV and %CDT were measured using routine methods. A receiver operating characteristic curve analysis was carried out, with 'current drinking status' (sober/drinking) as the state variable and PEth, MCV, GGT and CDT as test variables. The resulting AUC was 0.974 (P < 0.0001, confidence interval 0.932-1.016) for PEth. At a cut-off of 0.36 mu mol/l, the sensitivity was 94.5% and specificity 100%. The AUC for CDT, GGT and MCV were 0.931, 0.894 and 0.883, respectively. A significant Spearman's rank correlation was found between PEth and GGT (r = 0.739), CDT (r = 0.643), MVC (r = 0.639) and grams of ethanol consumed in the last 7 days (r = 0.802). Our data suggest that PEth has potential to be a sensitive and specific biomarker, having been found in previous studies to indicate longer lasting intake of higher amounts of alcohol.}}, author = {{Hartmann, S. and Aradottir, Steina and Graf, M. and Wiesbeck, G. and Lesch, O. and Ramskogler, K. and Wolfersdorf, M. and Alling, Christer and Wurst, F. M.}}, issn = {{1369-1600}}, keywords = {{ALCOHOL-USE; mean corpuscular; MALES; STATE; BRAIN; gamma-glutamyl transpeptidase; specificity; phosphatidylethanol; sensitivity; volume; PHOSPHOLIPASE-D; RAT ORGANS; BLOOD; MARKERS; ETHANOL TREATMENT; harmful ethanol intake}}, language = {{eng}}, number = {{1}}, pages = {{81--84}}, publisher = {{Wiley-Blackwell}}, series = {{Addiction Biology}}, title = {{Phosphatidylethanol as a sensitive and specific biomarker-comparison with gamma-glutamyl transpeptidase, mean corpuscular volume and carbohydrate-deficient transferrin}}, url = {{http://dx.doi.org/10.1111/j.1369-1600.2006.00040.x}}, doi = {{10.1111/j.1369-1600.2006.00040.x}}, volume = {{12}}, year = {{2007}}, }