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Coupled diffusion and adsorption effects for multiple proteins in agarose gel

Gutenwik, Jan LU ; Nilsson, Bernt LU and Axelsson, Anders LU (2004) In AICHE Journal 50(12). p.3006-3018
Abstract
A mathematical model for the hindered diffusion and competitive adsorption of two proteins in an agarose gel has been developed. In a simulation program the model has been used to study the competing and displacement effects on a single bead for lysozyme and bovine serum albumin (BSA) bound to the ligand Cibacron Blue in agarose gel. The model takes into account hindered diffusion described by the Renkin model, competitive Langmuir adsorption kinetics, pore size distribution of the gel, and a shrinking effective pore radius attributed to molecule-to-ligand binding. The simulation model can easily explain displacement of BSA or lysozyme dependent on the binding capacity, kinetics, and diffusion. The influence of a bimodal pore size... (More)
A mathematical model for the hindered diffusion and competitive adsorption of two proteins in an agarose gel has been developed. In a simulation program the model has been used to study the competing and displacement effects on a single bead for lysozyme and bovine serum albumin (BSA) bound to the ligand Cibacron Blue in agarose gel. The model takes into account hindered diffusion described by the Renkin model, competitive Langmuir adsorption kinetics, pore size distribution of the gel, and a shrinking effective pore radius attributed to molecule-to-ligand binding. The simulation model can easily explain displacement of BSA or lysozyme dependent on the binding capacity, kinetics, and diffusion. The influence of a bimodal pore size distribution is demonstrated. It also provides insight into the phenomenon of static vs. dynamic binding capacity observed in experimental determinations of the isotherm. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
AICHE Journal
volume
50
issue
12
pages
3006 - 3018
publisher
Wiley-Blackwell
external identifiers
  • wos:000225445000003
  • scopus:10844275580
ISSN
1547-5905
DOI
10.1002/aic.10231
language
English
LU publication?
yes
id
1abb3af6-fabd-4472-b062-c512f6fd5db7 (old id 141700)
date added to LUP
2007-06-25 10:59:08
date last changed
2017-09-17 04:49:30
@article{1abb3af6-fabd-4472-b062-c512f6fd5db7,
  abstract     = {A mathematical model for the hindered diffusion and competitive adsorption of two proteins in an agarose gel has been developed. In a simulation program the model has been used to study the competing and displacement effects on a single bead for lysozyme and bovine serum albumin (BSA) bound to the ligand Cibacron Blue in agarose gel. The model takes into account hindered diffusion described by the Renkin model, competitive Langmuir adsorption kinetics, pore size distribution of the gel, and a shrinking effective pore radius attributed to molecule-to-ligand binding. The simulation model can easily explain displacement of BSA or lysozyme dependent on the binding capacity, kinetics, and diffusion. The influence of a bimodal pore size distribution is demonstrated. It also provides insight into the phenomenon of static vs. dynamic binding capacity observed in experimental determinations of the isotherm.},
  author       = {Gutenwik, Jan and Nilsson, Bernt and Axelsson, Anders},
  issn         = {1547-5905},
  language     = {eng},
  number       = {12},
  pages        = {3006--3018},
  publisher    = {Wiley-Blackwell},
  series       = {AICHE Journal},
  title        = {Coupled diffusion and adsorption effects for multiple proteins in agarose gel},
  url          = {http://dx.doi.org/10.1002/aic.10231},
  volume       = {50},
  year         = {2004},
}