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Characterization of lipopolysaccharides present in settled house dust

Park, J H; Szponar, Bogumila LU ; Larsson, Lennart LU ; Gold, D R and Milton, D K (2004) In Applied and Environmental Microbiology 70(1). p.262-267
Abstract
The 3-hydroxy fatty acids (3-OHFAs) in lipopolysaccharides (LPS) play an important role in determining endotoxin activity, and childhood exposure to endotoxin has recently been associated with reduced risk of atopic diseases. To characterize the 3-OHFAs in house dust (HD), we used gas chromatography-mass spectrometry to assay 190 HD samples. Dust from beds, bedroom floors, family rooms, and kitchen floors was collected as part of a birth cohort study of childhood asthma (study 1) and a longitudinal study of home allergen and endotoxin (study 2). We also measured endotoxin activity with a Limulus assay and computed specific activity (endotoxin activity per nanomole of LPS). Longer-chain (C16:0 and C18:0) 3-OHFAs were predominant in HD... (More)
The 3-hydroxy fatty acids (3-OHFAs) in lipopolysaccharides (LPS) play an important role in determining endotoxin activity, and childhood exposure to endotoxin has recently been associated with reduced risk of atopic diseases. To characterize the 3-OHFAs in house dust (HD), we used gas chromatography-mass spectrometry to assay 190 HD samples. Dust from beds, bedroom floors, family rooms, and kitchen floors was collected as part of a birth cohort study of childhood asthma (study 1) and a longitudinal study of home allergen and endotoxin (study 2). We also measured endotoxin activity with a Limulus assay and computed specific activity (endotoxin activity per nanomole of LPS). Longer-chain (C16:0 and C18:0) 3-OHFAs were predominant in HD compared with short-chain (C10:0, C12:0, and C14:0) acids. Endotoxin activity was positively correlated with short-chain 3-OHFAs in both studies. In study 2, 3-OH C16:0 was negatively correlated and 3-OH C18:0 was not correlated with endotoxin activity, consistent with previous findings that the Limulus assay responds preferentially to LPS containing short-chain 3-OHFAs. Kitchen dust contained the highest concentrations of 3-OH C10:0, the highest endotoxin activities, and the highest specific activities (P < 0.03). Bed dust contained the largest amounts of long-chain 3-OHFAs, the highest concentrations of LPS, and the lowest specific activities. Apartments had significantly different types of LPS (P = 0.03) compared with single-family homes in study 2. These data suggest that the Limulus assay may underestimate exposure to certain types of LPS. Because nontoxic LPS may have immune modulating effects, analysis of 3-OHFAs may be useful in epidemiologic studies. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied and Environmental Microbiology
volume
70
issue
1
pages
262 - 267
publisher
American Society for Microbiology
external identifiers
  • pmid:14711650
  • wos:000188115300032
  • scopus:0347761176
ISSN
0099-2240
DOI
10.1128/AEM.70.1.262-267.2004
language
English
LU publication?
yes
id
b2288a16-99b9-4595-8bca-410a9662bee1 (old id 141802)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=14711650&query_hl=59
date added to LUP
2007-07-04 10:36:08
date last changed
2017-01-01 05:08:29
@article{b2288a16-99b9-4595-8bca-410a9662bee1,
  abstract     = {The 3-hydroxy fatty acids (3-OHFAs) in lipopolysaccharides (LPS) play an important role in determining endotoxin activity, and childhood exposure to endotoxin has recently been associated with reduced risk of atopic diseases. To characterize the 3-OHFAs in house dust (HD), we used gas chromatography-mass spectrometry to assay 190 HD samples. Dust from beds, bedroom floors, family rooms, and kitchen floors was collected as part of a birth cohort study of childhood asthma (study 1) and a longitudinal study of home allergen and endotoxin (study 2). We also measured endotoxin activity with a Limulus assay and computed specific activity (endotoxin activity per nanomole of LPS). Longer-chain (C16:0 and C18:0) 3-OHFAs were predominant in HD compared with short-chain (C10:0, C12:0, and C14:0) acids. Endotoxin activity was positively correlated with short-chain 3-OHFAs in both studies. In study 2, 3-OH C16:0 was negatively correlated and 3-OH C18:0 was not correlated with endotoxin activity, consistent with previous findings that the Limulus assay responds preferentially to LPS containing short-chain 3-OHFAs. Kitchen dust contained the highest concentrations of 3-OH C10:0, the highest endotoxin activities, and the highest specific activities (P &lt; 0.03). Bed dust contained the largest amounts of long-chain 3-OHFAs, the highest concentrations of LPS, and the lowest specific activities. Apartments had significantly different types of LPS (P = 0.03) compared with single-family homes in study 2. These data suggest that the Limulus assay may underestimate exposure to certain types of LPS. Because nontoxic LPS may have immune modulating effects, analysis of 3-OHFAs may be useful in epidemiologic studies.},
  author       = {Park, J H and Szponar, Bogumila and Larsson, Lennart and Gold, D R and Milton, D K},
  issn         = {0099-2240},
  language     = {eng},
  number       = {1},
  pages        = {262--267},
  publisher    = {American Society for Microbiology},
  series       = {Applied and Environmental Microbiology},
  title        = {Characterization of lipopolysaccharides present in settled house dust},
  url          = {http://dx.doi.org/10.1128/AEM.70.1.262-267.2004},
  volume       = {70},
  year         = {2004},
}