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Isolation and characterization of two European strains of Ehrlichia phagocytophila of equine origin

Bjöersdorff, Anneli; Bagert, Bodil; Massung, Robert F.; Gusa, Asiya and Eliasson, Ingvar LU (2002) In Clinical and Diagnostic Laboratory Immunology 9(2). p.341-343
Abstract
We report the isolation and partial genetic characterization of two equine strains of granulocytic Ehrlichia of the genogroup Ehrlichia phagocytophila. Frozen whole-blood samples from two Swedish horses with laboratory-verified granulocytic ehrlichiosis were inoculated into HL-60 cell cultures. Granulocytic Ehrlichia was isolated and propagated from both horses. DNA extracts from the respective strains were amplified by PCR using primers directed towards the 16S rRNA gene, the groESL heat shock operon gene, and the ank gene. The amplified gene fragments were sequenced and compared to known sequences in the GenBank database. With respect to the 16S rRNA gene, the groESL gene, and the ank gene, the DNA sequences of the two equine Ehrlichia... (More)
We report the isolation and partial genetic characterization of two equine strains of granulocytic Ehrlichia of the genogroup Ehrlichia phagocytophila. Frozen whole-blood samples from two Swedish horses with laboratory-verified granulocytic ehrlichiosis were inoculated into HL-60 cell cultures. Granulocytic Ehrlichia was isolated and propagated from both horses. DNA extracts from the respective strains were amplified by PCR using primers directed towards the 16S rRNA gene, the groESL heat shock operon gene, and the ank gene. The amplified gene fragments were sequenced and compared to known sequences in the GenBank database. With respect to the 16S rRNA gene, the groESL gene, and the ank gene, the DNA sequences of the two equine Ehrlichia isolates were identical to sequences found in isolates from clinical cases of granulocytic ehrlichiosis in humans and domestic animals in Sweden. However, compared to amplified DNA from an American Ehrlichia strain of the E. phagocytophila genogroup, differences were found in the groESL gene and ank gene sequences. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Clinical and Diagnostic Laboratory Immunology
volume
9
issue
2
pages
341 - 343
publisher
American Society for Microbiology
external identifiers
  • wos:000174457600021
  • pmid:11874874
  • scopus:0036127802
ISSN
1071-412X
DOI
10.1128/CDLI.9.2.341-343.2002
language
English
LU publication?
yes
id
ae1a73fb-fff4-4baf-b574-db3787f14b65 (old id 141824)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11874874&query_hl=29
date added to LUP
2007-07-09 15:30:55
date last changed
2017-01-01 06:38:06
@article{ae1a73fb-fff4-4baf-b574-db3787f14b65,
  abstract     = {We report the isolation and partial genetic characterization of two equine strains of granulocytic Ehrlichia of the genogroup Ehrlichia phagocytophila. Frozen whole-blood samples from two Swedish horses with laboratory-verified granulocytic ehrlichiosis were inoculated into HL-60 cell cultures. Granulocytic Ehrlichia was isolated and propagated from both horses. DNA extracts from the respective strains were amplified by PCR using primers directed towards the 16S rRNA gene, the groESL heat shock operon gene, and the ank gene. The amplified gene fragments were sequenced and compared to known sequences in the GenBank database. With respect to the 16S rRNA gene, the groESL gene, and the ank gene, the DNA sequences of the two equine Ehrlichia isolates were identical to sequences found in isolates from clinical cases of granulocytic ehrlichiosis in humans and domestic animals in Sweden. However, compared to amplified DNA from an American Ehrlichia strain of the E. phagocytophila genogroup, differences were found in the groESL gene and ank gene sequences.},
  author       = {Bjöersdorff, Anneli and Bagert, Bodil and Massung, Robert F. and Gusa, Asiya and Eliasson, Ingvar},
  issn         = {1071-412X},
  language     = {eng},
  number       = {2},
  pages        = {341--343},
  publisher    = {American Society for Microbiology},
  series       = {Clinical and Diagnostic Laboratory Immunology},
  title        = {Isolation and characterization of two European strains of Ehrlichia phagocytophila of equine origin},
  url          = {http://dx.doi.org/10.1128/CDLI.9.2.341-343.2002},
  volume       = {9},
  year         = {2002},
}