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Direct colorimetric assay for rapid detection of rifampin-resistant Mycobacterium tuberculosis

Abate, Getahun; Aseffa, Abraham; Selassie, Alemayehu; Goshu, Solomon; Fekade, Bekele; WoldeMeskal, Dawit and Miörner, Håkan LU (2004) In Journal of Clinical Microbiology 42(2). p.871-871
Abstract
The colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was standardized for direct detection of rifampin-resistant Mycobacterium tuberculosis in sputum samples. The sensitivity and specificity of the direct MTT assay matched those of the standard indirect susceptibility assay on 7H10 medium, and interpretable results were obtained for 98.5% of the samples within 2 weeks. Traditional methods of in vitro drug susceptibility testing are time consuming and laborious. Susceptibility tests on clinical isolates require 6 to 9 weeks, and tests conducted directly on smear-positive samples take about 3 weeks (International Union Against Tuberculosis and Lung Disease, The public health service national tuberculosis... (More)
The colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was standardized for direct detection of rifampin-resistant Mycobacterium tuberculosis in sputum samples. The sensitivity and specificity of the direct MTT assay matched those of the standard indirect susceptibility assay on 7H10 medium, and interpretable results were obtained for 98.5% of the samples within 2 weeks. Traditional methods of in vitro drug susceptibility testing are time consuming and laborious. Susceptibility tests on clinical isolates require 6 to 9 weeks, and tests conducted directly on smear-positive samples take about 3 weeks (International Union Against Tuberculosis and Lung Disease, The public health service national tuberculosis reference laboratory and the national laboratory network. Minimum requirements, role and operation in a low-income country, Paris, France, 1998, and P. T. Kent and G. P. Kubica, Public health mycobacteriology. A guide for the level III laboratory, Centers for Disease Control and Prevention, Atlanta, Ga., 1985). More-rapid methods are available but are very expensive for routine use under program conditions in countries with high levels of tuberculosis endemicity. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Journal of Clinical Microbiology
volume
42
issue
2
pages
871 - 871
publisher
American Society for Microbiology
external identifiers
  • pmid:14766876
  • wos:000189379000071
  • scopus:1242291827
ISSN
1098-660X
DOI
10.1128/JCM.42.2.871-873.2004
language
English
LU publication?
yes
id
ec0a3db2-6375-4444-b969-fd4c516197f3 (old id 141894)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=14766876&query_hl=41
date added to LUP
2007-06-26 13:52:48
date last changed
2017-01-22 04:07:59
@article{ec0a3db2-6375-4444-b969-fd4c516197f3,
  abstract     = {The colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was standardized for direct detection of rifampin-resistant Mycobacterium tuberculosis in sputum samples. The sensitivity and specificity of the direct MTT assay matched those of the standard indirect susceptibility assay on 7H10 medium, and interpretable results were obtained for 98.5% of the samples within 2 weeks. Traditional methods of in vitro drug susceptibility testing are time consuming and laborious. Susceptibility tests on clinical isolates require 6 to 9 weeks, and tests conducted directly on smear-positive samples take about 3 weeks (International Union Against Tuberculosis and Lung Disease, The public health service national tuberculosis reference laboratory and the national laboratory network. Minimum requirements, role and operation in a low-income country, Paris, France, 1998, and P. T. Kent and G. P. Kubica, Public health mycobacteriology. A guide for the level III laboratory, Centers for Disease Control and Prevention, Atlanta, Ga., 1985). More-rapid methods are available but are very expensive for routine use under program conditions in countries with high levels of tuberculosis endemicity.},
  author       = {Abate, Getahun and Aseffa, Abraham and Selassie, Alemayehu and Goshu, Solomon and Fekade, Bekele and WoldeMeskal, Dawit and Miörner, Håkan},
  issn         = {1098-660X},
  language     = {eng},
  number       = {2},
  pages        = {871--871},
  publisher    = {American Society for Microbiology},
  series       = {Journal of Clinical Microbiology},
  title        = {Direct colorimetric assay for rapid detection of rifampin-resistant Mycobacterium tuberculosis},
  url          = {http://dx.doi.org/10.1128/JCM.42.2.871-873.2004},
  volume       = {42},
  year         = {2004},
}