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Inhibition of p300/CBP by early B-cell factor

Zhao, Fang; McCarrick-Walmsley, Ruth; Åkerblad, Peter; Sigvardsson, Mikael LU and Kadesch, Tom (2003) In Molecular and Cellular Biology 23(11). p.3837-3846
Abstract
Early B-cell factor (EBF) is a DNA binding protein required for early B-cell development. It activates transcription of several B-cell-specific genes, including the {lambda}5 gene, which encodes a protein necessary for signaling by the pre-B-cell receptor. In an effort to understand the mechanism by which EBF activates transcription, we examined its interaction with the coactivator protein p300/CBP. We found that two domains of EBF each bind the histone acetyltransferase (HAT)/CH3 domain of p300/CBP both in vitro and in vivo. Surprisingly, transcriptional activation by EBF was not sensitive to E1A, a potent p300/CBP inhibitor. In fact, overexpressed EBF mimicked E1A by severely repressing the activity of several other transcription... (More)
Early B-cell factor (EBF) is a DNA binding protein required for early B-cell development. It activates transcription of several B-cell-specific genes, including the {lambda}5 gene, which encodes a protein necessary for signaling by the pre-B-cell receptor. In an effort to understand the mechanism by which EBF activates transcription, we examined its interaction with the coactivator protein p300/CBP. We found that two domains of EBF each bind the histone acetyltransferase (HAT)/CH3 domain of p300/CBP both in vitro and in vivo. Surprisingly, transcriptional activation by EBF was not sensitive to E1A, a potent p300/CBP inhibitor. In fact, overexpressed EBF mimicked E1A by severely repressing the activity of several other transcription factors, including E47, a protein that acts cooperatively with EBF to promote transcription of the {lambda}5 gene. This broad inhibitory profile correlated with EBF's ability to repress the HAT activity of p300/CBP in vivo and in vitro. However, such a repressed complex is not likely to form at the {lambda}5 promoter in vivo since (i) EBF could not bind p300/CBP and DNA simultaneously and (ii) the cooperativity imparted by E47 was sensitive to E1A. Our data reveal an intriguing inhibitory property of EBF—a property shared only by E1A, Twist, Pu.1, and the Hox family of homeodomain proteins—and suggest that E47 and EBF play distinct roles during {lambda}5 promoter activation. (Less)
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author
organization
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Contribution to journal
publication status
published
subject
in
Molecular and Cellular Biology
volume
23
issue
11
pages
3837 - 3846
publisher
American Society for Microbiology
external identifiers
  • wos:000183031900011
  • pmid:12748286
  • scopus:0037570593
ISSN
0270-7306
DOI
language
English
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yes
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6c98d4a1-a57f-4913-b140-e3330eac3a2f (old id 141935)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12748286&query_hl=39
date added to LUP
2007-07-24 09:57:41
date last changed
2018-05-29 12:02:01
@article{6c98d4a1-a57f-4913-b140-e3330eac3a2f,
  abstract     = {Early B-cell factor (EBF) is a DNA binding protein required for early B-cell development. It activates transcription of several B-cell-specific genes, including the {lambda}5 gene, which encodes a protein necessary for signaling by the pre-B-cell receptor. In an effort to understand the mechanism by which EBF activates transcription, we examined its interaction with the coactivator protein p300/CBP. We found that two domains of EBF each bind the histone acetyltransferase (HAT)/CH3 domain of p300/CBP both in vitro and in vivo. Surprisingly, transcriptional activation by EBF was not sensitive to E1A, a potent p300/CBP inhibitor. In fact, overexpressed EBF mimicked E1A by severely repressing the activity of several other transcription factors, including E47, a protein that acts cooperatively with EBF to promote transcription of the {lambda}5 gene. This broad inhibitory profile correlated with EBF's ability to repress the HAT activity of p300/CBP in vivo and in vitro. However, such a repressed complex is not likely to form at the {lambda}5 promoter in vivo since (i) EBF could not bind p300/CBP and DNA simultaneously and (ii) the cooperativity imparted by E47 was sensitive to E1A. Our data reveal an intriguing inhibitory property of EBF—a property shared only by E1A, Twist, Pu.1, and the Hox family of homeodomain proteins—and suggest that E47 and EBF play distinct roles during {lambda}5 promoter activation.},
  author       = {Zhao, Fang and McCarrick-Walmsley, Ruth and Åkerblad, Peter and Sigvardsson, Mikael and Kadesch, Tom},
  issn         = {0270-7306},
  language     = {eng},
  number       = {11},
  pages        = {3837--3846},
  publisher    = {American Society for Microbiology},
  series       = {Molecular and Cellular Biology},
  title        = {Inhibition of p300/CBP by early B-cell factor},
  url          = {http://dx.doi.org/},
  volume       = {23},
  year         = {2003},
}