Efficient internalization into low-passage glioma cell lines using adenoviruses other than type 5: an approach for improvement of gene delivery to brain tumours
(2004) In Journal of General Virology 84(9). p.2627-2638- Abstract
- There is a need for improvement of the commonly used adenovirus vectors based on serotype 5. This study was performed on three adenovirus serotypes with a CAR-binding motif (Ad4p, Ad5p and Ad17p) and three non-CAR-binding serotypes (Ad11p, Ad16p and Ad21p). The capacity of these alternative adenovirus vector candidates to deliver DNA into low-passage glioma cell lines from seven different donors was evaluated. The non-CAR-binding serotype Ad16p was the most efficient serotype with regard to import of its DNA, as well as initiation of hexon protein expression. Ad16p established hexon expression in 60–80 % of the cell population in gliomas from all donors tested. The other non-CAR-binding serotypes, Ad11p and Ad21p, showed hexon expression... (More)
- There is a need for improvement of the commonly used adenovirus vectors based on serotype 5. This study was performed on three adenovirus serotypes with a CAR-binding motif (Ad4p, Ad5p and Ad17p) and three non-CAR-binding serotypes (Ad11p, Ad16p and Ad21p). The capacity of these alternative adenovirus vector candidates to deliver DNA into low-passage glioma cell lines from seven different donors was evaluated. The non-CAR-binding serotype Ad16p was the most efficient serotype with regard to import of its DNA, as well as initiation of hexon protein expression. Ad16p established hexon expression in 60–80 % of the cell population in gliomas from all donors tested. The other non-CAR-binding serotypes, Ad11p and Ad21p, showed hexon expression in 25–60 and 40–80 % of cells, respectively. The corresponding figure for the best CAR-binding serotype, Ad5p, was only 25–65 %, indicating greater variability between cells from different donors than serotype Ad16p had. The other CAR-binding serotypes, Ad4p and Ad17p, were refractory to some of the gliomas, giving a maximum of only 45 and 40 % hexon expression, respectively, in the most permissive cells. Interestingly, the transduction capacity of the CAR-binding serotypes was not correlated to the level of CAR expression on the cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/141965
- author
- Skog, Johan ; Edlund, Karin ; Widegren, Bengt LU ; Salford, Leif G. ; Wadell, Göran and Mei, Ya-Fang
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of General Virology
- volume
- 84
- issue
- 9
- pages
- 2627 - 2638
- publisher
- Microbiology Society
- external identifiers
-
- pmid:15302956
- wos:000223575500014
- scopus:4544353460
- ISSN
- 1465-2099
- DOI
- 10.1099/vir.0.80084-0
- language
- English
- LU publication?
- yes
- id
- a0245a5c-5625-4e73-9838-39d722540e86 (old id 141965)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15302956&query_hl=136
- date added to LUP
- 2016-04-01 16:06:14
- date last changed
- 2022-01-28 17:17:20
@article{a0245a5c-5625-4e73-9838-39d722540e86, abstract = {{There is a need for improvement of the commonly used adenovirus vectors based on serotype 5. This study was performed on three adenovirus serotypes with a CAR-binding motif (Ad4p, Ad5p and Ad17p) and three non-CAR-binding serotypes (Ad11p, Ad16p and Ad21p). The capacity of these alternative adenovirus vector candidates to deliver DNA into low-passage glioma cell lines from seven different donors was evaluated. The non-CAR-binding serotype Ad16p was the most efficient serotype with regard to import of its DNA, as well as initiation of hexon protein expression. Ad16p established hexon expression in 60–80 % of the cell population in gliomas from all donors tested. The other non-CAR-binding serotypes, Ad11p and Ad21p, showed hexon expression in 25–60 and 40–80 % of cells, respectively. The corresponding figure for the best CAR-binding serotype, Ad5p, was only 25–65 %, indicating greater variability between cells from different donors than serotype Ad16p had. The other CAR-binding serotypes, Ad4p and Ad17p, were refractory to some of the gliomas, giving a maximum of only 45 and 40 % hexon expression, respectively, in the most permissive cells. Interestingly, the transduction capacity of the CAR-binding serotypes was not correlated to the level of CAR expression on the cells.}}, author = {{Skog, Johan and Edlund, Karin and Widegren, Bengt and Salford, Leif G. and Wadell, Göran and Mei, Ya-Fang}}, issn = {{1465-2099}}, language = {{eng}}, number = {{9}}, pages = {{2627--2638}}, publisher = {{Microbiology Society}}, series = {{Journal of General Virology}}, title = {{Efficient internalization into low-passage glioma cell lines using adenoviruses other than type 5: an approach for improvement of gene delivery to brain tumours}}, url = {{https://lup.lub.lu.se/search/files/4568671/624802.pdf}}, doi = {{10.1099/vir.0.80084-0}}, volume = {{84}}, year = {{2004}}, }